Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing <i>Penicillium piceum</i> and Investigation of the Novel β-Xylosidase Characteristics
A droplet-based microfluidic ultrahigh-throughput screening technology has been developed for the selection of high-β-xylosidase-producing <i>Penicillium piceum</i> W6 from the atmospheric and room-temperature plasma-mutated library of <i>P. piceum</i>. β-xylosidase hyperprod...
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MDPI AG
2022-03-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/4/325 |
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| author | Zhaokun Zhang Mingyue Ge Qi Guo Yi Jiang Wendi Jia Le Gao Jianhua Hu |
| author_facet | Zhaokun Zhang Mingyue Ge Qi Guo Yi Jiang Wendi Jia Le Gao Jianhua Hu |
| author_sort | Zhaokun Zhang |
| collection | DOAJ |
| description | A droplet-based microfluidic ultrahigh-throughput screening technology has been developed for the selection of high-β-xylosidase-producing <i>Penicillium piceum</i> W6 from the atmospheric and room-temperature plasma-mutated library of <i>P. piceum</i>. β-xylosidase hyperproducers filamentous fungi, <i>P. piceum</i> W6, exhibited an increase in β-xylosidase activity by 7.1-fold. A novel β-D-xylosidase was purified from the extracellular proteins of <i>P. piceum</i> W6 and designated as PpBXL. The optimal pH and temperature of PpBXL were 4.0 and 70 °C, respectively. PpBXL had high stability an acidic pH range of 3.0–5.0 and exhibited good thermostability with a thermal denaturation half-life of 10 days at 70 °C. Moreover, PpBXL showed the bifunctional activities of α-L-arabinofuranosidase and β-xylosidase. Supplementation with low-dose PpBXL (100 μg/g substrate) improved the yields of glucose and xylose generated from delignified biomass by 36–45%. The synergism between PpBXL and lignocellulolytic enzymes enhanced delignified biomass saccharification, increased the Xyl/Ara ratio, and decreased the strength of hydrogen bonds. |
| first_indexed | 2024-03-09T04:31:53Z |
| format | Article |
| id | doaj.art-840b3819e73b4c4ea5997716def43d38 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T04:31:53Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-840b3819e73b4c4ea5997716def43d382023-12-03T13:34:17ZengMDPI AGJournal of Fungi2309-608X2022-03-018432510.3390/jof8040325Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing <i>Penicillium piceum</i> and Investigation of the Novel β-Xylosidase CharacteristicsZhaokun Zhang0Mingyue Ge1Qi Guo2Yi Jiang3Wendi Jia4Le Gao5Jianhua Hu6School of Chemical Engineering, Inner Mongolia University of Technology, Hohhot 010051, ChinaTianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32, Xiqi Road, Tianjin Airport Economic Park, Tianjin 300308, ChinaTianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32, Xiqi Road, Tianjin Airport Economic Park, Tianjin 300308, ChinaTianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32, Xiqi Road, Tianjin Airport Economic Park, Tianjin 300308, ChinaTianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32, Xiqi Road, Tianjin Airport Economic Park, Tianjin 300308, ChinaTianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32, Xiqi Road, Tianjin Airport Economic Park, Tianjin 300308, ChinaSchool of Chemical Engineering, Inner Mongolia University of Technology, Hohhot 010051, ChinaA droplet-based microfluidic ultrahigh-throughput screening technology has been developed for the selection of high-β-xylosidase-producing <i>Penicillium piceum</i> W6 from the atmospheric and room-temperature plasma-mutated library of <i>P. piceum</i>. β-xylosidase hyperproducers filamentous fungi, <i>P. piceum</i> W6, exhibited an increase in β-xylosidase activity by 7.1-fold. A novel β-D-xylosidase was purified from the extracellular proteins of <i>P. piceum</i> W6 and designated as PpBXL. The optimal pH and temperature of PpBXL were 4.0 and 70 °C, respectively. PpBXL had high stability an acidic pH range of 3.0–5.0 and exhibited good thermostability with a thermal denaturation half-life of 10 days at 70 °C. Moreover, PpBXL showed the bifunctional activities of α-L-arabinofuranosidase and β-xylosidase. Supplementation with low-dose PpBXL (100 μg/g substrate) improved the yields of glucose and xylose generated from delignified biomass by 36–45%. The synergism between PpBXL and lignocellulolytic enzymes enhanced delignified biomass saccharification, increased the Xyl/Ara ratio, and decreased the strength of hydrogen bonds.https://www.mdpi.com/2309-608X/8/4/325ultrahigh-throughput screeningxylanolytic enzymesβ-D-xylosidasethermostabilityxylo-oligomersbiomass |
| spellingShingle | Zhaokun Zhang Mingyue Ge Qi Guo Yi Jiang Wendi Jia Le Gao Jianhua Hu Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing <i>Penicillium piceum</i> and Investigation of the Novel β-Xylosidase Characteristics Journal of Fungi ultrahigh-throughput screening xylanolytic enzymes β-D-xylosidase thermostability xylo-oligomers biomass |
| title | Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing <i>Penicillium piceum</i> and Investigation of the Novel β-Xylosidase Characteristics |
| title_full | Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing <i>Penicillium piceum</i> and Investigation of the Novel β-Xylosidase Characteristics |
| title_fullStr | Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing <i>Penicillium piceum</i> and Investigation of the Novel β-Xylosidase Characteristics |
| title_full_unstemmed | Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing <i>Penicillium piceum</i> and Investigation of the Novel β-Xylosidase Characteristics |
| title_short | Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing <i>Penicillium piceum</i> and Investigation of the Novel β-Xylosidase Characteristics |
| title_sort | ultrahigh throughput screening of high β xylosidase producing i penicillium piceum i and investigation of the novel β xylosidase characteristics |
| topic | ultrahigh-throughput screening xylanolytic enzymes β-D-xylosidase thermostability xylo-oligomers biomass |
| url | https://www.mdpi.com/2309-608X/8/4/325 |
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