Insecticidal Activity of <i>Hyoscyamus niger</i> L. on <i>Lucilia sericata</i> Causing Myiasis
Background: <i>Hyoscyamus niger</i> L. (Solanaceae) generally known as henbane, is commonly distributed in Europe and Asia. In Turkey, henbane seeds have been used in folk medicine to remove worms from the eyes. The present study aimed to investigate the insecticidal activity of <i>...
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MDPI AG
2020-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/5/655 |
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| author | Esra Küpeli Akkol Mert Ilhan Esma Kozan Fatma Tuğçe Gürağaç Dereli Mustafa Sak Eduardo Sobarzo-Sánchez |
| author_facet | Esra Küpeli Akkol Mert Ilhan Esma Kozan Fatma Tuğçe Gürağaç Dereli Mustafa Sak Eduardo Sobarzo-Sánchez |
| author_sort | Esra Küpeli Akkol |
| collection | DOAJ |
| description | Background: <i>Hyoscyamus niger</i> L. (Solanaceae) generally known as henbane, is commonly distributed in Europe and Asia. In Turkey, henbane seeds have been used in folk medicine to remove worms from the eyes. The present study aimed to investigate the insecticidal activity of <i>H. niger</i> seeds. Methods: <i>n</i>-hexane, ethyl acetate, methanol and alkaloid extracts were prepared from the seeds of the plant and their insecticidal activities on <i>Lucilia sericata</i> larvae were evaluated. EC<sub>50</sub> and EC<sub>90</sub> values of the alkaloid extract were calculated and morphological abnormalities were investigated. Results: Alkaloid extract prepared from the seeds of this plant displayed significant insecticidal activity. EC<sub>50</sub> values of <i>H. niger</i> seeds alkaloid extract were found to be 8.04, 8.49, 7.96 μg/mL against first, second and third instar, respectively. It was determined that malformations of larvae included damaged larvae with small size, contraction and weak cuticle. Furthermore, HPLC analysis was performed on alkaloid extract of <i>H. niger</i> seeds and main components of the extract were determined. It was determined that alkaloid extract mainly contain hyoscyamine and scopolamine. Conclusions: These results confirm the folkloric usage of the plant and suggest that the alkaloid content of the plant could be responsible for the insecticidal activity. |
| first_indexed | 2024-03-10T19:39:49Z |
| format | Article |
| id | doaj.art-83fcd46aba4c4be8a95f0c606e167f2b |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T19:39:49Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-83fcd46aba4c4be8a95f0c606e167f2b2023-11-20T01:24:32ZengMDPI AGPlants2223-77472020-05-019565510.3390/plants9050655Insecticidal Activity of <i>Hyoscyamus niger</i> L. on <i>Lucilia sericata</i> Causing MyiasisEsra Küpeli Akkol0Mert Ilhan1Esma Kozan2Fatma Tuğçe Gürağaç Dereli3Mustafa Sak4Eduardo Sobarzo-Sánchez5Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, Ankara 06330, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Van Yüzüncü Yıl University, Tuşba, Van 65080, TurkeyDepartment of Parasitology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Suleyman Demirel University, Isparta 32260, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, Ankara 06330, TurkeyInstituto de Investigación e Innovación en Salud, Facultad de Ciencias de la Salud, Universidad Central de Chile, Santiago 8330507, ChileBackground: <i>Hyoscyamus niger</i> L. (Solanaceae) generally known as henbane, is commonly distributed in Europe and Asia. In Turkey, henbane seeds have been used in folk medicine to remove worms from the eyes. The present study aimed to investigate the insecticidal activity of <i>H. niger</i> seeds. Methods: <i>n</i>-hexane, ethyl acetate, methanol and alkaloid extracts were prepared from the seeds of the plant and their insecticidal activities on <i>Lucilia sericata</i> larvae were evaluated. EC<sub>50</sub> and EC<sub>90</sub> values of the alkaloid extract were calculated and morphological abnormalities were investigated. Results: Alkaloid extract prepared from the seeds of this plant displayed significant insecticidal activity. EC<sub>50</sub> values of <i>H. niger</i> seeds alkaloid extract were found to be 8.04, 8.49, 7.96 μg/mL against first, second and third instar, respectively. It was determined that malformations of larvae included damaged larvae with small size, contraction and weak cuticle. Furthermore, HPLC analysis was performed on alkaloid extract of <i>H. niger</i> seeds and main components of the extract were determined. It was determined that alkaloid extract mainly contain hyoscyamine and scopolamine. Conclusions: These results confirm the folkloric usage of the plant and suggest that the alkaloid content of the plant could be responsible for the insecticidal activity.https://www.mdpi.com/2223-7747/9/5/655black henbane<i>Hyoscyamus niger</i><i>Lucilia sericata</i>myiasisSolanaceae |
| spellingShingle | Esra Küpeli Akkol Mert Ilhan Esma Kozan Fatma Tuğçe Gürağaç Dereli Mustafa Sak Eduardo Sobarzo-Sánchez Insecticidal Activity of <i>Hyoscyamus niger</i> L. on <i>Lucilia sericata</i> Causing Myiasis Plants black henbane <i>Hyoscyamus niger</i> <i>Lucilia sericata</i> myiasis Solanaceae |
| title | Insecticidal Activity of <i>Hyoscyamus niger</i> L. on <i>Lucilia sericata</i> Causing Myiasis |
| title_full | Insecticidal Activity of <i>Hyoscyamus niger</i> L. on <i>Lucilia sericata</i> Causing Myiasis |
| title_fullStr | Insecticidal Activity of <i>Hyoscyamus niger</i> L. on <i>Lucilia sericata</i> Causing Myiasis |
| title_full_unstemmed | Insecticidal Activity of <i>Hyoscyamus niger</i> L. on <i>Lucilia sericata</i> Causing Myiasis |
| title_short | Insecticidal Activity of <i>Hyoscyamus niger</i> L. on <i>Lucilia sericata</i> Causing Myiasis |
| title_sort | insecticidal activity of i hyoscyamus niger i l on i lucilia sericata i causing myiasis |
| topic | black henbane <i>Hyoscyamus niger</i> <i>Lucilia sericata</i> myiasis Solanaceae |
| url | https://www.mdpi.com/2223-7747/9/5/655 |
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