CELLULAR AND SUBCELLULAR LEVEL INVESTIGATION OF BIOLOGICAL OBJECTS BY MEANS OF FEMTOSECOND LASER OPTICAL TWEEZERS-SCALPEL
The aim of this work was developing of elements of the precise three-dimensional positioning technology of one or several micron and submicron size biological objects. Thereto a laboratory unit of hardware-software complex of optical femtosecond laser tweezers-scalpel was developed and constructed i...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2009-05-01
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| Series: | Vestnik Transplantologii i Iskusstvennyh Organov |
| Subjects: | |
| Online Access: | https://journal.transpl.ru/vtio/article/view/253 |
| _version_ | 1797872078256340992 |
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| author | M. M. Rakityansky M. B. Agranat S. I. Ashitkov M. N. Karagyaur D. M. Muhamedzhanova S. P. Domogatsky A. V. Ovchinnikov D. S. Sitnikov D. V. Stambolsky I. N. Shevelev |
| author_facet | M. M. Rakityansky M. B. Agranat S. I. Ashitkov M. N. Karagyaur D. M. Muhamedzhanova S. P. Domogatsky A. V. Ovchinnikov D. S. Sitnikov D. V. Stambolsky I. N. Shevelev |
| author_sort | M. M. Rakityansky |
| collection | DOAJ |
| description | The aim of this work was developing of elements of the precise three-dimensional positioning technology of one or several micron and submicron size biological objects. Thereto a laboratory unit of hardware-software complex of optical femtosecond laser tweezers-scalpel was developed and constructed in the Joint institute for high temperatures RAS using material resources of Russia. Experimental data concerning a maximal manipulation speed of CHO and cells, produced from mammalian spinal ganglia (using protocols for producing pure culture of Schwann cells) was received. Besides facts of interaction of laser radiation with intracellular structures that lead to unexpected behavior of cell in the zone of optical trap and change of maximal speed of cell manipulation were determined. |
| first_indexed | 2024-04-10T00:54:19Z |
| format | Article |
| id | doaj.art-83ec46e042a44fd5acc540ea33e3239f |
| institution | Directory Open Access Journal |
| issn | 1995-1191 |
| language | Russian |
| last_indexed | 2024-04-10T00:54:19Z |
| publishDate | 2009-05-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Vestnik Transplantologii i Iskusstvennyh Organov |
| spelling | doaj.art-83ec46e042a44fd5acc540ea33e3239f2023-03-13T10:37:19ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovVestnik Transplantologii i Iskusstvennyh Organov1995-11912009-05-0111310711310.15825/1995-1191-2009-3-107-113196CELLULAR AND SUBCELLULAR LEVEL INVESTIGATION OF BIOLOGICAL OBJECTS BY MEANS OF FEMTOSECOND LASER OPTICAL TWEEZERS-SCALPELM. M. Rakityansky0M. B. Agranat1S. I. Ashitkov2M. N. Karagyaur3D. M. Muhamedzhanova4S. P. Domogatsky5A. V. Ovchinnikov6D. S. Sitnikov7D. V. Stambolsky8I. N. Shevelev9Joint Institute for high Temperatures RAS, Moscow Burdenko Neurosurgery Institute, MoscowJoint Institute for high Temperatures RAS, MoscowJoint Institute for high Temperatures RAS, MoscowMoscow State University, Faculty of Fundamental MedicineMoscow State Medical Stomatological University, MoscowCardiology Research Center, MoscowJoint Institute for high Temperatures RAS, MoscowJoint Institute for high Temperatures RAS, MoscowCardiology Research Center, MoscowBurdenko Neurosurgery Institute, MoscowThe aim of this work was developing of elements of the precise three-dimensional positioning technology of one or several micron and submicron size biological objects. Thereto a laboratory unit of hardware-software complex of optical femtosecond laser tweezers-scalpel was developed and constructed in the Joint institute for high temperatures RAS using material resources of Russia. Experimental data concerning a maximal manipulation speed of CHO and cells, produced from mammalian spinal ganglia (using protocols for producing pure culture of Schwann cells) was received. Besides facts of interaction of laser radiation with intracellular structures that lead to unexpected behavior of cell in the zone of optical trap and change of maximal speed of cell manipulation were determined.https://journal.transpl.ru/vtio/article/view/253optical manipulationchinese hamster ovary cellsschwann cellsfemtosecond lasertissue engineering |
| spellingShingle | M. M. Rakityansky M. B. Agranat S. I. Ashitkov M. N. Karagyaur D. M. Muhamedzhanova S. P. Domogatsky A. V. Ovchinnikov D. S. Sitnikov D. V. Stambolsky I. N. Shevelev CELLULAR AND SUBCELLULAR LEVEL INVESTIGATION OF BIOLOGICAL OBJECTS BY MEANS OF FEMTOSECOND LASER OPTICAL TWEEZERS-SCALPEL Vestnik Transplantologii i Iskusstvennyh Organov optical manipulation chinese hamster ovary cells schwann cells femtosecond laser tissue engineering |
| title | CELLULAR AND SUBCELLULAR LEVEL INVESTIGATION OF BIOLOGICAL OBJECTS BY MEANS OF FEMTOSECOND LASER OPTICAL TWEEZERS-SCALPEL |
| title_full | CELLULAR AND SUBCELLULAR LEVEL INVESTIGATION OF BIOLOGICAL OBJECTS BY MEANS OF FEMTOSECOND LASER OPTICAL TWEEZERS-SCALPEL |
| title_fullStr | CELLULAR AND SUBCELLULAR LEVEL INVESTIGATION OF BIOLOGICAL OBJECTS BY MEANS OF FEMTOSECOND LASER OPTICAL TWEEZERS-SCALPEL |
| title_full_unstemmed | CELLULAR AND SUBCELLULAR LEVEL INVESTIGATION OF BIOLOGICAL OBJECTS BY MEANS OF FEMTOSECOND LASER OPTICAL TWEEZERS-SCALPEL |
| title_short | CELLULAR AND SUBCELLULAR LEVEL INVESTIGATION OF BIOLOGICAL OBJECTS BY MEANS OF FEMTOSECOND LASER OPTICAL TWEEZERS-SCALPEL |
| title_sort | cellular and subcellular level investigation of biological objects by means of femtosecond laser optical tweezers scalpel |
| topic | optical manipulation chinese hamster ovary cells schwann cells femtosecond laser tissue engineering |
| url | https://journal.transpl.ru/vtio/article/view/253 |
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