Community effects in regulation of translation
Certain forms of translational regulation, and translation itself, rely on long-range interactions between proteins bound to the different ends of mRNAs. A widespread assumption is that such interactions occur only in cis, between the two ends of a single transcript. However, certain translational r...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/10965 |
| _version_ | 1811228061222305792 |
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| author | Paul M Macdonald Matt Kanke Andrew Kenny |
| author_facet | Paul M Macdonald Matt Kanke Andrew Kenny |
| author_sort | Paul M Macdonald |
| collection | DOAJ |
| description | Certain forms of translational regulation, and translation itself, rely on long-range interactions between proteins bound to the different ends of mRNAs. A widespread assumption is that such interactions occur only in cis, between the two ends of a single transcript. However, certain translational regulatory defects of the Drosophila oskar (osk) mRNA can be rescued in trans. We proposed that inter-transcript interactions, promoted by assembly of the mRNAs in particles, allow regulatory elements to act in trans. Here we confirm predictions of that model and show that disruption of PTB-dependent particle assembly inhibits rescue in trans. Communication between transcripts is not limited to different osk mRNAs, as regulation imposed by cis-acting elements embedded in the osk mRNA spreads to gurken mRNA. We conclude that community effects exist in translational regulation. |
| first_indexed | 2024-04-12T09:52:10Z |
| format | Article |
| id | doaj.art-83fdb5f48599465d93681c8fc225d5a0 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:52:10Z |
| publishDate | 2016-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-83fdb5f48599465d93681c8fc225d5a02022-12-22T03:37:48ZengeLife Sciences Publications LtdeLife2050-084X2016-04-01510.7554/eLife.10965Community effects in regulation of translationPaul M Macdonald0https://orcid.org/0000-0001-5993-5343Matt Kanke1Andrew Kenny2Department of Molecular Biosciences, The University of Texas at Austin, Austin, United States; Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, United StatesDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, United States; Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, United StatesDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, United States; Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, United StatesCertain forms of translational regulation, and translation itself, rely on long-range interactions between proteins bound to the different ends of mRNAs. A widespread assumption is that such interactions occur only in cis, between the two ends of a single transcript. However, certain translational regulatory defects of the Drosophila oskar (osk) mRNA can be rescued in trans. We proposed that inter-transcript interactions, promoted by assembly of the mRNAs in particles, allow regulatory elements to act in trans. Here we confirm predictions of that model and show that disruption of PTB-dependent particle assembly inhibits rescue in trans. Communication between transcripts is not limited to different osk mRNAs, as regulation imposed by cis-acting elements embedded in the osk mRNA spreads to gurken mRNA. We conclude that community effects exist in translational regulation.https://elifesciences.org/articles/10965translationtranslational regulationregulation in transRNP |
| spellingShingle | Paul M Macdonald Matt Kanke Andrew Kenny Community effects in regulation of translation eLife translation translational regulation regulation in trans RNP |
| title | Community effects in regulation of translation |
| title_full | Community effects in regulation of translation |
| title_fullStr | Community effects in regulation of translation |
| title_full_unstemmed | Community effects in regulation of translation |
| title_short | Community effects in regulation of translation |
| title_sort | community effects in regulation of translation |
| topic | translation translational regulation regulation in trans RNP |
| url | https://elifesciences.org/articles/10965 |
| work_keys_str_mv | AT paulmmacdonald communityeffectsinregulationoftranslation AT mattkanke communityeffectsinregulationoftranslation AT andrewkenny communityeffectsinregulationoftranslation |