Development of a Wound-Healing Protocol for In Vitro Evaluation of Urothelial Cell Growth
Urethral healing is plagued by strictures, impacting quality of life and medical costs. Various growth factors (GFs) have shown promise as therapeutic approaches to improve healing, but there is no protocol for in vitro comparison between GFs. This study focuses the development of a biomimetic in vi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/6/4/64 |
| _version_ | 1797583632071655424 |
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| author | Christopher Foster Todd Jensen Christine Finck Courtney K. Rowe |
| author_facet | Christopher Foster Todd Jensen Christine Finck Courtney K. Rowe |
| author_sort | Christopher Foster |
| collection | DOAJ |
| description | Urethral healing is plagued by strictures, impacting quality of life and medical costs. Various growth factors (GFs) have shown promise as therapeutic approaches to improve healing, but there is no protocol for in vitro comparison between GFs. This study focuses the development of a biomimetic in vitro urothelial healing assay designed to mimic early in vivo healing, followed by an evaluation of urothelial cell growth in response to GFs. Methods: Wound-healing assays were developed with human urothelial cells and used to compared six GFs (EGF, FGF-2, IGF-1, PDGF, TGF-β1, and VEGF) at three concentrations (1 ng/mL, 10 ng/mL, and 100 ng/mL) over a 48 h period. A commercial GF-containing medium (EGF, TGF-α, KGF, and Extract P) and a GF-free medium were used as controls. Results: There was a statistically significant increase in cell growth for IGF-1 at 10 and 100 ng/mL compared to both controls (<i>p</i> < 0.05). There was a statistically significant increase in cell growth for EGF at all concentrations compared to the GF-free medium control (<i>p</i> < 0.05). Conclusion: This study shows the development of a clinically relevant wound-healing assay to evaluate urothelial cell growth. It is the first to compare GFs for future use in reconstructive techniques to improve urethral healing. |
| first_indexed | 2024-03-10T23:41:34Z |
| format | Article |
| id | doaj.art-83eac0ffa89e47f480bb030dfa698fe9 |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-10T23:41:34Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-83eac0ffa89e47f480bb030dfa698fe92023-11-19T02:26:13ZengMDPI AGMethods and Protocols2409-92792023-07-01646410.3390/mps6040064Development of a Wound-Healing Protocol for In Vitro Evaluation of Urothelial Cell GrowthChristopher Foster0Todd Jensen1Christine Finck2Courtney K. Rowe3Department of Pediatrics, University of Connecticut School of Medicine, Farmington, CT 06032, USADepartment of Pediatrics, University of Connecticut School of Medicine, Farmington, CT 06032, USADepartment of Pediatrics, University of Connecticut School of Medicine, Farmington, CT 06032, USADepartment of Pediatrics, University of Connecticut School of Medicine, Farmington, CT 06032, USAUrethral healing is plagued by strictures, impacting quality of life and medical costs. Various growth factors (GFs) have shown promise as therapeutic approaches to improve healing, but there is no protocol for in vitro comparison between GFs. This study focuses the development of a biomimetic in vitro urothelial healing assay designed to mimic early in vivo healing, followed by an evaluation of urothelial cell growth in response to GFs. Methods: Wound-healing assays were developed with human urothelial cells and used to compared six GFs (EGF, FGF-2, IGF-1, PDGF, TGF-β1, and VEGF) at three concentrations (1 ng/mL, 10 ng/mL, and 100 ng/mL) over a 48 h period. A commercial GF-containing medium (EGF, TGF-α, KGF, and Extract P) and a GF-free medium were used as controls. Results: There was a statistically significant increase in cell growth for IGF-1 at 10 and 100 ng/mL compared to both controls (<i>p</i> < 0.05). There was a statistically significant increase in cell growth for EGF at all concentrations compared to the GF-free medium control (<i>p</i> < 0.05). Conclusion: This study shows the development of a clinically relevant wound-healing assay to evaluate urothelial cell growth. It is the first to compare GFs for future use in reconstructive techniques to improve urethral healing.https://www.mdpi.com/2409-9279/6/4/64urethrawound healinggrowth factorregenerative medicine |
| spellingShingle | Christopher Foster Todd Jensen Christine Finck Courtney K. Rowe Development of a Wound-Healing Protocol for In Vitro Evaluation of Urothelial Cell Growth Methods and Protocols urethra wound healing growth factor regenerative medicine |
| title | Development of a Wound-Healing Protocol for In Vitro Evaluation of Urothelial Cell Growth |
| title_full | Development of a Wound-Healing Protocol for In Vitro Evaluation of Urothelial Cell Growth |
| title_fullStr | Development of a Wound-Healing Protocol for In Vitro Evaluation of Urothelial Cell Growth |
| title_full_unstemmed | Development of a Wound-Healing Protocol for In Vitro Evaluation of Urothelial Cell Growth |
| title_short | Development of a Wound-Healing Protocol for In Vitro Evaluation of Urothelial Cell Growth |
| title_sort | development of a wound healing protocol for in vitro evaluation of urothelial cell growth |
| topic | urethra wound healing growth factor regenerative medicine |
| url | https://www.mdpi.com/2409-9279/6/4/64 |
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