Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits
The objective of the present study was to evaluate the influence of the genotypes of two single nucleotide polymorphisms (SNPs) – c.78C>T located in the growth hormone gene (GH) and c.106C>G in the growth hormone receptor gene (GHR) on individual body weight (IBW) during the growing period at...
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| Format: | Article |
| Language: | English |
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Universitat Politècnica de València
2022-03-01
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| Series: | World Rabbit Science |
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| Online Access: | http://polipapers.upv.es/index.php/wrs/article/view/13127 |
| _version_ | 1818393573268127744 |
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| author | Deyana Gencheva Gencheva Krasimir Petrov Velikov Petya Marinova Veleva |
| author_facet | Deyana Gencheva Gencheva Krasimir Petrov Velikov Petya Marinova Veleva |
| author_sort | Deyana Gencheva Gencheva |
| collection | DOAJ |
| description | The objective of the present study was to evaluate the influence of the genotypes of two single nucleotide polymorphisms (SNPs) – c.78C>T located in the growth hormone gene (GH) and c.106C>G in the growth hormone receptor gene (GHR) on individual body weight (IBW) during the growing period at 35, 70 and 90 d of age on a total of 107 weaned Californian breed rabbits. The restriction fragments obtained revealed that 74.8% of the rabbits carrying c.78C>T SNP and 52.3% of the rabbits carrying c.106C>G SNP were heterozygous, which indicated a moderate level of genetic diversity in this Californian population. Association analysis based on a single-gene approach revealed that c.78C>T polymorphism in the GH gene had a significant effect (P<0.05) on the weight at 70 and 90 d of age. The highest IBW (2530.4±66.6 g) was observed in rabbits carrying the c.78C>T TT genotype, and detected individuals were significantly affected by the dominance effect. Significant differences were observed between individuals with homozygous c.106C>G CC genotype and those with heterozygous CG genotype. The highest IBW (2462.0±198.3 g) was observed in rabbits carrying the c.106C>G CC genotype and detected individuals were significantly affected by the additive effect. A total of nine combined genotypes of c.78C>T and c.106C>G SNPs was found in the study, of which only four major groups (CT/CC, CC/CG, CT/CG, and CT/GG) were concerned in the diplotype analysis. Significant differences were observed between individuals with CT/CC and CC/CG genotype combinations, and between those with the CC/CG and CT/GG diplotypes. However, the highest IBW at 90 d of age (2447.2±213.8 g) was observed in rabbits carrying the CT/CC genotype combinations. The highest coefficient of determination found for individual body weight at 90 d of age (R2=10.8%) indicated a high effect of genotype combinations. In conclusion, the results obtained suggested that c.78C>T of GH gene and c.106C>G of GHR gene could be useful candidate genes to improve growth performance in Californian rabbits with potential application in rabbit breeding programmes. |
| first_indexed | 2024-12-14T05:47:27Z |
| format | Article |
| id | doaj.art-83daffda442c451c8468b10bea414e0b |
| institution | Directory Open Access Journal |
| issn | 1257-5011 1989-8886 |
| language | English |
| last_indexed | 2024-12-14T05:47:27Z |
| publishDate | 2022-03-01 |
| publisher | Universitat Politècnica de València |
| record_format | Article |
| series | World Rabbit Science |
| spelling | doaj.art-83daffda442c451c8468b10bea414e0b2022-12-21T23:14:50ZengUniversitat Politècnica de ValènciaWorld Rabbit Science1257-50111989-88862022-03-013019510210.4995/wrs.2022.1312711746Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbitsDeyana Gencheva Gencheva0https://orcid.org/0000-0003-2227-2245Krasimir Petrov Velikov1Petya Marinova Veleva2https://orcid.org/0000-0001-7813-6387Trakia UniversityAgricultural Academy Stara ZagoraTrakia UniversityThe objective of the present study was to evaluate the influence of the genotypes of two single nucleotide polymorphisms (SNPs) – c.78C>T located in the growth hormone gene (GH) and c.106C>G in the growth hormone receptor gene (GHR) on individual body weight (IBW) during the growing period at 35, 70 and 90 d of age on a total of 107 weaned Californian breed rabbits. The restriction fragments obtained revealed that 74.8% of the rabbits carrying c.78C>T SNP and 52.3% of the rabbits carrying c.106C>G SNP were heterozygous, which indicated a moderate level of genetic diversity in this Californian population. Association analysis based on a single-gene approach revealed that c.78C>T polymorphism in the GH gene had a significant effect (P<0.05) on the weight at 70 and 90 d of age. The highest IBW (2530.4±66.6 g) was observed in rabbits carrying the c.78C>T TT genotype, and detected individuals were significantly affected by the dominance effect. Significant differences were observed between individuals with homozygous c.106C>G CC genotype and those with heterozygous CG genotype. The highest IBW (2462.0±198.3 g) was observed in rabbits carrying the c.106C>G CC genotype and detected individuals were significantly affected by the additive effect. A total of nine combined genotypes of c.78C>T and c.106C>G SNPs was found in the study, of which only four major groups (CT/CC, CC/CG, CT/CG, and CT/GG) were concerned in the diplotype analysis. Significant differences were observed between individuals with CT/CC and CC/CG genotype combinations, and between those with the CC/CG and CT/GG diplotypes. However, the highest IBW at 90 d of age (2447.2±213.8 g) was observed in rabbits carrying the CT/CC genotype combinations. The highest coefficient of determination found for individual body weight at 90 d of age (R2=10.8%) indicated a high effect of genotype combinations. In conclusion, the results obtained suggested that c.78C>T of GH gene and c.106C>G of GHR gene could be useful candidate genes to improve growth performance in Californian rabbits with potential application in rabbit breeding programmes.http://polipapers.upv.es/index.php/wrs/article/view/13127californian rabbitsgrowth hormone genegrowth hormone receptor genesnpspcr-rflpbody weight |
| spellingShingle | Deyana Gencheva Gencheva Krasimir Petrov Velikov Petya Marinova Veleva Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits World Rabbit Science californian rabbits growth hormone gene growth hormone receptor gene snps pcr-rflp body weight |
| title | Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits |
| title_full | Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits |
| title_fullStr | Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits |
| title_full_unstemmed | Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits |
| title_short | Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits |
| title_sort | association analysis of nucleotide polymorphisms in growth hormone gh and its receptor ghr with body weight in californian rabbits |
| topic | californian rabbits growth hormone gene growth hormone receptor gene snps pcr-rflp body weight |
| url | http://polipapers.upv.es/index.php/wrs/article/view/13127 |
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