Antioxidant and neuroprotective effects of gossypitrin, a flavonoid from Talipariti elatum, against chemical hypoxia-induced PC12 cell death
Context: Some flavonoids have been described as neuroprotectors. Gossypitrin (Gos) is a natural occurring flavonoid and the main bioactive substance from the flowers of Talipariti elatum Sw. (Majagua azul), traditionally used in Cuba as expectorant and antiasthmatic. Only few reports have documented...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2018-04-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol6/jppres17.314_6.2.72.pdf |
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| author | María A. Bécquer-Viart José González-Yaque Luis A. Fonseca-Fonseca Yanier Núñez-Figueredo Gilberto L. Pardo Andreu |
| author_facet | María A. Bécquer-Viart José González-Yaque Luis A. Fonseca-Fonseca Yanier Núñez-Figueredo Gilberto L. Pardo Andreu |
| author_sort | María A. Bécquer-Viart |
| collection | DOAJ |
| description | Context: Some flavonoids have been described as neuroprotectors. Gossypitrin (Gos) is a natural occurring flavonoid and the main bioactive substance from the flowers of Talipariti elatum Sw. (Majagua azul), traditionally used in Cuba as expectorant and antiasthmatic. Only few reports have documented its antioxidant properties.
Aims: To evaluate the antioxidant and cytoprotective effects of Gos against cyanide-induced oxidative stress and cell death in PC12 cells.
Methods: Gos effects on DPPH/ ABTS radical scavenging, ferric reducing power and lipid peroxidation were examined. The ischemia/reperfusion neuronal damage was produced by exposing PC12 cells to KCN in glucose-free medium. Gos was added to the incubation medium 30 min before chemical hypoxia induction. The neuroprotective potential of Gos was assessed by measuring cell viability by the MTT assay, the antioxidant enzymes SOD and CAT, and GSH and lipid peroxidation levels. Rutin (Rut), the well-known antioxidant, was used as a reference compound.
Results: Gos showed a potent intrinsic antioxidant capacity evidenced by low IC50 and EC50 values for DPPH/ABTS/malondialdehyde and ferric reducing power, respectively. Pre-treatment of PC12 cells with Gos, significantly increased their survival against KCN, restored the levels of GSH and the SOD and CAT enzymes activities, as well as reduced the level of lipid peroxidation. Its antioxidant effects were higher than those elicited by Rut.
Conclusions: The results show for the first time the neuroprotective potential of Gos against hypoxic cell damage, probably associated to its antioxidant effects. |
| first_indexed | 2024-12-13T18:52:12Z |
| format | Article |
| id | doaj.art-8406b58e62d040778535fc731dcc45b4 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-13T18:52:12Z |
| publishDate | 2018-04-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-8406b58e62d040778535fc731dcc45b42022-12-21T23:34:55ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502018-04-01627280Antioxidant and neuroprotective effects of gossypitrin, a flavonoid from Talipariti elatum, against chemical hypoxia-induced PC12 cell deathMaría A. Bécquer-Viart0José González-Yaque1Luis A. Fonseca-Fonseca2Yanier Núñez-Figueredo3Gilberto L. Pardo Andreu4Centro de Estudio para las Investigaciones y Evaluaciones Biológicas, Instituto de Farmacia y Alimentos, Universidad de La Habana, Calle 222, # 2317 e/23 y 31, La Coronela, La Lisa, CP 13600, La Habana, Cuba.Departamento de Farmacia, Instituto de Farmacia y Alimentos, Universidad de La Habana, Calle 222, # 2317 e/23 y 31, La Coronela, La Lisa, CP 13600, La Habana, Cuba.Centro de Investigación y Desarrollo de Medicamentos, Ave 26, No. 1605 Boyeros y Puentes Grandes, CP 10600, La Habana, Cuba.Centro de Investigación y Desarrollo de Medicamentos, Ave 26, No. 1605 Boyeros y Puentes Grandes, CP 10600, La Habana, Cuba.Centro de Estudio para las Investigaciones y Evaluaciones Biológicas, Instituto de Farmacia y Alimentos, Universidad de La Habana, Calle 222, # 2317 e/23 y 31, La Coronela, La Lisa, CP 13600, La Habana, Cuba.Context: Some flavonoids have been described as neuroprotectors. Gossypitrin (Gos) is a natural occurring flavonoid and the main bioactive substance from the flowers of Talipariti elatum Sw. (Majagua azul), traditionally used in Cuba as expectorant and antiasthmatic. Only few reports have documented its antioxidant properties. Aims: To evaluate the antioxidant and cytoprotective effects of Gos against cyanide-induced oxidative stress and cell death in PC12 cells. Methods: Gos effects on DPPH/ ABTS radical scavenging, ferric reducing power and lipid peroxidation were examined. The ischemia/reperfusion neuronal damage was produced by exposing PC12 cells to KCN in glucose-free medium. Gos was added to the incubation medium 30 min before chemical hypoxia induction. The neuroprotective potential of Gos was assessed by measuring cell viability by the MTT assay, the antioxidant enzymes SOD and CAT, and GSH and lipid peroxidation levels. Rutin (Rut), the well-known antioxidant, was used as a reference compound. Results: Gos showed a potent intrinsic antioxidant capacity evidenced by low IC50 and EC50 values for DPPH/ABTS/malondialdehyde and ferric reducing power, respectively. Pre-treatment of PC12 cells with Gos, significantly increased their survival against KCN, restored the levels of GSH and the SOD and CAT enzymes activities, as well as reduced the level of lipid peroxidation. Its antioxidant effects were higher than those elicited by Rut. Conclusions: The results show for the first time the neuroprotective potential of Gos against hypoxic cell damage, probably associated to its antioxidant effects.http://jppres.com/jppres/pdf/vol6/jppres17.314_6.2.72.pdfantioxidantgossypitrinhypoxiaischemianeuroprotectionPC12 |
| spellingShingle | María A. Bécquer-Viart José González-Yaque Luis A. Fonseca-Fonseca Yanier Núñez-Figueredo Gilberto L. Pardo Andreu Antioxidant and neuroprotective effects of gossypitrin, a flavonoid from Talipariti elatum, against chemical hypoxia-induced PC12 cell death Journal of Pharmacy & Pharmacognosy Research antioxidant gossypitrin hypoxia ischemia neuroprotection PC12 |
| title | Antioxidant and neuroprotective effects of gossypitrin, a flavonoid from Talipariti elatum, against chemical hypoxia-induced PC12 cell death |
| title_full | Antioxidant and neuroprotective effects of gossypitrin, a flavonoid from Talipariti elatum, against chemical hypoxia-induced PC12 cell death |
| title_fullStr | Antioxidant and neuroprotective effects of gossypitrin, a flavonoid from Talipariti elatum, against chemical hypoxia-induced PC12 cell death |
| title_full_unstemmed | Antioxidant and neuroprotective effects of gossypitrin, a flavonoid from Talipariti elatum, against chemical hypoxia-induced PC12 cell death |
| title_short | Antioxidant and neuroprotective effects of gossypitrin, a flavonoid from Talipariti elatum, against chemical hypoxia-induced PC12 cell death |
| title_sort | antioxidant and neuroprotective effects of gossypitrin a flavonoid from talipariti elatum against chemical hypoxia induced pc12 cell death |
| topic | antioxidant gossypitrin hypoxia ischemia neuroprotection PC12 |
| url | http://jppres.com/jppres/pdf/vol6/jppres17.314_6.2.72.pdf |
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