The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju)
Abstract Background Diaphorina citri Kuwayama is an important citrus pest. It serves as the vector for the transmission of Candidatus Liberibacter asiaticus (CLas), which induced a destructive disease, Huanglongbing, and caused huge economic losses. During the interaction between insects and plants,...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09308-2 |
| _version_ | 1797769648252387328 |
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| author | Jinghua Dai Xueming Cai Luyang Liu Yanzheng Lin Yuting Huang Jintian Lin Benshui Shu |
| author_facet | Jinghua Dai Xueming Cai Luyang Liu Yanzheng Lin Yuting Huang Jintian Lin Benshui Shu |
| author_sort | Jinghua Dai |
| collection | DOAJ |
| description | Abstract Background Diaphorina citri Kuwayama is an important citrus pest. It serves as the vector for the transmission of Candidatus Liberibacter asiaticus (CLas), which induced a destructive disease, Huanglongbing, and caused huge economic losses. During the interaction between insects and plants, insects have evolved a series of mechanisms to adapt to various host plants. Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju) are the Rutaceae species from different genera that have been discovered as suitable hosts for D. citri adults. While the adaptation mechanism of this pest to these two host plants is unclear. Results In this study, RNA-seq and 16 S rDNA amplification sequencing were performed on the gut of D. citri adults reared on M. exotica and ‘Shatangju’ mandarin. RNA-seq results showed that a total of 964 differentially expressed genes were found in different gut groups with two host plant treatments. The impacted genes include those that encode ribosomal proteins, cathepsins, and mitochondrial respiratory chain complexes. According to 16 S rDNA sequencing, the compositions of the gut bacterial communities were altered by different treatments. The α and β diversity analyses confirmed that the host plant changes influenced the gut microbial diversity. The functional classification analysis by Tax4Fun revealed that 27 KEGG pathways, mostly those related to metabolism, including those for nucleotide metabolism, energy metabolism, metabolism of cofactors and vitamins, amino acid metabolism, carbohydrate metabolism, xenbiotics biodegradation and metabolism, lipid metabolism, and biosynthesis of other secondary metabolites, were significantly altered. Conclusion Our preliminary findings shed light on the connection between D. citri and host plants by showing that host plants alter the gene expression profiles and bacterial community composition of D. citri adults. |
| first_indexed | 2024-03-12T21:12:04Z |
| format | Article |
| id | doaj.art-840f797562da4cf883947140a60037f4 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-12T21:12:04Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-840f797562da4cf883947140a60037f42023-07-30T11:08:40ZengBMCBMC Genomics1471-21642023-07-0124111410.1186/s12864-023-09308-2The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju)Jinghua Dai0Xueming Cai1Luyang Liu2Yanzheng Lin3Yuting Huang4Jintian Lin5Benshui Shu6Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and EngineeringGuangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and EngineeringGuangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and EngineeringGuangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and EngineeringGuangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and EngineeringGuangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and EngineeringGuangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and EngineeringAbstract Background Diaphorina citri Kuwayama is an important citrus pest. It serves as the vector for the transmission of Candidatus Liberibacter asiaticus (CLas), which induced a destructive disease, Huanglongbing, and caused huge economic losses. During the interaction between insects and plants, insects have evolved a series of mechanisms to adapt to various host plants. Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju) are the Rutaceae species from different genera that have been discovered as suitable hosts for D. citri adults. While the adaptation mechanism of this pest to these two host plants is unclear. Results In this study, RNA-seq and 16 S rDNA amplification sequencing were performed on the gut of D. citri adults reared on M. exotica and ‘Shatangju’ mandarin. RNA-seq results showed that a total of 964 differentially expressed genes were found in different gut groups with two host plant treatments. The impacted genes include those that encode ribosomal proteins, cathepsins, and mitochondrial respiratory chain complexes. According to 16 S rDNA sequencing, the compositions of the gut bacterial communities were altered by different treatments. The α and β diversity analyses confirmed that the host plant changes influenced the gut microbial diversity. The functional classification analysis by Tax4Fun revealed that 27 KEGG pathways, mostly those related to metabolism, including those for nucleotide metabolism, energy metabolism, metabolism of cofactors and vitamins, amino acid metabolism, carbohydrate metabolism, xenbiotics biodegradation and metabolism, lipid metabolism, and biosynthesis of other secondary metabolites, were significantly altered. Conclusion Our preliminary findings shed light on the connection between D. citri and host plants by showing that host plants alter the gene expression profiles and bacterial community composition of D. citri adults.https://doi.org/10.1186/s12864-023-09308-2Diaphorina citriHost plantsRNA-seqDifferentially expressed genes16S rDNAGut bacterial community |
| spellingShingle | Jinghua Dai Xueming Cai Luyang Liu Yanzheng Lin Yuting Huang Jintian Lin Benshui Shu The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju) BMC Genomics Diaphorina citri Host plants RNA-seq Differentially expressed genes 16S rDNA Gut bacterial community |
| title | The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju) |
| title_full | The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju) |
| title_fullStr | The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju) |
| title_full_unstemmed | The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju) |
| title_short | The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju) |
| title_sort | comparison of gut gene expression and bacterial community in diaphorina citri hemiptera liviidae adults fed on murraya exotica and shatangju mandarin citrus reticulate cv shatangju |
| topic | Diaphorina citri Host plants RNA-seq Differentially expressed genes 16S rDNA Gut bacterial community |
| url | https://doi.org/10.1186/s12864-023-09308-2 |
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