Fabrication of Biocompatible Electrospun Poly(ε-caprolactone)/Gelatin Nanofibers Loaded with <i>Pinus radiata</i> Bark Extracts for Wound Healing Applications
In this study, poly(ε-caprolactone) (PCL)/gelatin (GEL) electrospun nanofibers loaded with two different concentrations of <i>Pinus radiata</i> bark extracts (PEs) were fabricated via electrospinning for wound healing applications. The effects of incorporating PE into PCL/GEL electrospun...
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MDPI AG
2022-06-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/12/2331 |
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| author | Jessica Borges-Vilches Irem Unalan Katherina Fernández Aldo R. Boccaccini |
| author_facet | Jessica Borges-Vilches Irem Unalan Katherina Fernández Aldo R. Boccaccini |
| author_sort | Jessica Borges-Vilches |
| collection | DOAJ |
| description | In this study, poly(ε-caprolactone) (PCL)/gelatin (GEL) electrospun nanofibers loaded with two different concentrations of <i>Pinus radiata</i> bark extracts (PEs) were fabricated via electrospinning for wound healing applications. The effects of incorporating PE into PCL/GEL electrospun nanofibers were investigated regarding their physicochemical properties and in vitro biocompatibility. All electrospun nanofibers showed smooth, uniform, and bead-free surfaces. Their functional groups were detected by ATR-FTIR spectroscopy, and their total phenol content was measured by a Folin–Ciocalteu assay. With PE addition, the electrospun nanofibers exhibited an increase in their wettability and degradation rates over time and a decrease in their tensile stress values from 20 ± 4 to 8 ± 2 MPa for PCL/GEL and PCL/GEL/0.36%PE samples, respectively. PE was also released from the fibrous mats in a rather controlled fashion. The PCL/GEL/0.18%PE and PCL/GEL/0.36%PE electrospun nanofibers inhibited bacterial activity at around 6 ± 0.1% and 23 ± 0.3% against <i>E. coli</i> and 14 ± 0.1% and 18 ± 0.2% against <i>S. aureus</i> after 24 h incubation, respectively. In vitro cell studies showed that PE-loaded electrospun nanofibers enhanced HaCaT cell growth, attachment, and proliferation, favoring cell migration towards the scratch area in the wound healing assay and allowing a complete wound closure after 72 h treatment. These findings suggested that PE-loaded electrospun nanofibers are promising materials for antibiotic-free dressings for wound healing applications. |
| first_indexed | 2024-03-09T22:42:40Z |
| format | Article |
| id | doaj.art-83f9f54e85a94c65906f86568c80a143 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T22:42:40Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-83f9f54e85a94c65906f86568c80a1432023-11-23T18:36:09ZengMDPI AGPolymers2073-43602022-06-011412233110.3390/polym14122331Fabrication of Biocompatible Electrospun Poly(ε-caprolactone)/Gelatin Nanofibers Loaded with <i>Pinus radiata</i> Bark Extracts for Wound Healing ApplicationsJessica Borges-Vilches0Irem Unalan1Katherina Fernández2Aldo R. Boccaccini3Laboratory of Biomaterials, Department of Chemical Engineering, Faculty of Engineering, Universidad de Concepción, Concepción 4030000, ChileInstitute of Biomaterials, Department of Materials Science and Engineering, Friedrich-Alexander-University Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, GermanyLaboratory of Biomaterials, Department of Chemical Engineering, Faculty of Engineering, Universidad de Concepción, Concepción 4030000, ChileInstitute of Biomaterials, Department of Materials Science and Engineering, Friedrich-Alexander-University Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, GermanyIn this study, poly(ε-caprolactone) (PCL)/gelatin (GEL) electrospun nanofibers loaded with two different concentrations of <i>Pinus radiata</i> bark extracts (PEs) were fabricated via electrospinning for wound healing applications. The effects of incorporating PE into PCL/GEL electrospun nanofibers were investigated regarding their physicochemical properties and in vitro biocompatibility. All electrospun nanofibers showed smooth, uniform, and bead-free surfaces. Their functional groups were detected by ATR-FTIR spectroscopy, and their total phenol content was measured by a Folin–Ciocalteu assay. With PE addition, the electrospun nanofibers exhibited an increase in their wettability and degradation rates over time and a decrease in their tensile stress values from 20 ± 4 to 8 ± 2 MPa for PCL/GEL and PCL/GEL/0.36%PE samples, respectively. PE was also released from the fibrous mats in a rather controlled fashion. The PCL/GEL/0.18%PE and PCL/GEL/0.36%PE electrospun nanofibers inhibited bacterial activity at around 6 ± 0.1% and 23 ± 0.3% against <i>E. coli</i> and 14 ± 0.1% and 18 ± 0.2% against <i>S. aureus</i> after 24 h incubation, respectively. In vitro cell studies showed that PE-loaded electrospun nanofibers enhanced HaCaT cell growth, attachment, and proliferation, favoring cell migration towards the scratch area in the wound healing assay and allowing a complete wound closure after 72 h treatment. These findings suggested that PE-loaded electrospun nanofibers are promising materials for antibiotic-free dressings for wound healing applications.https://www.mdpi.com/2073-4360/14/12/2331poly(ε-caprolactone)gelatin<i>Pinus radiata</i> bark extractselectrospun nanofiberswound healing |
| spellingShingle | Jessica Borges-Vilches Irem Unalan Katherina Fernández Aldo R. Boccaccini Fabrication of Biocompatible Electrospun Poly(ε-caprolactone)/Gelatin Nanofibers Loaded with <i>Pinus radiata</i> Bark Extracts for Wound Healing Applications Polymers poly(ε-caprolactone) gelatin <i>Pinus radiata</i> bark extracts electrospun nanofibers wound healing |
| title | Fabrication of Biocompatible Electrospun Poly(ε-caprolactone)/Gelatin Nanofibers Loaded with <i>Pinus radiata</i> Bark Extracts for Wound Healing Applications |
| title_full | Fabrication of Biocompatible Electrospun Poly(ε-caprolactone)/Gelatin Nanofibers Loaded with <i>Pinus radiata</i> Bark Extracts for Wound Healing Applications |
| title_fullStr | Fabrication of Biocompatible Electrospun Poly(ε-caprolactone)/Gelatin Nanofibers Loaded with <i>Pinus radiata</i> Bark Extracts for Wound Healing Applications |
| title_full_unstemmed | Fabrication of Biocompatible Electrospun Poly(ε-caprolactone)/Gelatin Nanofibers Loaded with <i>Pinus radiata</i> Bark Extracts for Wound Healing Applications |
| title_short | Fabrication of Biocompatible Electrospun Poly(ε-caprolactone)/Gelatin Nanofibers Loaded with <i>Pinus radiata</i> Bark Extracts for Wound Healing Applications |
| title_sort | fabrication of biocompatible electrospun poly ε caprolactone gelatin nanofibers loaded with i pinus radiata i bark extracts for wound healing applications |
| topic | poly(ε-caprolactone) gelatin <i>Pinus radiata</i> bark extracts electrospun nanofibers wound healing |
| url | https://www.mdpi.com/2073-4360/14/12/2331 |
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