Genome-Wide QTL Mapping for Stripe Rust Resistance in Winter Wheat Pindong 34 Using a 90K SNP Array
Winter wheat cultivar Pindong 34 has both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust, which is caused by Puccinia striiformis f. sp. tritici (Pst). To map the quantitative trait loci (QTL) for stripe rust resistance, an F6−10 recombinant inbred line (RIL) population f...
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.932762/full |
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| author | Xinli Zhou Xin Li Dejun Han Suizhuang Yang Zhensheng Kang Runsheng Ren |
| author_facet | Xinli Zhou Xin Li Dejun Han Suizhuang Yang Zhensheng Kang Runsheng Ren |
| author_sort | Xinli Zhou |
| collection | DOAJ |
| description | Winter wheat cultivar Pindong 34 has both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust, which is caused by Puccinia striiformis f. sp. tritici (Pst). To map the quantitative trait loci (QTL) for stripe rust resistance, an F6−10 recombinant inbred line (RIL) population from a cross of Mingxian 169 × Pingdong 34 was phenotyped for stripe rust response over multiple years in fields under natural infection conditions and with selected Pst races under controlled greenhouse conditions, and genotyping was performed with a 90K single nucleotide polymorphism (SNP) array chip. Inclusive composite interval mapping (ICIM) identified 12 APR resistance QTLs and 3 ASR resistance QTLs. Among the 12 APR resistance QTLs, QYrpd.swust-1BL (explaining 9.24–13.33% of the phenotypic variation), QYrpd.swust-3AL.1 (11.41–14.80%), QYrpd.swust-3AL.2 (11.55–16.10%), QYrpd.swust-6BL (9.39–12.78%), QYrpd.swust-6DL (9.52–16.36%), QYrpd.swust-7AL (9.09–17.0%), and QYrpd.swust-7DL (8.87–11.38%) were more abundant than in the five tested environments and QYrpd.swust-1AS (11.05–12.72%), QYrpd.swust-1DL (9.81–13.05%), QYrpd.swust-2BL.1 (9.69–10.57%), QYrpd.swust-2BL.2 (10.36–12.97%), and QYrpd.swust-2BL.3 (9.54–13.15%) were significant in some of the tests. The three ASR resistance QTLs QYrpd.swust-2AS (9.69–13.58%), QYrpd.swust-2BL.4 (9.49–12.07%), and QYrpd.swust-7AS (16.16%) were detected based on the reactions in the seedlings tested with the CYR34 Pst race. Among the 15 QTLs detected in Pindong 34, the ASR resistance gene QYrpd.swust-7AS mapped on the short arm of chromosome 7A was likely similar to the previously reported QTL Yr61 in the region. The QTLs identified in the present study and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust. |
| first_indexed | 2024-04-13T11:42:54Z |
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| last_indexed | 2024-04-13T11:42:54Z |
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| spelling | doaj.art-8419b9d9123949c5931c8a876ae680282022-12-22T02:48:15ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.932762932762Genome-Wide QTL Mapping for Stripe Rust Resistance in Winter Wheat Pindong 34 Using a 90K SNP ArrayXinli Zhou0Xin Li1Dejun Han2Suizhuang Yang3Zhensheng Kang4Runsheng Ren5School of Life Sciences and Engineering, Wheat Research Institute, Southwest University of Science and Technology, Mianyang, ChinaSchool of Life Sciences and Engineering, Wheat Research Institute, Southwest University of Science and Technology, Mianyang, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A&F University, Xianyang, ChinaSchool of Life Sciences and Engineering, Wheat Research Institute, Southwest University of Science and Technology, Mianyang, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A&F University, Xianyang, ChinaExcellence and Innovation Center, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaWinter wheat cultivar Pindong 34 has both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust, which is caused by Puccinia striiformis f. sp. tritici (Pst). To map the quantitative trait loci (QTL) for stripe rust resistance, an F6−10 recombinant inbred line (RIL) population from a cross of Mingxian 169 × Pingdong 34 was phenotyped for stripe rust response over multiple years in fields under natural infection conditions and with selected Pst races under controlled greenhouse conditions, and genotyping was performed with a 90K single nucleotide polymorphism (SNP) array chip. Inclusive composite interval mapping (ICIM) identified 12 APR resistance QTLs and 3 ASR resistance QTLs. Among the 12 APR resistance QTLs, QYrpd.swust-1BL (explaining 9.24–13.33% of the phenotypic variation), QYrpd.swust-3AL.1 (11.41–14.80%), QYrpd.swust-3AL.2 (11.55–16.10%), QYrpd.swust-6BL (9.39–12.78%), QYrpd.swust-6DL (9.52–16.36%), QYrpd.swust-7AL (9.09–17.0%), and QYrpd.swust-7DL (8.87–11.38%) were more abundant than in the five tested environments and QYrpd.swust-1AS (11.05–12.72%), QYrpd.swust-1DL (9.81–13.05%), QYrpd.swust-2BL.1 (9.69–10.57%), QYrpd.swust-2BL.2 (10.36–12.97%), and QYrpd.swust-2BL.3 (9.54–13.15%) were significant in some of the tests. The three ASR resistance QTLs QYrpd.swust-2AS (9.69–13.58%), QYrpd.swust-2BL.4 (9.49–12.07%), and QYrpd.swust-7AS (16.16%) were detected based on the reactions in the seedlings tested with the CYR34 Pst race. Among the 15 QTLs detected in Pindong 34, the ASR resistance gene QYrpd.swust-7AS mapped on the short arm of chromosome 7A was likely similar to the previously reported QTL Yr61 in the region. The QTLs identified in the present study and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.https://www.frontiersin.org/articles/10.3389/fpls.2022.932762/fullwheatstripe rustQTL mappingresistance gene90K wheat SNP array |
| spellingShingle | Xinli Zhou Xin Li Dejun Han Suizhuang Yang Zhensheng Kang Runsheng Ren Genome-Wide QTL Mapping for Stripe Rust Resistance in Winter Wheat Pindong 34 Using a 90K SNP Array Frontiers in Plant Science wheat stripe rust QTL mapping resistance gene 90K wheat SNP array |
| title | Genome-Wide QTL Mapping for Stripe Rust Resistance in Winter Wheat Pindong 34 Using a 90K SNP Array |
| title_full | Genome-Wide QTL Mapping for Stripe Rust Resistance in Winter Wheat Pindong 34 Using a 90K SNP Array |
| title_fullStr | Genome-Wide QTL Mapping for Stripe Rust Resistance in Winter Wheat Pindong 34 Using a 90K SNP Array |
| title_full_unstemmed | Genome-Wide QTL Mapping for Stripe Rust Resistance in Winter Wheat Pindong 34 Using a 90K SNP Array |
| title_short | Genome-Wide QTL Mapping for Stripe Rust Resistance in Winter Wheat Pindong 34 Using a 90K SNP Array |
| title_sort | genome wide qtl mapping for stripe rust resistance in winter wheat pindong 34 using a 90k snp array |
| topic | wheat stripe rust QTL mapping resistance gene 90K wheat SNP array |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.932762/full |
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