Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus
Abstract In this study, Pacific white shrimp (Penaeus vannamei), pond water, crab, Helice tridens, and live food samples were collected from farms in three provinces of Korea to investigate the presence of white spot syndrome virus (WSSV) and decapod hepanhamaparvovirus (DHPV). By polymerase chain r...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-02-01
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| Series: | Journal of the World Aquaculture Society |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jwas.13023 |
| _version_ | 1797306739205341184 |
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| author | Chorong Lee Hye Jin Jeon Bumkeun Kim Sangsu Suh Patharapol Piamsomboon Ji Hyung Kim Jee Eun Han |
| author_facet | Chorong Lee Hye Jin Jeon Bumkeun Kim Sangsu Suh Patharapol Piamsomboon Ji Hyung Kim Jee Eun Han |
| author_sort | Chorong Lee |
| collection | DOAJ |
| description | Abstract In this study, Pacific white shrimp (Penaeus vannamei), pond water, crab, Helice tridens, and live food samples were collected from farms in three provinces of Korea to investigate the presence of white spot syndrome virus (WSSV) and decapod hepanhamaparvovirus (DHPV). By polymerase chain reaction (PCR), DHPV was detected in all shrimp samples (83 of 83); among these, 41 samples were detected with WSSV. Amplicon sequencing and phylogenetic analyses indicated that all DHPV samples were classified as a novel genotype of DHPV. Additionally, the collected squid, polychaeta, and crab were also confirmed to be detected with novel genotypes of DHPV. By WSSV quantitative PCR, the average concentrations of viruses detected in shrimp and pond water samples were 2.58 × 107 copies/μL and 1.27 × 102 copies/μL, respectively. Simultaneous detection of WSSV and DHPV was observed in shrimp, pond water, and crab samples, and co‐infection with both viruses in shrimp could be demonstrated by histopathological analysis. Viral infection can result in significant economic losses in shrimp production. Therefore, precise monitoring and management of WSSV and DHPV prevalence in pond water, live food, and habitats are essential to preventing the spread of viral diseases in the Korean shrimp culture industry. |
| first_indexed | 2024-03-08T00:46:12Z |
| format | Article |
| id | doaj.art-83f75ea9d93548b08775781ec1db4598 |
| institution | Directory Open Access Journal |
| issn | 0893-8849 1749-7345 |
| language | English |
| last_indexed | 2024-03-08T00:46:12Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of the World Aquaculture Society |
| spelling | doaj.art-83f75ea9d93548b08775781ec1db45982024-02-15T10:37:45ZengWileyJournal of the World Aquaculture Society0893-88491749-73452024-02-0155137338510.1111/jwas.13023Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirusChorong Lee0Hye Jin Jeon1Bumkeun Kim2Sangsu Suh3Patharapol Piamsomboon4Ji Hyung Kim5Jee Eun Han6College of Veterinary Medicine Kyungpook National University Daegu South KoreaCollege of Veterinary Medicine Kyungpook National University Daegu South KoreaCollege of Veterinary Medicine Kyungpook National University Daegu South KoreaCollege of Veterinary Medicine Kyungpook National University Daegu South KoreaDepartment of Veterinary Medicine, Faculty of Veterinary Science Chulalongkorn University Bangkok ThailandDepartment of Food Science and Biotechnology Gachon University Seongnam South KoreaCollege of Veterinary Medicine Kyungpook National University Daegu South KoreaAbstract In this study, Pacific white shrimp (Penaeus vannamei), pond water, crab, Helice tridens, and live food samples were collected from farms in three provinces of Korea to investigate the presence of white spot syndrome virus (WSSV) and decapod hepanhamaparvovirus (DHPV). By polymerase chain reaction (PCR), DHPV was detected in all shrimp samples (83 of 83); among these, 41 samples were detected with WSSV. Amplicon sequencing and phylogenetic analyses indicated that all DHPV samples were classified as a novel genotype of DHPV. Additionally, the collected squid, polychaeta, and crab were also confirmed to be detected with novel genotypes of DHPV. By WSSV quantitative PCR, the average concentrations of viruses detected in shrimp and pond water samples were 2.58 × 107 copies/μL and 1.27 × 102 copies/μL, respectively. Simultaneous detection of WSSV and DHPV was observed in shrimp, pond water, and crab samples, and co‐infection with both viruses in shrimp could be demonstrated by histopathological analysis. Viral infection can result in significant economic losses in shrimp production. Therefore, precise monitoring and management of WSSV and DHPV prevalence in pond water, live food, and habitats are essential to preventing the spread of viral diseases in the Korean shrimp culture industry.https://doi.org/10.1111/jwas.13023co‐infectionsDHPVdiagnosismonitoringWSSV |
| spellingShingle | Chorong Lee Hye Jin Jeon Bumkeun Kim Sangsu Suh Patharapol Piamsomboon Ji Hyung Kim Jee Eun Han Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus Journal of the World Aquaculture Society co‐infections DHPV diagnosis monitoring WSSV |
| title | Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus |
| title_full | Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus |
| title_fullStr | Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus |
| title_full_unstemmed | Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus |
| title_short | Cultured Penaeus vannamei in Korea co‐infected with white spot syndrome virus and decapod hepanhamaparvovirus |
| title_sort | cultured penaeus vannamei in korea co infected with white spot syndrome virus and decapod hepanhamaparvovirus |
| topic | co‐infections DHPV diagnosis monitoring WSSV |
| url | https://doi.org/10.1111/jwas.13023 |
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