Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis
Objective: The present study was an attempt to delineate the roles played by the neutrophil surface adhesion molecules and toll like receptors (TLRs) in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis. Materials and methods: Thirty six Karan Fries (KF) cows wer...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2016-06-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | http://www.scopemed.org/fulltextpdf.php?mno=222665 |
| _version_ | 1818932958437834752 |
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| author | Dilip Kumar Swain Mohar Singh Kushwah Ajay Kumar Dang |
| author_facet | Dilip Kumar Swain Mohar Singh Kushwah Ajay Kumar Dang |
| author_sort | Dilip Kumar Swain |
| collection | DOAJ |
| description | Objective: The present study was an attempt to delineate the roles played by the neutrophil surface adhesion molecules and toll like receptors (TLRs) in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis.
Materials and methods: Thirty six Karan Fries (KF) cows were categorized into three groups namely healthy (n=12), subclinical mastitis (SCM; n=12) and clinical mastitis (CM; n=12) after screening 146 cows. The grouping was done based on evaluation of collected milk samples by routine procedures like Californian Mastitis Test (CMT) scoring, microscopic counting of milk cells (SCC), bacterial culture of milk samples and observing gross changes in milk. Culture of milk and blood was done for the identification of Staphylococcus aureus subclinical and clinical mastitis.
Results: Healthy cows expressed significantly (P<0.05) higher L-selectin (CD62L) in both milk and blood neutrophils as compared to the animals suffering from SCM and CM; however, no significant difference was noticed between milk and blood neutrophils. Significant (P<0.05) increase in the expression of beta integrin (CD11b) was observed in the CM group of cows as compared to SCM and healthy cows. Similar trend in the expressions of TLR2 and TLR4 in both blood and milk neutrophils was observed in the CM cows as compared to the healthy and SCM cows. Milk neutrophils revealed a higher expression of TLR as compared to blood neutrophils.
Conclusion: Host elicits stage specific expression of surface adhesion molecules and TLR2 and TLR4 as dynamic host innate immune response against Staphylococcal mastitis. [J Adv Vet Anim Res 2016; 3(2.000): 99-105] |
| first_indexed | 2024-12-20T04:40:45Z |
| format | Article |
| id | doaj.art-83f05c0dd76c4b5f9c0c97125327a7c7 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-12-20T04:40:45Z |
| publishDate | 2016-06-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-83f05c0dd76c4b5f9c0c97125327a7c72022-12-21T19:53:08ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102016-06-01329910510.5455/javar.2016.c136222665Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitisDilip Kumar Swain0Mohar Singh Kushwah1Ajay Kumar Dang2Lactation and Immuno Physiology Laboratory, National Dairy Research Institute, Karnal 132 001, Haryana, India Lactation and Immuno Physiology Laboratory, National Dairy Research Institute, Karnal 132 001, Haryana, India Lactation and Immuno Physiology Laboratory, National Dairy Research Institute, Karnal 132 001, Haryana, IndiaObjective: The present study was an attempt to delineate the roles played by the neutrophil surface adhesion molecules and toll like receptors (TLRs) in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis. Materials and methods: Thirty six Karan Fries (KF) cows were categorized into three groups namely healthy (n=12), subclinical mastitis (SCM; n=12) and clinical mastitis (CM; n=12) after screening 146 cows. The grouping was done based on evaluation of collected milk samples by routine procedures like Californian Mastitis Test (CMT) scoring, microscopic counting of milk cells (SCC), bacterial culture of milk samples and observing gross changes in milk. Culture of milk and blood was done for the identification of Staphylococcus aureus subclinical and clinical mastitis. Results: Healthy cows expressed significantly (P<0.05) higher L-selectin (CD62L) in both milk and blood neutrophils as compared to the animals suffering from SCM and CM; however, no significant difference was noticed between milk and blood neutrophils. Significant (P<0.05) increase in the expression of beta integrin (CD11b) was observed in the CM group of cows as compared to SCM and healthy cows. Similar trend in the expressions of TLR2 and TLR4 in both blood and milk neutrophils was observed in the CM cows as compared to the healthy and SCM cows. Milk neutrophils revealed a higher expression of TLR as compared to blood neutrophils. Conclusion: Host elicits stage specific expression of surface adhesion molecules and TLR2 and TLR4 as dynamic host innate immune response against Staphylococcal mastitis. [J Adv Vet Anim Res 2016; 3(2.000): 99-105]http://www.scopemed.org/fulltextpdf.php?mno=222665CD11bCD62LCrossbred cowsMastitisNeutrophilsTLR |
| spellingShingle | Dilip Kumar Swain Mohar Singh Kushwah Ajay Kumar Dang Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis Journal of Advanced Veterinary and Animal Research CD11b CD62L Crossbred cows Mastitis Neutrophils TLR |
| title | Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis |
| title_full | Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis |
| title_fullStr | Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis |
| title_full_unstemmed | Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis |
| title_short | Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis |
| title_sort | neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from staphylococcus aureus subclinical and clinical mastitis |
| topic | CD11b CD62L Crossbred cows Mastitis Neutrophils TLR |
| url | http://www.scopemed.org/fulltextpdf.php?mno=222665 |
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