Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida
Summary In contrast to the current paradigm of using microbial mono‐cultures in most biotechnological applications, increasing efforts are being directed towards engineering mixed‐species consortia to perform functions that are difficult to programme into individual strains. In this work, we develop...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-07-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13544 |
| _version_ | 1818741700635394048 |
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| author | Derek T. Fedeson Pia Saake Patricia Calero Pablo Iván Nikel Daniel C. Ducat |
| author_facet | Derek T. Fedeson Pia Saake Patricia Calero Pablo Iván Nikel Daniel C. Ducat |
| author_sort | Derek T. Fedeson |
| collection | DOAJ |
| description | Summary In contrast to the current paradigm of using microbial mono‐cultures in most biotechnological applications, increasing efforts are being directed towards engineering mixed‐species consortia to perform functions that are difficult to programme into individual strains. In this work, we developed a synthetic microbial consortium composed of two genetically engineered microbes, a cyanobacterium (Synechococcus elongatus PCC 7942) and a heterotrophic bacterium (Pseudomonas putida EM173). These microbial species specialize in the co‐culture: cyanobacteria fix CO2 through photosynthetic metabolism and secrete sufficient carbohydrates to support the growth and active metabolism of P. putida, which has been engineered to consume sucrose and to degrade the environmental pollutant 2,4‐dinitrotoluene (2,4‐DNT). By encapsulating S. elongatus within a barium–alginate hydrogel, cyanobacterial cells were protected from the toxic effects of 2,4‐DNT, enhancing the performance of the co‐culture. The synthetic consortium was able to convert 2,4‐DNT with light and CO2 as key inputs, and its catalytic performance was stable over time. Furthermore, cycling this synthetic consortium through low nitrogen medium promoted the sucrose‐dependent accumulation of polyhydroxyalkanoate, an added‐value biopolymer, in the engineered P. putida strain. Altogether, the synthetic consortium displayed the capacity to remediate the industrial pollutant 2,4‐DNT while simultaneously synthesizing biopolymers using light and CO2 as the primary inputs. |
| first_indexed | 2024-12-18T02:00:48Z |
| format | Article |
| id | doaj.art-83e0c76552964e55a474efb968fb66d0 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-18T02:00:48Z |
| publishDate | 2020-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-83e0c76552964e55a474efb968fb66d02022-12-21T21:24:47ZengWileyMicrobial Biotechnology1751-79152020-07-01134997101110.1111/1751-7915.13544Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putidaDerek T. Fedeson0Pia Saake1Patricia Calero2Pablo Iván Nikel3Daniel C. Ducat4DOE‐MSU Plant Research Laboratories Michigan State University East Lansing MI USAHeinrich‐Heine Universität Düsseldorf GermanyThe Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark Kgs Lyngby DenmarkThe Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark Kgs Lyngby DenmarkDOE‐MSU Plant Research Laboratories Michigan State University East Lansing MI USASummary In contrast to the current paradigm of using microbial mono‐cultures in most biotechnological applications, increasing efforts are being directed towards engineering mixed‐species consortia to perform functions that are difficult to programme into individual strains. In this work, we developed a synthetic microbial consortium composed of two genetically engineered microbes, a cyanobacterium (Synechococcus elongatus PCC 7942) and a heterotrophic bacterium (Pseudomonas putida EM173). These microbial species specialize in the co‐culture: cyanobacteria fix CO2 through photosynthetic metabolism and secrete sufficient carbohydrates to support the growth and active metabolism of P. putida, which has been engineered to consume sucrose and to degrade the environmental pollutant 2,4‐dinitrotoluene (2,4‐DNT). By encapsulating S. elongatus within a barium–alginate hydrogel, cyanobacterial cells were protected from the toxic effects of 2,4‐DNT, enhancing the performance of the co‐culture. The synthetic consortium was able to convert 2,4‐DNT with light and CO2 as key inputs, and its catalytic performance was stable over time. Furthermore, cycling this synthetic consortium through low nitrogen medium promoted the sucrose‐dependent accumulation of polyhydroxyalkanoate, an added‐value biopolymer, in the engineered P. putida strain. Altogether, the synthetic consortium displayed the capacity to remediate the industrial pollutant 2,4‐DNT while simultaneously synthesizing biopolymers using light and CO2 as the primary inputs.https://doi.org/10.1111/1751-7915.13544 |
| spellingShingle | Derek T. Fedeson Pia Saake Patricia Calero Pablo Iván Nikel Daniel C. Ducat Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida Microbial Biotechnology |
| title | Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida |
| title_full | Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida |
| title_fullStr | Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida |
| title_full_unstemmed | Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida |
| title_short | Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida |
| title_sort | biotransformation of 2 4 dinitrotoluene in a phototrophic co culture of engineered synechococcus elongatus and pseudomonas putida |
| url | https://doi.org/10.1111/1751-7915.13544 |
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