Characterizing microRNA editing and mutation sites in Autism Spectrum Disorder
Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder whose pathogenesis is still unclear. MicroRNAs (miRNAs) are a kind of endogenous small non-coding RNAs that play important roles in the post-transcriptional regulation of genes. Recent researches show that miRNAs are edited in multiple...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Molecular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2022.1105278/full |
| _version_ | 1797946991249981440 |
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| author | Xingwang Wu Xingwang Wu Huaide Yang Han Lin Angbaji Suo Angbaji Suo Shuai Wu Shuai Wu Wenping Xie Wenping Xie Nan Zhou Shiyong Guo Shiyong Guo Hao Ding Guangchen Zhou Zhichao Qiu Zhichao Qiu Hong Shi Hong Shi Jun Yang Yun Zheng Yun Zheng Yun Zheng Yun Zheng |
| author_facet | Xingwang Wu Xingwang Wu Huaide Yang Han Lin Angbaji Suo Angbaji Suo Shuai Wu Shuai Wu Wenping Xie Wenping Xie Nan Zhou Shiyong Guo Shiyong Guo Hao Ding Guangchen Zhou Zhichao Qiu Zhichao Qiu Hong Shi Hong Shi Jun Yang Yun Zheng Yun Zheng Yun Zheng Yun Zheng |
| author_sort | Xingwang Wu |
| collection | DOAJ |
| description | Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder whose pathogenesis is still unclear. MicroRNAs (miRNAs) are a kind of endogenous small non-coding RNAs that play important roles in the post-transcriptional regulation of genes. Recent researches show that miRNAs are edited in multiple ways especially in central nervous systems. A-to-I editing of RNA catalyzed by Adenosine deaminases acting on RNA (ADARs) happens intensively in brain and is also noticed in other organs and tissues. Although miRNAs are widely edited in human brain, miRNA editing in ASD is still largely unexplored. In order to reveal the editing events of miRNAs in ASD, we analyzed 131 miRNA-seq samples from 8 different brain regions of ASD patients and normal controls. We identified 834 editing sites with significant editing levels, of which 70 sites showed significantly different editing levels in the superior frontal gyrus samples of ASD patients (ASD-SFG) when compared with those of control samples. The editing level of an A-to-I editing site in hsa-mir-376a-1 (hsa-mir-376a-1_9_A_g) in ASD-SFG is higher than that of normal controls, and the difference is exaggerated in individuals under 10 years. The increased expression of ADAR1 is consistent with the increased editing level of hsa-mir-376a-1_9_A_g in ASD-SFG samples compared to normal SFG samples. Furthermore, we verify that A-to-I edited hsa-mir-376a-5p directly represses GPR85 and NAPB, which may contribute to the abnormal neuronal development of ASD patients. These results provide new insights into the mechanism of ASD. |
| first_indexed | 2024-04-10T21:20:47Z |
| format | Article |
| id | doaj.art-8415617b422147d5b634fa51324243eb |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-04-10T21:20:47Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-8415617b422147d5b634fa51324243eb2023-01-20T05:40:59ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992023-01-011510.3389/fnmol.2022.11052781105278Characterizing microRNA editing and mutation sites in Autism Spectrum DisorderXingwang Wu0Xingwang Wu1Huaide Yang2Han Lin3Angbaji Suo4Angbaji Suo5Shuai Wu6Shuai Wu7Wenping Xie8Wenping Xie9Nan Zhou10Shiyong Guo11Shiyong Guo12Hao Ding13Guangchen Zhou14Zhichao Qiu15Zhichao Qiu16Hong Shi17Hong Shi18Jun Yang19Yun Zheng20Yun Zheng21Yun Zheng22Yun Zheng23State Key Laboratory of Primate Biomedical Research, Kunming University of Science and Technology, Kunming, Yunnan, ChinaInstitute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, ChinaFaculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, Yunnan, ChinaDepartment of Urology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, ChinaState Key Laboratory of Primate Biomedical Research, Kunming University of Science and Technology, Kunming, Yunnan, ChinaInstitute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, ChinaState Key Laboratory of Primate Biomedical Research, Kunming University of Science and Technology, Kunming, Yunnan, ChinaInstitute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, ChinaState Key Laboratory of Primate Biomedical Research, Kunming