Association of Genetic Polymorphisms in <i>TLR3</i>, <i>TLR4</i>, <i>TLR7</i>, and <i>TLR8</i> with the Clinical Forms of Dengue in Patients from Veracruz, Mexico
Dengue manifestations range from a mild form, dengue fever (DF), to more severe forms such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The ability of the host to present one of these clinical forms could be related to polymorphisms located in genes of the Toll-like receptors (...
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MDPI AG
2020-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/11/1230 |
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| author | Araceli Posadas-Mondragón José Leopoldo Aguilar-Faisal Gerardo Zuñiga Jonathan Javier Magaña José Angel Santiago-Cruz Edith Guillén-Salomón Verónica Alcántara-Farfán María Luisa Arellano-Flores Juan Santiago Salas-Benito Rocío M. Neri-Bazán Lucero Luna-Rojas Amanda Marineth Avila-Trejo Adolfo Chávez-Negrete |
| author_facet | Araceli Posadas-Mondragón José Leopoldo Aguilar-Faisal Gerardo Zuñiga Jonathan Javier Magaña José Angel Santiago-Cruz Edith Guillén-Salomón Verónica Alcántara-Farfán María Luisa Arellano-Flores Juan Santiago Salas-Benito Rocío M. Neri-Bazán Lucero Luna-Rojas Amanda Marineth Avila-Trejo Adolfo Chávez-Negrete |
| author_sort | Araceli Posadas-Mondragón |
| collection | DOAJ |
| description | Dengue manifestations range from a mild form, dengue fever (DF), to more severe forms such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The ability of the host to present one of these clinical forms could be related to polymorphisms located in genes of the Toll-like receptors (TLRs) which activate the pro-inflammatory response. Therefore, the genotyping of single nucleotide genetic polymorphisms (SNPs) in <i>TLR3</i> (rs3775291 and rs6552950), <i>TLR4</i> (rs2737190, rs10759932, rs4986790, rs4986791, rs11536865, and rs10983755), <i>TLR7</i> (rs179008 and rs3853839), and <i>TLR8</i> (rs3764880, rs5741883, rs4830805, and rs1548731) was carried out in non-genetically related DHF patients, DF patients, and general population (GP) subjects. The SNPs were analyzed by real-time PCR by genotyping assays from Applied Biosystems<sup>®</sup>. The codominance model showed that dengue patients had a lower probability of presenting the <i>TLR4</i>-rs2737190-G/G genotype (odds ratio (OR) (95% CI) = 0.34 (0.14–0.8), <i>p</i> = 0.038). Dengue patients showed a lower probability of presenting <i>TLR4</i>-rs11536865-G/C genotype (OR (95% CI) = 0.19 (0.05–0.73), <i>p</i> = 0.0092) and had a high probability of presenting the TACG haplotype, but lower probability of presenting the TGCG haplotype in the <i>TLR4</i> compared to GP individuals (OR (95% CI) = 0.55 (0.35–0.86), <i>p</i> = 0.0084). In conclusion, the <i>TLR4</i>-rs2737190-G/G and <i>TLR4</i>-rs11536865-G/C genotypes and TGCG haplotype were associated with protection from dengue. |
| first_indexed | 2024-03-10T15:14:01Z |
| format | Article |
| id | doaj.art-83e2c12bda5c4a8db7d5f761892cf238 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T15:14:01Z |
| publishDate | 2020-10-01 |
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| record_format | Article |
| series | Viruses |
| spelling | doaj.art-83e2c12bda5c4a8db7d5f761892cf2382023-11-20T19:07:16ZengMDPI AGViruses1999-49152020-10-011211123010.3390/v12111230Association of Genetic Polymorphisms in <i>TLR3</i>, <i>TLR4</i>, <i>TLR7</i>, and <i>TLR8</i> with the Clinical Forms of Dengue in Patients from Veracruz, MexicoAraceli Posadas-Mondragón0José Leopoldo Aguilar-Faisal1Gerardo Zuñiga2Jonathan Javier Magaña3José Angel Santiago-Cruz4Edith Guillén-Salomón5Verónica Alcántara-Farfán6María Luisa Arellano-Flores7Juan Santiago Salas-Benito8Rocío M. Neri-Bazán9Lucero Luna-Rojas10Amanda Marineth Avila-Trejo11Adolfo Chávez-Negrete12Laboratorio de Medicina de Conservación de la Sección de Estudios de Posgrado e Investigación, Escuela Superior Medicina, Instituto Politécnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Delegación Miguel Hidalgo, Ciudad de México 11340, MexicoLaboratorio de Medicina de Conservación de la Sección de Estudios de Posgrado e Investigación, Escuela Superior Medicina, Instituto Politécnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Delegación Miguel Hidalgo, Ciudad de México 11340, MexicoDepartamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala, Colonia Casco de Santo Tomas, Miguel Hidalgo, Ciudad de México 11340, MexicoLaboratorio de Medicina Genómica, Departamento de Genética, Instituto Nacional de Rehabilitación-LGII, Calzada México Xochimilco No. 289, Colonia Arenal de Guadalupe, Ciudad de México 14389, MexicoDepartamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala, Colonia Casco de Santo Tomas, Delegación Miguel Hidalgo, Ciudad de Mexico 11340, MexicoCoordinación de Planeación y Enlace Institucional, Delegación Veracruz Norte, Instituto Mexicano del Seguro Social, Lomas del Estadio S/N Xalapa, Veracruz 91090, MexicoDepartamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala, Colonia Casco