Interfering lncRNA XIST inhibits the growth of salivary adenoid cystic carcinoma cells and improves cisplatin sensitivity by up-regulating miR-429
ObjectiveTo investigate the regulatory effect and mechanism of lncRNA XIST on salivary adenoid cystic carcinoma （SACC） cell growth and cisplatin （DDP） chemosensitivity. Methods Human SACC cell lines SACC-83, adenoid cystic carcinoma-2 （ACC-2） and normal submandibular gland cells HSG were cultured to...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of New Medicine
2022-07-01
|
| Series: | Xin yixue |
| Subjects: | |
| Online Access: | https://www.xinyixue.cn/fileup/0253-9802/PDF/1659601408208-267031118.pdf |
| _version_ | 1798016342836641792 |
|---|---|
| author | Zhang Xuan, Ma Licheng, Sun Longxiao, Zhang Lei |
| author_facet | Zhang Xuan, Ma Licheng, Sun Longxiao, Zhang Lei |
| author_sort | Zhang Xuan, Ma Licheng, Sun Longxiao, Zhang Lei |
| collection | DOAJ |
| description | ObjectiveTo investigate the regulatory effect and mechanism of lncRNA XIST on salivary adenoid cystic carcinoma （SACC） cell growth and cisplatin （DDP） chemosensitivity. Methods Human SACC cell lines SACC-83, adenoid cystic carcinoma-2 （ACC-2） and normal submandibular gland cells HSG were cultured to establish DDP-resistant SACC-83/DDP cells. The lncRNA XIST interfering plasmid and miR-429 mimic/inhibitor were respectively transfected into SACC-83 and SACC-83/DDP cells with Lipofectamine 2000. The mRNA expression levels of lncRNA XIST and miRNA（miR）-429 in cells were detected by RT-qPCR. CCK-8, clone formation assay, TUNEL assay and western blot were used to detect the effects of lncRNA XIST and miR-429 on the proliferation, apoptosis and DDP sensitivity of SACC cells, respectively. The targeted relationship between lncRNA XIST and miR-429 was verified by dual-luciferase assay. Results The lncRNA XIST was highly expressed in SACC cells. Interfering lncRNA XIST inhibited the proliferation and promoted the apoptosis of SACC-83 and SACC-83/DDP cells （all P < 0.001）. The expression of miR-429 was down-regulated in SACC cells, and lncRNA XIST targeted and negatively regulated the expression of miR-429. Down-regulation of miR-429 reversed the inhibitory effect of shRNA-XIST on the proliferation of SACC cells and its resistance to DDP （all P < 0.05）. Conclusion Interfering lncRNA XIST can inhibit the growth of SACC cells and improve the sensitivity of DDP by up-regulating miR-429. |
| first_indexed | 2024-04-11T15:49:13Z |
| format | Article |
| id | doaj.art-84141da9ec924094bed1bed081e05614 |
| institution | Directory Open Access Journal |
| issn | 0253-9802 |
| language | zho |
| last_indexed | 2024-04-11T15:49:13Z |
| publishDate | 2022-07-01 |
| publisher | Editorial Office of Journal of New Medicine |
| record_format | Article |
| series | Xin yixue |
| spelling | doaj.art-84141da9ec924094bed1bed081e056142022-12-22T04:15:25ZzhoEditorial Office of Journal of New MedicineXin yixue0253-98022022-07-0153751552310.3969/j.issn.0253-9802.2022.07.010Interfering lncRNA XIST inhibits the growth of salivary adenoid cystic carcinoma cells and improves cisplatin sensitivity by up-regulating miR-429Zhang Xuan, Ma Licheng, Sun Longxiao, Zhang Lei0△Department of Stomatology, the Second Hospital of Shandong University, Ji’nan 250033, ChinaObjectiveTo investigate the regulatory effect and mechanism of lncRNA XIST on salivary adenoid cystic carcinoma （SACC） cell growth and cisplatin （DDP） chemosensitivity. Methods Human SACC cell lines SACC-83, adenoid cystic carcinoma-2 （ACC-2） and normal submandibular gland cells HSG were cultured to establish DDP-resistant SACC-83/DDP cells. The lncRNA XIST interfering plasmid and miR-429 mimic/inhibitor were respectively transfected into SACC-83 and SACC-83/DDP cells with Lipofectamine 2000. The mRNA expression levels of lncRNA XIST and miRNA（miR）-429 in cells were detected by RT-qPCR. CCK-8, clone formation assay, TUNEL assay and western blot were used to detect the effects of lncRNA XIST and miR-429 on the proliferation, apoptosis and DDP sensitivity of SACC cells, respectively. The targeted relationship between lncRNA XIST and miR-429 was verified by dual-luciferase assay. Results The lncRNA XIST was highly expressed in SACC cells. Interfering lncRNA XIST inhibited the proliferation and promoted the apoptosis of SACC-83 and SACC-83/DDP cells （all P < 0.001）. The expression of miR-429 was down-regulated in SACC cells, and lncRNA XIST targeted and negatively regulated the expression of miR-429. Down-regulation of miR-429 reversed the inhibitory effect of shRNA-XIST on the proliferation of SACC cells and its resistance to DDP （all P < 0.05）. Conclusion Interfering lncRNA XIST can inhibit the growth of SACC cells and improve the sensitivity of DDP by up-regulating miR-429.https://www.xinyixue.cn/fileup/0253-9802/PDF/1659601408208-267031118.pdf|salivary adenoid cystic carcinoma|lncrna xist|cisplatin|drug resistance|proliferation|apoptosis |
| spellingShingle | Zhang Xuan, Ma Licheng, Sun Longxiao, Zhang Lei Interfering lncRNA XIST inhibits the growth of salivary adenoid cystic carcinoma cells and improves cisplatin sensitivity by up-regulating miR-429 Xin yixue |salivary adenoid cystic carcinoma|lncrna xist|cisplatin|drug resistance|proliferation|apoptosis |
| title | Interfering lncRNA XIST inhibits the growth of salivary adenoid cystic carcinoma cells and improves cisplatin sensitivity by up-regulating miR-429 |
| title_full | Interfering lncRNA XIST inhibits the growth of salivary adenoid cystic carcinoma cells and improves cisplatin sensitivity by up-regulating miR-429 |
| title_fullStr | Interfering lncRNA XIST inhibits the growth of salivary adenoid cystic carcinoma cells and improves cisplatin sensitivity by up-regulating miR-429 |
| title_full_unstemmed | Interfering lncRNA XIST inhibits the growth of salivary adenoid cystic carcinoma cells and improves cisplatin sensitivity by up-regulating miR-429 |
| title_short | Interfering lncRNA XIST inhibits the growth of salivary adenoid cystic carcinoma cells and improves cisplatin sensitivity by up-regulating miR-429 |
| title_sort | interfering lncrna xist inhibits the growth of salivary adenoid cystic carcinoma cells and improves cisplatin sensitivity by up regulating mir 429 |
| topic | |salivary adenoid cystic carcinoma|lncrna xist|cisplatin|drug resistance|proliferation|apoptosis |
| url | https://www.xinyixue.cn/fileup/0253-9802/PDF/1659601408208-267031118.pdf |
| work_keys_str_mv | AT zhangxuanmalichengsunlongxiaozhanglei interferinglncrnaxistinhibitsthegrowthofsalivaryadenoidcysticcarcinomacellsandimprovescisplatinsensitivitybyupregulatingmir429 |