Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of <i>Globularia alypum</i> (L.) Leaves
<i>Globularia alypum</i> L. (GA) is a Mediterranean plant of the <i>Globulariaceae</i> family which is widely used in traditional Tunisian medicine. The main goal of this study was to evaluate the phytochemical composition, antioxidant, antibacterial, and antibiofilm activiti...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/1420-3049/28/10/4019 |
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| author | Sahar Nouir Amal Dbeibia Rim Bouhajeb Houda Haddad Amani Khélifa Lotfi Achour Mariem Ghardallou Amira Zaïri |
| author_facet | Sahar Nouir Amal Dbeibia Rim Bouhajeb Houda Haddad Amani Khélifa Lotfi Achour Mariem Ghardallou Amira Zaïri |
| author_sort | Sahar Nouir |
| collection | DOAJ |
| description | <i>Globularia alypum</i> L. (GA) is a Mediterranean plant of the <i>Globulariaceae</i> family which is widely used in traditional Tunisian medicine. The main goal of this study was to evaluate the phytochemical composition, antioxidant, antibacterial, and antibiofilm activities, and the antiproliferative potential of different extracts of this plant. The identification and the quantification of the different constituents of extracts were determined using gas chromatography–mass spectrometry (GC-MS). The antioxidant activities were evaluated using spectrophotometric methods and chemical tests. The antiproliferative study was based on the use of colorectal cancer SW620 cells, including an antibacterial assessment with the microdilution method and analysis of the antibiofilm effects via the crystal violet assay. All extracts presented several components, mainly sesquiterpenes, hydrocarbon, and oxygenated monoterpenes. The results revealed that the maceration extract had the most important antioxidant effect (IC<sub>50</sub> = 0.04 and 0.15 mg/mL), followed by the sonication extract (IC<sub>50</sub> = 0.18 and 0.28 mg/mL). However, the sonication extract demonstrated significant antiproliferative (IC<sub>50</sub> = 20 µg/mL), antibacterial (MIC = 6.25 mg/mLand MBC > 25 mg/mL), and antibiofilm (35.78% at 25 mg/mL) properties against <i>S. aureus</i>. The results achieved confirm the important role of this plant as a source of therapeutic activities. |
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| last_indexed | 2024-03-11T03:27:29Z |
| publishDate | 2023-05-01 |
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| series | Molecules |
| spelling | doaj.art-83fb70e86b8c477ba385bcb2ddf79f8a2023-11-18T02:38:06ZengMDPI AGMolecules1420-30492023-05-012810401910.3390/molecules28104019Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of <i>Globularia alypum</i> (L.) LeavesSahar Nouir0Amal Dbeibia1Rim Bouhajeb2Houda Haddad3Amani Khélifa4Lotfi Achour5Mariem Ghardallou6Amira Zaïri7Laboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, TunisiaLaboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy of Monastir, University of Monastir, Monastir 5000, TunisiaLaboratory of Bioactive Natural Substances and Biotechnology Research, Faculty of Dental Medicine of Monastir, University of Monastir, Monastir 5000, TunisiaLaboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, TunisiaLaboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, TunisiaHigh Institute of Biotechnology of Monastir, University of Monastir, Monastir 5000, TunisiaDepartment of Community Medicine, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, TunisiaLaboratory of Biochemistry, Faculty of Medicine of Sousse, University of Sousse, Sousse 4002, Tunisia<i>Globularia alypum</i> L. (GA) is a Mediterranean plant of the <i>Globulariaceae</i> family which is widely used in traditional Tunisian medicine. The main goal of this study was to evaluate the phytochemical composition, antioxidant, antibacterial, and antibiofilm activities, and the antiproliferative potential of different extracts of this plant. The identification and the quantification of the different constituents of extracts were determined using gas chromatography–mass spectrometry (GC-MS). The antioxidant activities were evaluated using spectrophotometric methods and chemical tests. The antiproliferative study was based on the use of colorectal cancer SW620 cells, including an antibacterial assessment with the microdilution method and analysis of the antibiofilm effects via the crystal violet assay. All extracts presented several components, mainly sesquiterpenes, hydrocarbon, and oxygenated monoterpenes. The results revealed that the maceration extract had the most important antioxidant effect (IC<sub>50</sub> = 0.04 and 0.15 mg/mL), followed by the sonication extract (IC<sub>50</sub> = 0.18 and 0.28 mg/mL). However, the sonication extract demonstrated significant antiproliferative (IC<sub>50</sub> = 20 µg/mL), antibacterial (MIC = 6.25 mg/mLand MBC > 25 mg/mL), and antibiofilm (35.78% at 25 mg/mL) properties against <i>S. aureus</i>. The results achieved confirm the important role of this plant as a source of therapeutic activities.https://www.mdpi.com/1420-3049/28/10/4019<i>Globularia alypum</i> (L.)phenolic compoundsantioxidant activityantiproliferative activityantibacterial activityantibiofilm activity |
| spellingShingle | Sahar Nouir Amal Dbeibia Rim Bouhajeb Houda Haddad Amani Khélifa Lotfi Achour Mariem Ghardallou Amira Zaïri Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of <i>Globularia alypum</i> (L.) Leaves Molecules <i>Globularia alypum</i> (L.) phenolic compounds antioxidant activity antiproliferative activity antibacterial activity antibiofilm activity |
| title | Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of <i>Globularia alypum</i> (L.) Leaves |
| title_full | Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of <i>Globularia alypum</i> (L.) Leaves |
| title_fullStr | Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of <i>Globularia alypum</i> (L.) Leaves |
| title_full_unstemmed | Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of <i>Globularia alypum</i> (L.) Leaves |
| title_short | Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of <i>Globularia alypum</i> (L.) Leaves |
| title_sort | phytochemical analysis and evaluation of the antioxidant antiproliferative antibacterial and antibiofilm effects of i globularia alypum i l leaves |
| topic | <i>Globularia alypum</i> (L.) phenolic compounds antioxidant activity antiproliferative activity antibacterial activity antibiofilm activity |
| url | https://www.mdpi.com/1420-3049/28/10/4019 |
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