Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese population
Parasites are accountable for driving diversity within immune gene families. We identified and investigated regulatory single nucleotide polymorphisms (SNPs) in the promoter regions of the tumor necrosis factor receptor superfamily member 18 (TNFRSF18) gene by direct sequencing in a group of male Ga...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2011-05-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500006&lng=en&tlng=en |
| _version_ | 1819107119739174912 |
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| author | T.P. Velavan S. Bechlars X. Huang P.G. Kremsner J.F.J. Kun |
| author_facet | T.P. Velavan S. Bechlars X. Huang P.G. Kremsner J.F.J. Kun |
| author_sort | T.P. Velavan |
| collection | DOAJ |
| description | Parasites are accountable for driving diversity within immune gene families. We identified and investigated regulatory single nucleotide polymorphisms (SNPs) in the promoter regions of the tumor necrosis factor receptor superfamily member 18 (TNFRSF18) gene by direct sequencing in a group of male Gabonese individuals exposed to a wide array of parasitic diseases such as malaria, filariasis and schistosomiasis. Two new promoter variants were identified in 40 individuals. Both novel variants were heterozygous and were linked to SNP #rs3753344 (C/T), which has been described. One of the SNP variants (ss2080581728) was close to the general transcription factor site, the TATA box. We further validated these new promoter variants for their allelic gene expression using transient transfection assays. One new promoter variant with two base changes (C/T - ss2080581728/rs3753344) displayed an altered expression of the marker gene. Both novel variants remained less active at the non-induced state in comparison to the major allele. The allele frequencies observed in this study were consistent with data for other African populations. The detection and analysis of these human immune gene polymorphisms contribute to a better understanding of the interaction between host-parasite and expression of Treg activity. |
| first_indexed | 2024-12-22T02:48:58Z |
| format | Article |
| id | doaj.art-83f09a6ff33642e49b24cc69193c9251 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-22T02:48:58Z |
| publishDate | 2011-05-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-83f09a6ff33642e49b24cc69193c92512022-12-21T18:41:26ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2011-05-0144541842010.1590/S0100-879X2011000500006S0100-879X2011000500006Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese populationT.P. Velavan0S. Bechlars1X. Huang2P.G. Kremsner3J.F.J. Kun4Eberhard Karls Universität TübingenEberhard Karls Universität TübingenEberhard Karls Universität TübingenEberhard Karls Universität TübingenEberhard Karls Universität TübingenParasites are accountable for driving diversity within immune gene families. We identified and investigated regulatory single nucleotide polymorphisms (SNPs) in the promoter regions of the tumor necrosis factor receptor superfamily member 18 (TNFRSF18) gene by direct sequencing in a group of male Gabonese individuals exposed to a wide array of parasitic diseases such as malaria, filariasis and schistosomiasis. Two new promoter variants were identified in 40 individuals. Both novel variants were heterozygous and were linked to SNP #rs3753344 (C/T), which has been described. One of the SNP variants (ss2080581728) was close to the general transcription factor site, the TATA box. We further validated these new promoter variants for their allelic gene expression using transient transfection assays. One new promoter variant with two base changes (C/T - ss2080581728/rs3753344) displayed an altered expression of the marker gene. Both novel variants remained less active at the non-induced state in comparison to the major allele. The allele frequencies observed in this study were consistent with data for other African populations. The detection and analysis of these human immune gene polymorphisms contribute to a better understanding of the interaction between host-parasite and expression of Treg activity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500006&lng=en&tlng=enTumor necrosis factor receptor superfamilyPolymorphismTransfectionRegulatory T cells |
| spellingShingle | T.P. Velavan S. Bechlars X. Huang P.G. Kremsner J.F.J. Kun Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese population Brazilian Journal of Medical and Biological Research Tumor necrosis factor receptor superfamily Polymorphism Transfection Regulatory T cells |
| title | Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese population |
| title_full | Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese population |
| title_fullStr | Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese population |
| title_full_unstemmed | Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese population |
| title_short | Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese population |
| title_sort | novel regulatory snps in the promoter region of the tnfrsf18 gene in a gabonese population |
| topic | Tumor necrosis factor receptor superfamily Polymorphism Transfection Regulatory T cells |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500006&lng=en&tlng=en |
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