Optimized UV-Spectrophotometric Assay to Screen Bacterial Uricase Activity Using Whole Cell Suspension
Uricase catalyzes the conversion of uric acid into allantoin with concomitant reduction of molecular oxygen to hydrogen peroxide. In humans, uricase is not functional, thereby predisposing individuals to hyperuricemia, a metabolic disturbance associated with gout, chronic kidney disorders, and cardi...
Main Authors: | Benoit Pugin, Serafina Plüss, Denisa Mujezinovic, Rikke C. Nielsen, Christophe Lacroix |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2022-04-01
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Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.853735/full |
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