Evaluation of Methanotroph (<i>Methylococcus capsulatus</i>, Bath) Bacteria Protein as an Alternative to Fish Meal in the Diet of Juvenile American Eel (<i>Anguilla rostrata</i>)
This study was conducted to evaluate the effects of replacing fish meal (FM) with methanotroph (<i>Methylococcus capsulatus</i>, Bath) bacteria protein (MBP) in the diets of the juvenile American eel (<i>Anguilla rostrata</i>). Trial fish were randomly divided into the MBP0 g...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/4/681 |
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| author | Wenqi Lu Haixia Yu Ying Liang Shaowei Zhai |
| author_facet | Wenqi Lu Haixia Yu Ying Liang Shaowei Zhai |
| author_sort | Wenqi Lu |
| collection | DOAJ |
| description | This study was conducted to evaluate the effects of replacing fish meal (FM) with methanotroph (<i>Methylococcus capsulatus</i>, Bath) bacteria protein (MBP) in the diets of the juvenile American eel (<i>Anguilla rostrata</i>). Trial fish were randomly divided into the MBP0 group, MBP6 group, MBP12 group, and MBP18 group fed the diets with MBP replacing FM at levels of 0, 6%, 12%, and 18%, respectively. The trial lasted for ten weeks. There were no significant differences in weight gain or feed utilization among the MBP0, MBP6, and MBP12 groups (except for the feeding rate in the MBP12 group). Compared with the MBP0 group, the D-lactate level and diamine oxidase activity in the serum were significantly elevated in the MBP12 and MBP18 groups. In terms of non-specific immunity parameters in serum, the alkaline phosphatase activity was significantly decreased in the MBP18 group, and the complement 3 level was significantly elevated in the MBP12 and MBP18 groups. The activities of lipase and protease in the intestine were significantly decreased in the MBP12 and MBP18 groups. Compared with the MBP0 group, the total antioxidant capacity and activities of superoxide dismutase, catalase, and glutathione peroxidase in the intestine were significantly decreased in the MBP18 group, while the malondialdehyde level was significantly increased. The villus height, muscular thickness, and microvillus density were significantly decreased in the MBP12 and MBP18 groups. There were no significant differences in the foresaid parameters between the MBP0 group and the MBP6 group. The intestinal microbiota of the MBP6 group was beneficially regulated to maintain similar growth and health status with the MBP0 group. The adverse effects on the intestinal microbiota were reflected in the MBP18 group. In conclusion, MBP could successfully replace 6% of FM in the diet without adversely affecting the growth performance, serum biochemical parameters, and intestinal health of juvenile American eels. |
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| last_indexed | 2024-03-11T09:16:08Z |
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| series | Animals |
| spelling | doaj.art-83df764fbca346f6a0df3ba7b5429c252023-11-16T18:40:03ZengMDPI AGAnimals2076-26152023-02-0113468110.3390/ani13040681Evaluation of Methanotroph (<i>Methylococcus capsulatus</i>, Bath) Bacteria Protein as an Alternative to Fish Meal in the Diet of Juvenile American Eel (<i>Anguilla rostrata</i>)Wenqi Lu0Haixia Yu1Ying Liang2Shaowei Zhai3Engineering Research Center of the Modern Industry Technology for Eel, Ministry of Education, Fisheries College of Jimei University, Xiamen 361021, ChinaEngineering Research Center of the Modern Industry Technology for Eel, Ministry of Education, Fisheries College of Jimei University, Xiamen 361021, ChinaEngineering Research Center of the Modern Industry Technology for Eel, Ministry of Education, Fisheries College of Jimei University, Xiamen 361021, ChinaEngineering Research Center of the Modern Industry Technology for Eel, Ministry of Education, Fisheries College of Jimei University, Xiamen 361021, ChinaThis study was