Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII
The analysis of tight protein-ligand binding reactions by isothermal titration calorimetry (ITC) and thermal shift assay (TSA) is presented. The binding of radicicol to the N-terminal domain of human heat shock protein 90 (Hsp90aN) and the binding of ethoxzolamide to human carbonic anhydrase (hCAII)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2009-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/10/6/2662/ |
| _version_ | 1811319833372917760 |
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| author | Vilma Michailovienė Jolanta Torresan Jelena Jachno Lina Baranauskienė Jurgita Matulienė Daumantas Matulis Piotras Cimmperman Asta Zubrienė |
| author_facet | Vilma Michailovienė Jolanta Torresan Jelena Jachno Lina Baranauskienė Jurgita Matulienė Daumantas Matulis Piotras Cimmperman Asta Zubrienė |
| author_sort | Vilma Michailovienė |
| collection | DOAJ |
| description | The analysis of tight protein-ligand binding reactions by isothermal titration calorimetry (ITC) and thermal shift assay (TSA) is presented. The binding of radicicol to the N-terminal domain of human heat shock protein 90 (Hsp90aN) and the binding of ethoxzolamide to human carbonic anhydrase (hCAII) were too strong to be measured accurately by direct ITC titration and therefore were measured by displacement ITC and by observing the temperature-denaturation transitions of ligand-free and ligand-bound protein. Stabilization of both proteins by their ligands was profound, increasing the melting temperature by more than 10 ºC, depending on ligand concentration. Analysis of the melting temperature dependence on the protein and ligand concentrations yielded dissociation constants equal to 1 nM and 2 nM for Hsp90aN-radicicol and hCAII-ethoxzolamide, respectively. The ligand-free and ligand-bound protein fractions melt separately, and two melting transitions are observed. This phenomenon is especially pronounced when the ligand concentration is equal to about half the protein concentration. The analysis compares ITC and TSA data, accounts for two transitions and yields the ligand binding constant and the parameters of protein stability, including the Gibbs free energy and the enthalpy of unfolding. |
| first_indexed | 2024-04-13T12:49:25Z |
| format | Article |
| id | doaj.art-83d783e9738044eeafd24862e1d6ea4c |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T12:49:25Z |
| publishDate | 2009-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-83d783e9738044eeafd24862e1d6ea4c2022-12-22T02:46:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672009-06-011062662268010.3390/ijms10062662Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAIIVilma MichailovienėJolanta TorresanJelena JachnoLina BaranauskienėJurgita MatulienėDaumantas MatulisPiotras CimmpermanAsta ZubrienėThe analysis of tight protein-ligand binding reactions by isothermal titration calorimetry (ITC) and thermal shift assay (TSA) is presented. The binding of radicicol to the N-terminal domain of human heat shock protein 90 (Hsp90aN) and the binding of ethoxzolamide to human carbonic anhydrase (hCAII) were too strong to be measured accurately by direct ITC titration and therefore were measured by displacement ITC and by observing the temperature-denaturation transitions of ligand-free and ligand-bound protein. Stabilization of both proteins by their ligands was profound, increasing the melting temperature by more than 10 ºC, depending on ligand concentration. Analysis of the melting temperature dependence on the protein and ligand concentrations yielded dissociation constants equal to 1 nM and 2 nM for Hsp90aN-radicicol and hCAII-ethoxzolamide, respectively. The ligand-free and ligand-bound protein fractions melt separately, and two melting transitions are observed. This phenomenon is especially pronounced when the ligand concentration is equal to about half the protein concentration. The analysis compares ITC and TSA data, accounts for two transitions and yields the ligand binding constant and the parameters of protein stability, including the Gibbs free energy and the enthalpy of unfolding.http://www.mdpi.com/1422-0067/10/6/2662/Hsp90radicicolthermal shift assayisothermal titration calorimetryprotein-ligand bindingcarbonic anhydraseethoxzolamide |
| spellingShingle | Vilma Michailovienė Jolanta Torresan Jelena Jachno Lina Baranauskienė Jurgita Matulienė Daumantas Matulis Piotras Cimmperman Asta Zubrienė Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII International Journal of Molecular Sciences Hsp90 radicicol thermal shift assay isothermal titration calorimetry protein-ligand binding carbonic anhydrase ethoxzolamide |
| title | Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII |
| title_full | Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII |
| title_fullStr | Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII |
| title_full_unstemmed | Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII |
| title_short | Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII |
| title_sort | measurement of nanomolar dissociation constants by titration calorimetry and thermal shift assay radicicol binding to hsp90 and ethoxzolamide binding to caii |
| topic | Hsp90 radicicol thermal shift assay isothermal titration calorimetry protein-ligand binding carbonic anhydrase ethoxzolamide |
| url | http://www.mdpi.com/1422-0067/10/6/2662/ |
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