Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature
Atomic force microscopy is an extremely versatile technique, featuring atomic-scale imaging resolution, and also offering the possibility to probe interaction forces down to few pN. Recently, this technique has been specialized to study the interaction between single living cells, one on the substra...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/21/4/1069 |
| _version_ | 1797415036553003008 |
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| author | Reinier Oropesa-Nuñez Andrea Mescola Massimo Vassalli Claudio Canale |
| author_facet | Reinier Oropesa-Nuñez Andrea Mescola Massimo Vassalli Claudio Canale |
| author_sort | Reinier Oropesa-Nuñez |
| collection | DOAJ |
| description | Atomic force microscopy is an extremely versatile technique, featuring atomic-scale imaging resolution, and also offering the possibility to probe interaction forces down to few pN. Recently, this technique has been specialized to study the interaction between single living cells, one on the substrate, and a second being adhered on the cantilever. Cell–cell force spectroscopy offers a unique tool to investigate in fine detail intra-cellular interactions, and it holds great promise to elucidate elusive phenomena in physiology and pathology. Here we present a systematic study of the effect of the main measurement parameters on cell–cell curves, showing the importance of controlling the experimental conditions. Moreover, a simple theoretical interpretation is proposed, based on the number of contacts formed between the two interacting cells. The results show that single cell–cell force spectroscopy experiments carry a wealth of information that can be exploited to understand the inner dynamics of the interaction of living cells at the molecular level. |
| first_indexed | 2024-03-09T05:43:17Z |
| format | Article |
| id | doaj.art-8403b7f598be4e769af6bfa3a30de07e |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T05:43:17Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-8403b7f598be4e769af6bfa3a30de07e2023-12-03T12:23:42ZengMDPI AGSensors1424-82202021-02-01214106910.3390/s21041069Impact of Experimental Parameters on Cell–Cell Force Spectroscopy SignatureReinier Oropesa-Nuñez0Andrea Mescola1Massimo Vassalli2Claudio Canale3Department of Materials Science and Engineering, Uppsala University, Ångströmlaboratoriet, Box 35, SE-751 03 Uppsala, SwedenCNR-Nanoscience Institute-S3, Via Campi 213/A, 41125 Modena, ItalyJames Watt School of Engineering, University of Glasgow, Glasgow G128LT, UKDepartment of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, ItalyAtomic force microscopy is an extremely versatile technique, featuring atomic-scale imaging resolution, and also offering the possibility to probe interaction forces down to few pN. Recently, this technique has been specialized to study the interaction between single living cells, one on the substrate, and a second being adhered on the cantilever. Cell–cell force spectroscopy offers a unique tool to investigate in fine detail intra-cellular interactions, and it holds great promise to elucidate elusive phenomena in physiology and pathology. Here we present a systematic study of the effect of the main measurement parameters on cell–cell curves, showing the importance of controlling the experimental conditions. Moreover, a simple theoretical interpretation is proposed, based on the number of contacts formed between the two interacting cells. The results show that single cell–cell force spectroscopy experiments carry a wealth of information that can be exploited to understand the inner dynamics of the interaction of living cells at the molecular level.https://www.mdpi.com/1424-8220/21/4/1069cell-cell interactionforce spectroscopyatomic force microscopycell mechanicsmechanobiology |
| spellingShingle | Reinier Oropesa-Nuñez Andrea Mescola Massimo Vassalli Claudio Canale Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature Sensors cell-cell interaction force spectroscopy atomic force microscopy cell mechanics mechanobiology |
| title | Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature |
| title_full | Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature |
| title_fullStr | Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature |
| title_full_unstemmed | Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature |
| title_short | Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature |
| title_sort | impact of experimental parameters on cell cell force spectroscopy signature |
| topic | cell-cell interaction force spectroscopy atomic force microscopy cell mechanics mechanobiology |
| url | https://www.mdpi.com/1424-8220/21/4/1069 |
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