Chayote (<i>Sechium edule</i> (Jacq.) Swartz) Seed as an Unexploited Protein Source: Bio-Functional and Nutritional Quality of Protein Isolates
Chayote seeds have good protein quality and recognized bioactive properties, being still unexplored as a nutraceutical. In this work, chayote seed protein isolates (CSPIs) were prepared by alkaline extraction (AE) and ultrasonic-assisted extraction (UAE) using a probe (20 kHz) or a water bath (40 kH...
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MDPI AG
2023-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/15/2949 |
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| author | Elsa F. Vieira Ana Q. Fontoura Cristina Delerue-Matos |
| author_facet | Elsa F. Vieira Ana Q. Fontoura Cristina Delerue-Matos |
| author_sort | Elsa F. Vieira |
| collection | DOAJ |
| description | Chayote seeds have good protein quality and recognized bioactive properties, being still unexplored as a nutraceutical. In this work, chayote seed protein isolates (CSPIs) were prepared by alkaline extraction (AE) and ultrasonic-assisted extraction (UAE) using a probe (20 kHz) or a water bath (40 kHz), and their physicochemical, functional properties and nutraceutical potential were investigated. For all treatments, protein solutions (10% <i>w</i>/<i>v</i>) were treated for 20 min. The UAE significantly (<i>p</i> < 0.05) improved the protein extraction yield and functional properties (protein solubility, turbidity, and emulsifying and foaming properties) of CSPIs. This effect was more pronounced using a probe sonication device. The CSPI obtained by UAE-20 kHz contained 8.2 ± 0.9% dw of proteins with a balanced amino acid profile, higher content of essential amino acids (315.63 mg/g of protein) and higher protein digestibility (80.3 ± 4.5%). Furthermore, CSPI.UAE-20 kHz exhibited the highest phenolic content (7.22 mg GAE/g dw), antioxidant capacity and α-amylase inhibition (74%, at 100 μg/mL concentration). Overall, these results suggest that ultrasound technology contributed greatly to the corresponding functional and nutritional properties of chayote seed proteins. It would be, therefore, useful to apply this Cucurbitaceae species in food systems, promoting its nutritional and commercial value. |
| first_indexed | 2024-03-11T00:27:27Z |
| format | Article |
| id | doaj.art-83dbe7b127c64fb9848fd729ca4dab39 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T00:27:27Z |
| publishDate | 2023-08-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-83dbe7b127c64fb9848fd729ca4dab392023-11-18T22:55:25ZengMDPI AGFoods2304-81582023-08-011215294910.3390/foods12152949Chayote (<i>Sechium edule</i> (Jacq.) Swartz) Seed as an Unexploited Protein Source: Bio-Functional and Nutritional Quality of Protein IsolatesElsa F. Vieira0Ana Q. Fontoura1Cristina Delerue-Matos2REQUIMTE/LAQV, ISEP, Polytechnic of Porto—School of Engineering, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, PortugalREQUIMTE/LAQV, ISEP, Polytechnic of Porto—School of Engineering, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, PortugalREQUIMTE/LAQV, ISEP, Polytechnic of Porto—School of Engineering, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, PortugalChayote seeds have good protein quality and recognized bioactive properties, being still unexplored as a nutraceutical. In this work, chayote seed protein isolates (CSPIs) were prepared by alkaline extraction (AE) and ultrasonic-assisted extraction (UAE) using a probe (20 kHz) or a water bath (40 kHz), and their physicochemical, functional properties and nutraceutical potential were investigated. For all treatments, protein solutions (10% <i>w</i>/<i>v</i>) were treated for 20 min. The UAE significantly (<i>p</i> < 0.05) improved the protein extraction yield and functional properties (protein solubility, turbidity, and emulsifying and foaming properties) of CSPIs. This effect was more pronounced using a probe sonication device. The CSPI obtained by UAE-20 kHz contained 8.2 ± 0.9% dw of proteins with a balanced amino acid profile, higher content of essential amino acids (315.63 mg/g of protein) and higher protein digestibility (80.3 ± 4.5%). Furthermore, CSPI.UAE-20 kHz exhibited the highest phenolic content (7.22 mg GAE/g dw), antioxidant capacity and α-amylase inhibition (74%, at 100 μg/mL concentration). Overall, these results suggest that ultrasound technology contributed greatly to the corresponding functional and nutritional properties of chayote seed proteins. It would be, therefore, useful to apply this Cucurbitaceae species in food systems, promoting its nutritional and commercial value.https://www.mdpi.com/2304-8158/12/15/2949chayote seedprotein isolateultrasound-assisted extractionfunctional propertiesnutraceuticalin vitro digestibility |
| spellingShingle | Elsa F. Vieira Ana Q. Fontoura Cristina Delerue-Matos Chayote (<i>Sechium edule</i> (Jacq.) Swartz) Seed as an Unexploited Protein Source: Bio-Functional and Nutritional Quality of Protein Isolates Foods chayote seed protein isolate ultrasound-assisted extraction functional properties nutraceutical in vitro digestibility |
| title | Chayote (<i>Sechium edule</i> (Jacq.) Swartz) Seed as an Unexploited Protein Source: Bio-Functional and Nutritional Quality of Protein Isolates |
| title_full | Chayote (<i>Sechium edule</i> (Jacq.) Swartz) Seed as an Unexploited Protein Source: Bio-Functional and Nutritional Quality of Protein Isolates |
| title_fullStr | Chayote (<i>Sechium edule</i> (Jacq.) Swartz) Seed as an Unexploited Protein Source: Bio-Functional and Nutritional Quality of Protein Isolates |
| title_full_unstemmed | Chayote (<i>Sechium edule</i> (Jacq.) Swartz) Seed as an Unexploited Protein Source: Bio-Functional and Nutritional Quality of Protein Isolates |
| title_short | Chayote (<i>Sechium edule</i> (Jacq.) Swartz) Seed as an Unexploited Protein Source: Bio-Functional and Nutritional Quality of Protein Isolates |
| title_sort | chayote i sechium edule i jacq swartz seed as an unexploited protein source bio functional and nutritional quality of protein isolates |
| topic | chayote seed protein isolate ultrasound-assisted extraction functional properties nutraceutical in vitro digestibility |
| url | https://www.mdpi.com/2304-8158/12/15/2949 |
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