Community effects in regulation of translation
Certain forms of translational regulation, and translation itself, rely on long-range interactions between proteins bound to the different ends of mRNAs. A widespread assumption is that such interactions occur only in cis, between the two ends of a single transcript. However, certain translational r...
Main Authors: | , , |
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Format: | Article |
Language: | English |
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eLife Sciences Publications Ltd
2016-04-01
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Series: | eLife |
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Online Access: | https://elifesciences.org/articles/10965 |
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author | Paul M Macdonald Matt Kanke Andrew Kenny |
author_facet | Paul M Macdonald Matt Kanke Andrew Kenny |
author_sort | Paul M Macdonald |
collection | DOAJ |
description | Certain forms of translational regulation, and translation itself, rely on long-range interactions between proteins bound to the different ends of mRNAs. A widespread assumption is that such interactions occur only in cis, between the two ends of a single transcript. However, certain translational regulatory defects of the Drosophila oskar (osk) mRNA can be rescued in trans. We proposed that inter-transcript interactions, promoted by assembly of the mRNAs in particles, allow regulatory elements to act in trans. Here we confirm predictions of that model and show that disruption of PTB-dependent particle assembly inhibits rescue in trans. Communication between transcripts is not limited to different osk mRNAs, as regulation imposed by cis-acting elements embedded in the osk mRNA spreads to gurken mRNA. We conclude that community effects exist in translational regulation. |
first_indexed | 2024-04-12T09:52:10Z |
format | Article |
id | doaj.art-83fdb5f48599465d93681c8fc225d5a0 |
institution | Directory Open Access Journal |
issn | 2050-084X |
language | English |
last_indexed | 2024-04-12T09:52:10Z |
publishDate | 2016-04-01 |
publisher | eLife Sciences Publications Ltd |
record_format | Article |
series | eLife |
spelling | doaj.art-83fdb5f48599465d93681c8fc225d5a02022-12-22T03:37:48ZengeLife Sciences Publications LtdeLife2050-084X2016-04-01510.7554/eLife.10965Community effects in regulation of translationPaul M Macdonald0https://orcid.org/0000-0001-5993-5343Matt Kanke1Andrew Kenny2Department of Molecular Biosciences, The University of Texas at Austin, Austin, United States; Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, United StatesDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, United States; Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, United StatesDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, United States; Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, United StatesCertain forms of translational regulation, and translation itself, rely on long-range interactions between proteins bound to the different ends of mRNAs. A widespread assumption is that such interactions occur only in cis, between the two ends of a single transcript. However, certain translational regulatory defects of the Drosophila oskar (osk) mRNA can be rescued in trans. We proposed that inter-transcript interactions, promoted by assembly of the mRNAs in particles, allow regulatory elements to act in trans. Here we confirm predictions of that model and show that disruption of PTB-dependent particle assembly inhibits rescue in trans. Communication between transcripts is not limited to different osk mRNAs, as regulation imposed by cis-acting elements embedded in the osk mRNA spreads to gurken mRNA. We conclude that community effects exist in translational regulation.https://elifesciences.org/articles/10965translationtranslational regulationregulation in transRNP |
spellingShingle | Paul M Macdonald Matt Kanke Andrew Kenny Community effects in regulation of translation eLife translation translational regulation regulation in trans RNP |
title | Community effects in regulation of translation |
title_full | Community effects in regulation of translation |
title_fullStr | Community effects in regulation of translation |
title_full_unstemmed | Community effects in regulation of translation |
title_short | Community effects in regulation of translation |
title_sort | community effects in regulation of translation |
topic | translation translational regulation regulation in trans RNP |
url | https://elifesciences.org/articles/10965 |
work_keys_str_mv | AT paulmmacdonald communityeffectsinregulationoftranslation AT mattkanke communityeffectsinregulationoftranslation AT andrewkenny communityeffectsinregulationoftranslation |