miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene Therapy
Gene therapy is widely used to treat incurable disorders and has become a routine procedure in clinical practice. Since viruses can exhibit specific tropisms, effectively penetrate the cell, and are easy to use, most gene therapy approaches are based on viral delivery of genetic material. However, v...
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MDPI AG
2022-11-01
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Series: | International Journal of Molecular Sciences |
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Online Access: | https://www.mdpi.com/1422-0067/23/23/14954 |
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author | Darya A. Savenkova Aelita-Luiza A. Makarova Igor K. Shalik Dmitry V. Yudkin |
author_facet | Darya A. Savenkova Aelita-Luiza A. Makarova Igor K. Shalik Dmitry V. Yudkin |
author_sort | Darya A. Savenkova |
collection | DOAJ |
description | Gene therapy is widely used to treat incurable disorders and has become a routine procedure in clinical practice. Since viruses can exhibit specific tropisms, effectively penetrate the cell, and are easy to use, most gene therapy approaches are based on viral delivery of genetic material. However, viral vectors have some disadvantages, such as immune response and cytotoxicity induced by a disturbance of cell metabolism, including miRNA pathways that are an important part of transcription regulation. Therefore, any viral-based gene therapy approach involves the evaluation of side effects and safety. It is possible for such effects to be caused either by the viral vectors themselves or by the delivered genetic material. Many gene therapy techniques use non-coding RNA delivery as an effective agent for gene expression regulation, with the risk of cellular miRNA pathways being affected due to the nature of the non-coding RNAs. This review describes the effect of viral vector entry and non-coding RNA delivery by these vectors on miRNA signaling pathways. |
first_indexed | 2024-03-09T17:45:34Z |
format | Article |
id | doaj.art-83efda3242b54b28a970c3b448a18870 |
institution | Directory Open Access Journal |
issn | 1661-6596 1422-0067 |
language | English |
last_indexed | 2024-03-09T17:45:34Z |
publishDate | 2022-11-01 |
publisher | MDPI AG |
record_format | Article |
series | International Journal of Molecular Sciences |
spelling | doaj.art-83efda3242b54b28a970c3b448a188702023-11-24T11:10:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231495410.3390/ijms232314954miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene TherapyDarya A. Savenkova0Aelita-Luiza A. Makarova1Igor K. Shalik2Dmitry V. Yudkin3State Research Center of Virology and Biotechnology “Vector,” Federal Service for Surveillance on Consumer Rights Protection and Human Well-being (FBRI SRC VB “Vector”, Rospotrebnadzor), Koltsovo 630559, RussiaState Research Center of Virology and Biotechnology “Vector,” Federal Service for Surveillance on Consumer Rights Protection and Human Well-being (FBRI SRC VB “Vector”, Rospotrebnadzor), Koltsovo 630559, RussiaState Research Center of Virology and Biotechnology “Vector,” Federal Service for Surveillance on Consumer Rights Protection and Human Well-being (FBRI SRC VB “Vector”, Rospotrebnadzor), Koltsovo 630559, RussiaState Research Center of Virology and Biotechnology “Vector,” Federal Service for Surveillance on Consumer Rights Protection and Human Well-being (FBRI SRC VB “Vector”, Rospotrebnadzor), Koltsovo 630559, RussiaGene therapy is widely used to treat incurable disorders and has become a routine procedure in clinical practice. Since viruses can exhibit specific tropisms, effectively penetrate the cell, and are easy to use, most gene therapy approaches are based on viral delivery of genetic material. However, viral vectors have some disadvantages, such as immune response and cytotoxicity induced by a disturbance of cell metabolism, including miRNA pathways that are an important part of transcription regulation. Therefore, any viral-based gene therapy approach involves the evaluation of side effects and safety. It is possible for such effects to be caused either by the viral vectors themselves or by the delivered genetic material. Many gene therapy techniques use non-coding RNA delivery as an effective agent for gene expression regulation, with the risk of cellular miRNA pathways being affected due to the nature of the non-coding RNAs. This review describes the effect of viral vector entry and non-coding RNA delivery by these vectors on miRNA signaling pathways.https://www.mdpi.com/1422-0067/23/23/14954miRNA pathwaysadeno-associated virusadenoviruslentivirusvectorgene therapy |
spellingShingle | Darya A. Savenkova Aelita-Luiza A. Makarova Igor K. Shalik Dmitry V. Yudkin miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene Therapy International Journal of Molecular Sciences miRNA pathways adeno-associated virus adenovirus lentivirus vector gene therapy |
title | miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene Therapy |
title_full | miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene Therapy |
title_fullStr | miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene Therapy |
title_full_unstemmed | miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene Therapy |
title_short | miRNA Pathway Alteration in Response to Non-Coding RNA Delivery in Viral Vector-Based Gene Therapy |
title_sort | mirna pathway alteration in response to non coding rna delivery in viral vector based gene therapy |
topic | miRNA pathways adeno-associated virus adenovirus lentivirus vector gene therapy |
url | https://www.mdpi.com/1422-0067/23/23/14954 |
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