Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII

Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII

The analysis of tight protein-ligand binding reactions by isothermal titration calorimetry (ITC) and thermal shift assay (TSA) is presented. The binding of radicicol to the N-terminal domain of human heat shock protein 90 (Hsp90aN) and the binding of ethoxzolamide to human carbonic anhydrase (hCAII)...

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Bibliographic Details
Main Authors: Vilma Michailovienė, Jolanta Torresan, Jelena Jachno, Lina Baranauskienė, Jurgita Matulienė, Daumantas Matulis, Piotras Cimmperman, Asta Zubrienė
Format: Article
Language:English
Published: MDPI AG 2009-06-01
Series:International Journal of Molecular Sciences
Subjects:
Hsp90
radicicol
thermal shift assay
isothermal titration calorimetry
protein-ligand binding
carbonic anhydrase
ethoxzolamide
Online Access:http://www.mdpi.com/1422-0067/10/6/2662/

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