The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia
Fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) is a key predictive factor for the prognosis of acute myeloid leukemia (AML). We compared the detection sensitivity of fragment analysis with that of PCR-electrophoresis using MV4-11 (FLT3-ITD) and NKM-1 (FLT3-wild type) cell lines. D...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | Leukemia Research Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213048920300042 |
| _version_ | 1818028600503304192 |
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| author | Masahiro Sakaguchi Nana Nakajima Hiroki Yamaguchi Yuho Najima Katsuhiro Shono Atsushi Marumo Ikuko Omori Yusuke Fujiwara Kazuki Terada Shunsuke Yui Satoshi Wakita Miho Mitaya Kunihito Arai Tomoaki Kitano Noriko Doki Kazuteru Ohashi Koiti Inokuchi |
| author_facet | Masahiro Sakaguchi Nana Nakajima Hiroki Yamaguchi Yuho Najima Katsuhiro Shono Atsushi Marumo Ikuko Omori Yusuke Fujiwara Kazuki Terada Shunsuke Yui Satoshi Wakita Miho Mitaya Kunihito Arai Tomoaki Kitano Noriko Doki Kazuteru Ohashi Koiti Inokuchi |
| author_sort | Masahiro Sakaguchi |
| collection | DOAJ |
| description | Fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) is a key predictive factor for the prognosis of acute myeloid leukemia (AML). We compared the detection sensitivity of fragment analysis with that of PCR-electrophoresis using MV4-11 (FLT3-ITD) and NKM-1 (FLT3-wild type) cell lines. DNA of these cells was mixed at different ratios and subjected to PCR-electrophoresis or fragment analysis. PCR-electrophoresis was found to have an FLT3-ITD allelic ratio (AR) detection limit of 0.034–0.072. Visual inspection of the PCR-electrophoresis revealed a lower detection sensitivity than that of fragment analysis. Therefore, it is essential to conduct fragment analysis when screening for FLT3-ITD. |
| first_indexed | 2024-12-10T05:06:22Z |
| format | Article |
| id | doaj.art-83de8e6f78e142848c9d98dcfaf08594 |
| institution | Directory Open Access Journal |
| issn | 2213-0489 |
| language | English |
| last_indexed | 2024-12-10T05:06:22Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Leukemia Research Reports |
| spelling | doaj.art-83de8e6f78e142848c9d98dcfaf085942022-12-22T02:01:15ZengElsevierLeukemia Research Reports2213-04892020-01-0113100198The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemiaMasahiro Sakaguchi0Nana Nakajima1Hiroki Yamaguchi2Yuho Najima3Katsuhiro Shono4Atsushi Marumo5Ikuko Omori6Yusuke Fujiwara7Kazuki Terada8Shunsuke Yui9Satoshi Wakita10Miho Mitaya11Kunihito Arai12Tomoaki Kitano13Noriko Doki14Kazuteru Ohashi15Koiti Inokuchi16Department of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, Japan; Corresponding author at: Department of Hematology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-Ku, Tokyo, 113-8603, Japan.Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, JapanDepartment of Hematology, Chiba Aoba Municipal Hospital, Chiba, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanHematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, JapanHematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, JapanDepartment of Hematology, Nippon Medical School, Tokyo, JapanFms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) is a key predictive factor for the prognosis of acute myeloid leukemia (AML). We compared the detection sensitivity of fragment analysis with that of PCR-electrophoresis using MV4-11 (FLT3-ITD) and NKM-1 (FLT3-wild type) cell lines. DNA of these cells was mixed at different ratios and subjected to PCR-electrophoresis or fragment analysis. PCR-electrophoresis was found to have an FLT3-ITD allelic ratio (AR) detection limit of 0.034–0.072. Visual inspection of the PCR-electrophoresis revealed a lower detection sensitivity than that of fragment analysis. Therefore, it is essential to conduct fragment analysis when screening for FLT3-ITD.http://www.sciencedirect.com/science/article/pii/S2213048920300042FLT3-ITDAllelic ratioDetection methodSensitivityAcute myeloid leukemia |
| spellingShingle | Masahiro Sakaguchi Nana Nakajima Hiroki Yamaguchi Yuho Najima Katsuhiro Shono Atsushi Marumo Ikuko Omori Yusuke Fujiwara Kazuki Terada Shunsuke Yui Satoshi Wakita Miho Mitaya Kunihito Arai Tomoaki Kitano Noriko Doki Kazuteru Ohashi Koiti Inokuchi The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia Leukemia Research Reports FLT3-ITD Allelic ratio Detection method Sensitivity Acute myeloid leukemia |
| title | The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia |
| title_full | The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia |
| title_fullStr | The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia |
| title_full_unstemmed | The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia |
| title_short | The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia |
| title_sort | sensitivity of the flt3 itd detection method is an important consideration when diagnosing acute myeloid leukemia |
| topic | FLT3-ITD Allelic ratio Detection method Sensitivity Acute myeloid leukemia |
| url | http://www.sciencedirect.com/science/article/pii/S2213048920300042 |
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