Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis
<i>Arachis hypogaea</i> abscisic acid transporter like-1 (AhATL1) modulates abscisic acid (ABA) sensitivity by specifically influencing the importing of ABA into cells, and is a key player in plant stress responses. However, there is limited information on ABA transporters in crops. In t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/7/3398 |
| _version_ | 1797539965638279168 |
|---|---|
| author | Ming Qin Xiaoyan Li Shaohua Tang Yinglin Huang Ling Li Bo Hu |
| author_facet | Ming Qin Xiaoyan Li Shaohua Tang Yinglin Huang Ling Li Bo Hu |
| author_sort | Ming Qin |
| collection | DOAJ |
| description | <i>Arachis hypogaea</i> abscisic acid transporter like-1 (AhATL1) modulates abscisic acid (ABA) sensitivity by specifically influencing the importing of ABA into cells, and is a key player in plant stress responses. However, there is limited information on ABA transporters in crops. In this study, we found that the level of <i>AhATL1</i> expression and AhATL1 distribution increased more rapidly in the second drought (D2) compared with in the first drought (D1). Compared with the first recovery (R1), the <i>AhATL1</i> expression level and ABA content remained at a higher level during the second recovery (R2). The heterologous overexpression of <i>AhATL1</i> in <i>Arabidopsis</i> changed the expression pattern of certain memory genes and changed the post response gene type into the memory gene type. Regarding the proline and water content of Col (<i>Arabidopsis thaliana</i> L. Heynh., Col-0), <i>atabcg22,</i> and AhATL1-OX during drought training, the second drought (D2) was more severe than the first drought (D1), which was more conducive to maintaining the cell osmotic balance and resisting drought. In summary, drought stress memory resulted in a rapid increase in the <i>AhATL1</i> expression and AhATL1 distribution level, and then raised the endogenous ABA content and changed the post response gene type into the memory gene type, which enhanced the drought resistance and recovery ability. |
| first_indexed | 2024-03-10T12:54:18Z |
| format | Article |
| id | doaj.art-83ea070ef17643de90da2fc4ad297581 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:54:18Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-83ea070ef17643de90da2fc4ad2975812023-11-21T12:05:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227339810.3390/ijms22073398Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in ArabidopsisMing Qin0Xiaoyan Li1Shaohua Tang2Yinglin Huang3Ling Li4Bo Hu5Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China<i>Arachis hypogaea</i> abscisic acid transporter like-1 (AhATL1) modulates abscisic acid (ABA) sensitivity by specifically influencing the importing of ABA into cells, and is a key player in plant stress responses. However, there is limited information on ABA transporters in crops. In this study, we found that the level of <i>AhATL1</i> expression and AhATL1 distribution increased more rapidly in the second drought (D2) compared with in the first drought (D1). Compared with the first recovery (R1), the <i>AhATL1</i> expression level and ABA content remained at a higher level during the second recovery (R2). The heterologous overexpression of <i>AhATL1</i> in <i>Arabidopsis</i> changed the expression pattern of certain memory genes and changed the post response gene type into the memory gene type. Regarding the proline and water content of Col (<i>Arabidopsis thaliana</i> L. Heynh., Col-0), <i>atabcg22,</i> and AhATL1-OX during drought training, the second drought (D2) was more severe than the first drought (D1), which was more conducive to maintaining the cell osmotic balance and resisting drought. In summary, drought stress memory resulted in a rapid increase in the <i>AhATL1</i> expression and AhATL1 distribution level, and then raised the endogenous ABA content and changed the post response gene type into the memory gene type, which enhanced the drought resistance and recovery ability.https://www.mdpi.com/1422-0067/22/7/3398AhATL1ABAmemory genetolerancedrought stress memorypeanut |
| spellingShingle | Ming Qin Xiaoyan Li Shaohua Tang Yinglin Huang Ling Li Bo Hu Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis International Journal of Molecular Sciences AhATL1 ABA memory gene tolerance drought stress memory peanut |
| title | Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis |
| title_full | Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis |
| title_fullStr | Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis |
| title_full_unstemmed | Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis |
| title_short | Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis |
| title_sort | expression of ahatl1 an aba transport factor gene from peanut is affected by altered memory gene expression patterns and increased tolerance to drought stress in arabidopsis |
| topic | AhATL1 ABA memory gene tolerance drought stress memory peanut |
| url | https://www.mdpi.com/1422-0067/22/7/3398 |
| work_keys_str_mv | AT mingqin expressionofahatl1anabatransportfactorgenefrompeanutisaffectedbyalteredmemorygeneexpressionpatternsandincreasedtolerancetodroughtstressinarabidopsis AT xiaoyanli expressionofahatl1anabatransportfactorgenefrompeanutisaffectedbyalteredmemorygeneexpressionpatternsandincreasedtolerancetodroughtstressinarabidopsis AT shaohuatang expressionofahatl1anabatransportfactorgenefrompeanutisaffectedbyalteredmemorygeneexpressionpatternsandincreasedtolerancetodroughtstressinarabidopsis AT yinglinhuang expressionofahatl1anabatransportfactorgenefrompeanutisaffectedbyalteredmemorygeneexpressionpatternsandincreasedtolerancetodroughtstressinarabidopsis AT lingli expressionofahatl1anabatransportfactorgenefrompeanutisaffectedbyalteredmemorygeneexpressionpatternsandincreasedtolerancetodroughtstressinarabidopsis AT bohu expressionofahatl1anabatransportfactorgenefrompeanutisaffectedbyalteredmemorygeneexpressionpatternsandincreasedtolerancetodroughtstressinarabidopsis |