Dietary Protected Butyrate Supplementation of Broilers Modulates Intestinal Tight Junction Proteins and Stimulates Endogenous Production of Short Chain Fatty Acids in the Caecum
Short chain fatty acid (SCFA) butyrate has various beneficial effects on the gut microbiota as well as on the overall health status and metabolism of the host organism. The modulatory role of butyrate on gut barrier integrity reflected by tight junction protein expression has been already described...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/15/1940 |
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| author | Gábor Mátis Máté Mackei Bart Boomsma Hedvig Fébel Katarzyna Nadolna Łukasz Szymański Joan E. Edwards Zsuzsanna Neogrády Krzysztof Kozłowski |
| author_facet | Gábor Mátis Máté Mackei Bart Boomsma Hedvig Fébel Katarzyna Nadolna Łukasz Szymański Joan E. Edwards Zsuzsanna Neogrády Krzysztof Kozłowski |
| author_sort | Gábor Mátis |
| collection | DOAJ |
| description | Short chain fatty acid (SCFA) butyrate has various beneficial effects on the gut microbiota as well as on the overall health status and metabolism of the host organism. The modulatory role of butyrate on gut barrier integrity reflected by tight junction protein expression has been already described in mammalian species. However, there is limited information available regarding chickens. Therefore, the main aim of this study was to monitor the effects of protected butyrate on claudin barrier protein and monocarboxylate transporter 1 abundance in different gastrointestinal segments of chickens as well as the growth performance of broiler chickens. The effect of protected butyrate on the caecal microbiota was monitored by quantifying the concentrations of total eubacteria and key enzymes of butyrate production. Furthermore, intestinal SCFA concentrations were also measured. Based on the data obtained, protected butyrate increased the overall performance as well as the barrier integrity of various gut segments. Protected butyrate also positively affected the SCFA concentration and composition. These findings provide valuable insight into the complex effects of protected butyrate on broiler gut health, highlighting the beneficial effects in improving intestinal barrier integrity and performance parameters. |
| first_indexed | 2024-03-09T05:40:17Z |
| format | Article |
| id | doaj.art-83e340c3cab44b45adc3c0af96e25ed9 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T05:40:17Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-83e340c3cab44b45adc3c0af96e25ed92023-12-03T12:25:59ZengMDPI AGAnimals2076-26152022-07-011215194010.3390/ani12151940Dietary Protected Butyrate Supplementation of Broilers Modulates Intestinal Tight Junction Proteins and Stimulates Endogenous Production of Short Chain Fatty Acids in the CaecumGábor Mátis0Máté Mackei1Bart Boomsma2Hedvig Fébel3Katarzyna Nadolna4Łukasz Szymański5Joan E. Edwards6Zsuzsanna Neogrády7Krzysztof Kozłowski8Division of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine, István utca 2, H-1078 Budapest, HungaryDivision of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine, István utca 2, H-1078 Budapest, HungaryPalital Feed Additives B.V., De Tweede Geerden, 5334 LH Velddriel, The NetherlandsNutrition Physiology Research Group, Institute of Physiology and Nutrition, Hungarian University of Agriculture and Life Sciences, Gesztenyés Str. 1, H-2053 Herceghalom, HungaryDepartment of Poultry Science and Apiculture, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, PolandDepartment of Poultry Science and Apiculture, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, PolandPalital Feed Additives B.V., De Tweede Geerden, 5334 LH Velddriel, The NetherlandsDivision of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine, István utca 2, H-1078 Budapest, HungaryDepartment of Poultry Science and Apiculture, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, PolandShort chain fatty acid (SCFA) butyrate has various beneficial effects on the gut microbiota as well as on the overall health status and metabolism of the host organism. The modulatory role of butyrate on gut barrier integrity reflected by tight junction protein expression has been already described in mammalian species. However, there is limited information available regarding chickens. Therefore, the main aim of this study was to monitor the effects of protected butyrate on claudin barrier protein and monocarboxylate transporter 1 abundance in different gastrointestinal segments of chickens as well as the growth performance of broiler chickens. The effect of protected butyrate on the caecal microbiota was monitored by quantifying the concentrations of total eubacteria and key enzymes of butyrate production. Furthermore, intestinal SCFA concentrations were also measured. Based on the data obtained, protected butyrate increased the overall performance as well as the barrier integrity of various gut segments. Protected butyrate also positively affected the SCFA concentration and composition. These findings provide valuable insight into the complex effects of protected butyrate on broiler gut health, highlighting the beneficial effects in improving intestinal barrier integrity and performance parameters.https://www.mdpi.com/2076-2615/12/15/1940butyric acidorganic acidspoultrychickengrowth performancegut |
| spellingShingle | Gábor Mátis Máté Mackei Bart Boomsma Hedvig Fébel Katarzyna Nadolna Łukasz Szymański Joan E. Edwards Zsuzsanna Neogrády Krzysztof Kozłowski Dietary Protected Butyrate Supplementation of Broilers Modulates Intestinal Tight Junction Proteins and Stimulates Endogenous Production of Short Chain Fatty Acids in the Caecum Animals butyric acid organic acids poultry chicken growth performance gut |
| title | Dietary Protected Butyrate Supplementation of Broilers Modulates Intestinal Tight Junction Proteins and Stimulates Endogenous Production of Short Chain Fatty Acids in the Caecum |
| title_full | Dietary Protected Butyrate Supplementation of Broilers Modulates Intestinal Tight Junction Proteins and Stimulates Endogenous Production of Short Chain Fatty Acids in the Caecum |
| title_fullStr | Dietary Protected Butyrate Supplementation of Broilers Modulates Intestinal Tight Junction Proteins and Stimulates Endogenous Production of Short Chain Fatty Acids in the Caecum |
| title_full_unstemmed | Dietary Protected Butyrate Supplementation of Broilers Modulates Intestinal Tight Junction Proteins and Stimulates Endogenous Production of Short Chain Fatty Acids in the Caecum |
| title_short | Dietary Protected Butyrate Supplementation of Broilers Modulates Intestinal Tight Junction Proteins and Stimulates Endogenous Production of Short Chain Fatty Acids in the Caecum |
| title_sort | dietary protected butyrate supplementation of broilers modulates intestinal tight junction proteins and stimulates endogenous production of short chain fatty acids in the caecum |
| topic | butyric acid organic acids poultry chicken growth performance gut |
| url | https://www.mdpi.com/2076-2615/12/15/1940 |
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