conducted to evaluate the effects of replacing fish meal (FM) with methanotroph (<i>Methylococcus capsulatus</i>, Bath) bacteria protein (MBP) in the diets of the juvenile American eel (<i>Anguilla rostrata</i>). Trial fish were randomly divided into the MBP0 group, MBP6 group, MBP12 group, and MBP18 group fed the diets with MBP replacing FM at levels of 0, 6%, 12%, and 18%, respectively. The trial lasted for ten weeks. There were no significant differences in weight gain or feed utilization among the MBP0, MBP6, and MBP12 groups (except for the feeding rate in the MBP12 group). Compared with the MBP0 group, the D-lactate level and diamine oxidase activity in the serum were significantly elevated in the MBP12 and MBP18 groups. In terms of non-specific immunity parameters in serum, the alkaline phosphatase activity was significantly decreased in the MBP18 group, and the complement 3 level was significantly elevated in the MBP12 and MBP18 groups. The activities of lipase and protease in the intestine were significantly decreased in the MBP12 and MBP18 groups. Compared with the MBP0 group, the total antioxidant capacity and activities of superoxide dismutase, catalase, and glutathione peroxidase in the intestine were significantly decreased in the MBP18 group, while the malondialdehyde level was significantly increased. The villus height, muscular thickness, and microvillus density were significantly decreased in the MBP12 and MBP18 groups. There were no significant differences in the foresaid parameters between the MBP0 group and the MBP6 group. The intestinal microbiota of the MBP6 group was beneficially regulated to maintain similar growth and health status with the MBP0 group. The adverse effects on the intestinal microbiota were reflected in the MBP18 group. In conclusion, MBP could successfully replace 6% of FM in the diet without adversely affecting the growth performance, serum biochemical parameters, and intestinal health of juvenile American eels.https://www.mdpi.com/2076-2615/13/4/681methanotroph bacteria proteingrowthserum immunityintestinal healthintestinal microbiota<i>Anguilla rostrata</i> |
| spellingShingle | Wenqi Lu Haixia Yu Ying Liang Shaowei Zhai Evaluation of Methanotroph (<i>Methylococcus capsulatus</i>, Bath) Bacteria Protein as an Alternative to Fish Meal in the Diet of Juvenile American Eel (<i>Anguilla rostrata</i>) Animals methanotroph bacteria protein growth serum immunity intestinal health intestinal microbiota <i>Anguilla rostrata</i> |
| title | Evaluation of Methanotroph (<i>Methylococcus capsulatus</i>, Bath) Bacteria Protein as an Alternative to Fish Meal in the Diet of Juvenile American Eel (<i>Anguilla rostrata</i>) |
| title_full | Evaluation of Methanotroph (<i>Methylococcus capsulatus</i>, Bath) Bacteria Protein as an Alternative to Fish Meal in the Diet of Juvenile American Eel (<i>Anguilla rostrata</i>) |
| title_fullStr | Evaluation of Methanotroph (<i>Methylococcus capsulatus</i>, Bath) Bacteria Protein as an Alternative to Fish Meal in the Diet of Juvenile American Eel (<i>Anguilla rostrata</i>) |
| title_full_unstemmed | Evaluation of Methanotroph (<i>Methylococcus capsulatus</i>, Bath) Bacteria Protein as an Alternative to Fish Meal in the Diet of Juvenile American Eel (<i>Anguilla rostrata</i>) |
| title_short | Evaluation of Methanotroph (<i>Methylococcus capsulatus</i>, Bath) Bacteria Protein as an Alternative to Fish Meal in the Diet of Juvenile American Eel (<i>Anguilla rostrata</i>) |
| title_sort | evaluation of methanotroph i methylococcus capsulatus i bath bacteria protein as an alternative to fish meal in the diet of juvenile american eel i anguilla rostrata i |
| topic | methanotroph bacteria protein growth serum immunity intestinal health intestinal microbiota <i>Anguilla rostrata</i> |
| url | https://www.mdpi.com/2076-2615/13/4/681 |
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