University of Science and Technology, Kunming, Yunnan, ChinaInstitute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, ChinaFaculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, Yunnan, ChinaState Key Laboratory of Primate Biomedical Research, Kunming University of Science and Technology, Kunming, Yunnan, ChinaInstitute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, ChinaFaculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, Yunnan, ChinaFaculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, Yunnan, ChinaState Key Laboratory of Primate Biomedical Research, Kunming University of Science and Technology, Kunming, Yunnan, ChinaInstitute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, ChinaState Key Laboratory of Primate Biomedical Research, Kunming University of Science and Technology, Kunming, Yunnan, ChinaInstitute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, ChinaSchool of Criminal Investigation, Yunnan Police College, Kunming, Yunnan, ChinaState Key Laboratory of Primate Biomedical Research, Kunming University of Science and Technology, Kunming, Yunnan, ChinaInstitute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, ChinaFaculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, Yunnan, ChinaCollege of Landscape and Horticulture, Yunnan Agricultural University, Kunming, Yunnan, ChinaAutism Spectrum Disorder (ASD) is a neurodevelopmental disorder whose pathogenesis is still unclear. MicroRNAs (miRNAs) are a kind of endogenous small non-coding RNAs that play important roles in the post-transcriptional regulation of genes. Recent researches show that miRNAs are edited in multiple ways especially in central nervous systems. A-to-I editing of RNA catalyzed by Adenosine deaminases acting on RNA (ADARs) happens intensively in brain and is also noticed in other organs and tissues. Although miRNAs are widely edited in human brain, miRNA editing in ASD is still largely unexplored. In order to reveal the editing events of miRNAs in ASD, we analyzed 131 miRNA-seq samples from 8 different brain regions of ASD patients and normal controls. We identified 834 editing sites with significant editing levels, of which 70 sites showed significantly different editing levels in the superior frontal gyrus samples of ASD patients (ASD-SFG) when compared with those of control samples. The editing level of an A-to-I editing site in hsa-mir-376a-1 (hsa-mir-376a-1_9_A_g) in ASD-SFG is higher than that of normal controls, and the difference is exaggerated in individuals under 10 years. The increased expression of ADAR1 is consistent with the increased editing level of hsa-mir-376a-1_9_A_g in ASD-SFG samples compared to normal SFG samples. Furthermore, we verify that A-to-I edited hsa-mir-376a-5p directly represses GPR85 and NAPB, which may contribute to the abnormal neuronal development of ASD patients. These results provide new insights into the mechanism of ASD.https://www.frontiersin.org/articles/10.3389/fnmol.2022.1105278/fullmiRNA editingAutism Spectrum Disorderhsa-miR-376a-5pGPR85NAPB |
| spellingShingle | Xingwang Wu Xingwang Wu Huaide Yang Han Lin Angbaji Suo Angbaji Suo Shuai Wu Shuai Wu Wenping Xie Wenping Xie Nan Zhou Shiyong Guo Shiyong Guo Hao Ding Guangchen Zhou Zhichao Qiu Zhichao Qiu Hong Shi Hong Shi Jun Yang Yun Zheng Yun Zheng Yun Zheng Yun Zheng Characterizing microRNA editing and mutation sites in Autism Spectrum Disorder Frontiers in Molecular Neuroscience miRNA editing Autism Spectrum Disorder hsa-miR-376a-5p GPR85 NAPB |
| title | Characterizing microRNA editing and mutation sites in Autism Spectrum Disorder |
| title_full | Characterizing microRNA editing and mutation sites in Autism Spectrum Disorder |
| title_fullStr | Characterizing microRNA editing and mutation sites in Autism Spectrum Disorder |
| title_full_unstemmed | Characterizing microRNA editing and mutation sites in Autism Spectrum Disorder |
| title_short | Characterizing microRNA editing and mutation sites in Autism Spectrum Disorder |
| title_sort | characterizing microrna editing and mutation sites in autism spectrum disorder |
| topic | miRNA editing Autism Spectrum Disorder hsa-miR-376a-5p GPR85 NAPB |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2022.1105278/full |
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