Evaluation of Genetic Variability in Four Nigerian Locally-Adapted Chicken Populations Using Major Histocompatibility Complex-Linked LEI0258 Microsatellite Marker
Major Histocompatibility Complex (MHC) is a group of genes that generally influence immune response in vertebrates, and it has been explored among different animal species in various countries. However, there is a paucity of information on its application in Nigerian locally-adapted chickens (NLAC)....
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Gorgan University of Agricultural Sciences and Natural Resources
2023-09-01
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| Series: | Poultry Science Journal |
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| Online Access: | https://psj.gau.ac.ir/article_6565_b7e9a2707a0d45fa45a6e2f028c01dec.pdf |
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| author | Opeyemi Oladejo Saidu Oseni Martina Kyallo, Jean-Baka Entfellner Noah Tor Christian Tiambo Roger Pelle |
| author_facet | Opeyemi Oladejo Saidu Oseni Martina Kyallo, Jean-Baka Entfellner Noah Tor Christian Tiambo Roger Pelle |
| author_sort | Opeyemi Oladejo |
| collection | DOAJ |
| description | Major Histocompatibility Complex (MHC) is a group of genes that generally influence immune response in vertebrates, and it has been explored among different animal species in various countries. However, there is a paucity of information on its application in Nigerian locally-adapted chickens (NLAC). This research investigated genetic polymorphism, allele variability, and genetic relationships using LEI0258 major histocompatibility complex-linked microsatellite marker among four NLAC populations: Fulani × Yoruba ecotypes, FUNNAB Alpha × Noiler breeds. Blood samples were randomly collected from 50 mature birds in each population and DNA was extracted and subsequently subjected to PCR, Sanger sequencing, and bioinformatic analysis. There were two variable numbers of tandem repeats (VNTRs), with 90% of the alleles containing only one R13 and varying numbers of the R12 motifs that ranged from 1 to 19. Additional polymorphism was revealed by the presence of five SNPs and three indels in the upstream and downstream regions of LEI0258. A total of 48 alleles were observed with sizes ranging from 188 to 530 base pairs while the allele frequencies within the populations ranged from 1.9 to 29.2%. However, only 17 out of the 48 alleles had corresponding MHC-B haplotypes. Haplotypes B2, B12, and B21 found in this study had been reported to confer resistance to infectious poultry diseases especially avian influenza in locally adapted chickens. There were high allelic variability and genetic polymorphisms observed via the atypical LEI0258 microsatellite in describing the MHC-B region. |
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| issn | 2345-6604 2345-6566 |
| language | English |
| last_indexed | 2024-03-11T21:50:35Z |
| publishDate | 2023-09-01 |
| publisher | Gorgan University of Agricultural Sciences and Natural Resources |
| record_format | Article |
| series | Poultry Science Journal |
| spelling | doaj.art-840ab6e14df44e45b26ec5766ba62d542023-09-26T09:01:51ZengGorgan University of Agricultural Sciences and Natural ResourcesPoultry Science Journal2345-66042345-65662023-09-0111218920110.22069/psj.2023.20536.18546565Evaluation of Genetic Variability in Four Nigerian Locally-Adapted Chicken Populations Using Major Histocompatibility Complex-Linked LEI0258 Microsatellite MarkerOpeyemi Oladejo0Saidu Oseni1Martina Kyallo,2Jean-Baka Entfellner3Noah Tor4Christian Tiambo5Roger Pelle6Animal Science and Fisheries Management, College of Agriculture, Engineering and Science, Bowen University, Iwo, Osun State, NigeriaDepartment of Animal Sciences, Obafemi Awolowo University, Ile-Ife, Osun State, NigeriaBiosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, KenyaBiosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, KenyaDepartment of Animal Breeding and Physiology, Federal University of Agriculture, Makurdi, Benue State, NigeriaBiosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, KenyaCentre for Tropical Livestock Genetics and Health (CTLGH) – ILRI, Nairobi KenyaMajor Histocompatibility Complex (MHC) is a group of genes that generally influence immune response in vertebrates, and it has been explored among different animal species in various countries. However, there is a paucity of information on its application in Nigerian locally-adapted chickens (NLAC). This research investigated genetic polymorphism, allele variability, and genetic relationships using LEI0258 major histocompatibility complex-linked microsatellite marker among four NLAC populations: Fulani × Yoruba ecotypes, FUNNAB Alpha × Noiler breeds. Blood samples were randomly collected from 50 mature birds in each population and DNA was extracted and subsequently subjected to PCR, Sanger sequencing, and bioinformatic analysis. There were two variable numbers of tandem repeats (VNTRs), with 90% of the alleles containing only one R13 and varying numbers of the R12 motifs that ranged from 1 to 19. Additional polymorphism was revealed by the presence of five SNPs and three indels in the upstream and downstream regions of LEI0258. A total of 48 alleles were observed with sizes ranging from 188 to 530 base pairs while the allele frequencies within the populations ranged from 1.9 to 29.2%. However, only 17 out of the 48 alleles had corresponding MHC-B haplotypes. Haplotypes B2, B12, and B21 found in this study had been reported to confer resistance to infectious poultry diseases especially avian influenza in locally adapted chickens. There were high allelic variability and genetic polymorphisms observed via the atypical LEI0258 microsatellite in describing the MHC-B region.https://psj.gau.ac.ir/article_6565_b7e9a2707a0d45fa45a6e2f028c01dec.pdfcharacterizationgenetic diversityimmune responselei0258 markernigerian locally-adapted chicken |
| spellingShingle | Opeyemi Oladejo Saidu Oseni Martina Kyallo, Jean-Baka Entfellner Noah Tor Christian Tiambo Roger Pelle Evaluation of Genetic Variability in Four Nigerian Locally-Adapted Chicken Populations Using Major Histocompatibility Complex-Linked LEI0258 Microsatellite Marker Poultry Science Journal characterization genetic diversity immune response lei0258 marker nigerian locally-adapted chicken |
| title | Evaluation of Genetic Variability in Four Nigerian Locally-Adapted Chicken Populations Using Major Histocompatibility Complex-Linked LEI0258 Microsatellite Marker |
| title_full | Evaluation of Genetic Variability in Four Nigerian Locally-Adapted Chicken Populations Using Major Histocompatibility Complex-Linked LEI0258 Microsatellite Marker |
| title_fullStr | Evaluation of Genetic Variability in Four Nigerian Locally-Adapted Chicken Populations Using Major Histocompatibility Complex-Linked LEI0258 Microsatellite Marker |
| title_full_unstemmed | Evaluation of Genetic Variability in Four Nigerian Locally-Adapted Chicken Populations Using Major Histocompatibility Complex-Linked LEI0258 Microsatellite Marker |
| title_short | Evaluation of Genetic Variability in Four Nigerian Locally-Adapted Chicken Populations Using Major Histocompatibility Complex-Linked LEI0258 Microsatellite Marker |
| title_sort | evaluation of genetic variability in four nigerian locally adapted chicken populations using major histocompatibility complex linked lei0258 microsatellite marker |
| topic | characterization genetic diversity immune response lei0258 marker nigerian locally-adapted chicken |
| url | https://psj.gau.ac.ir/article_6565_b7e9a2707a0d45fa45a6e2f028c01dec.pdf |
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