de Santo Tomas, Delegación Miguel Hidalgo, Ciudad de México 11340, MexicoUnidad de Investigación en Enfermedades Endocrinas, Hospital de Especialidades, Centro Médico, Nacional siglo XXI, Instituto Mexicano del Seguro Social, Cuauhtémoc 330, Colonia Doctores, Delegación Cuauhtémoc, Ciudad de México 06720, MexicoLaboratorio de Biomedicina Molecular III (Virología) de la Sección de Estudios de Posgrado e Investigación, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 239, La Escalera, Gustavo A. Madero, Ciudad de México 07320, MexicoLaboratorio de Medicina de Conservación de la Sección de Estudios de Posgrado e Investigación, Escuela Superior Medicina, Instituto Politécnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Delegación Miguel Hidalgo, Ciudad de México 11340, MexicoLaboratorio de Medicina de Conservación de la Sección de Estudios de Posgrado e Investigación, Escuela Superior Medicina, Instituto Politécnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Delegación Miguel Hidalgo, Ciudad de México 11340, MexicoLaboratorio de Medicina de Conservación de la Sección de Estudios de Posgrado e Investigación, Escuela Superior Medicina, Instituto Politécnico Nacional, Plan de San Luis, Colonia Casco de Santo Tomas, Delegación Miguel Hidalgo, Ciudad de México 11340, MexicoCoordinación de Educación Médica Continua, Comité Normativo Nacional de Medicina General, Cuauhtémoc 330, Colonia Doctores, Delegación Cuauhtémoc CDMX 06720, MexicoDengue manifestations range from a mild form, dengue fever (DF), to more severe forms such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The ability of the host to present one of these clinical forms could be related to polymorphisms located in genes of the Toll-like receptors (TLRs) which activate the pro-inflammatory response. Therefore, the genotyping of single nucleotide genetic polymorphisms (SNPs) in <i>TLR3</i> (rs3775291 and rs6552950), <i>TLR4</i> (rs2737190, rs10759932, rs4986790, rs4986791, rs11536865, and rs10983755), <i>TLR7</i> (rs179008 and rs3853839), and <i>TLR8</i> (rs3764880, rs5741883, rs4830805, and rs1548731) was carried out in non-genetically related DHF patients, DF patients, and general population (GP) subjects. The SNPs were analyzed by real-time PCR by genotyping assays from Applied Biosystems<sup>®</sup>. The codominance model showed that dengue patients had a lower probability of presenting the <i>TLR4</i>-rs2737190-G/G genotype (odds ratio (OR) (95% CI) = 0.34 (0.14–0.8), <i>p</i> = 0.038). Dengue patients showed a lower probability of presenting <i>TLR4</i>-rs11536865-G/C genotype (OR (95% CI) = 0.19 (0.05–0.73), <i>p</i> = 0.0092) and had a high probability of presenting the TACG haplotype, but lower probability of presenting the TGCG haplotype in the <i>TLR4</i> compared to GP individuals (OR (95% CI) = 0.55 (0.35–0.86), <i>p</i> = 0.0084). In conclusion, the <i>TLR4</i>-rs2737190-G/G and <i>TLR4</i>-rs11536865-G/C genotypes and TGCG haplotype were associated with protection from dengue.https://www.mdpi.com/1999-4915/12/11/1230toll-like receptordenguegene polymorphismsdengue feverdengue hemorrhagic feverhuman genetic susceptibility |
| spellingShingle | Araceli Posadas-Mondragón José Leopoldo Aguilar-Faisal Gerardo Zuñiga Jonathan Javier Magaña José Angel Santiago-Cruz Edith Guillén-Salomón Verónica Alcántara-Farfán María Luisa Arellano-Flores Juan Santiago Salas-Benito Rocío M. Neri-Bazán Lucero Luna-Rojas Amanda Marineth Avila-Trejo Adolfo Chávez-Negrete Association of Genetic Polymorphisms in <i>TLR3</i>, <i>TLR4</i>, <i>TLR7</i>, and <i>TLR8</i> with the Clinical Forms of Dengue in Patients from Veracruz, Mexico Viruses toll-like receptor dengue gene polymorphisms dengue fever dengue hemorrhagic fever human genetic susceptibility |
| title | Association of Genetic Polymorphisms in <i>TLR3</i>, <i>TLR4</i>, <i>TLR7</i>, and <i>TLR8</i> with the Clinical Forms of Dengue in Patients from Veracruz, Mexico |
| title_full | Association of Genetic Polymorphisms in <i>TLR3</i>, <i>TLR4</i>, <i>TLR7</i>, and <i>TLR8</i> with the Clinical Forms of Dengue in Patients from Veracruz, Mexico |
| title_fullStr | Association of Genetic Polymorphisms in <i>TLR3</i>, <i>TLR4</i>, <i>TLR7</i>, and <i>TLR8</i> with the Clinical Forms of Dengue in Patients from Veracruz, Mexico |
| title_full_unstemmed | Association of Genetic Polymorphisms in <i>TLR3</i>, <i>TLR4</i>, <i>TLR7</i>, and <i>TLR8</i> with the Clinical Forms of Dengue in Patients from Veracruz, Mexico |
| title_short | Association of Genetic Polymorphisms in <i>TLR3</i>, <i>TLR4</i>, <i>TLR7</i>, and <i>TLR8</i> with the Clinical Forms of Dengue in Patients from Veracruz, Mexico |
| title_sort | association of genetic polymorphisms in i tlr3 i i tlr4 i i tlr7 i and i tlr8 i with the clinical forms of dengue in patients from veracruz mexico |
| topic | toll-like receptor dengue gene polymorphisms dengue fever dengue hemorrhagic fever human genetic susceptibility |
| url | https://www.mdpi.com/1999-4915/12/11/1230 |
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