L1CAM as an E-selectin Ligand in Colon Cancer
Metastasis is the main cause of death among colorectal cancer (CRC) patients. E-selectin and its carbohydrate ligands, including sialyl Lewis X (sLe<sup>X</sup>) antigen, are key players in the binding of circulating tumor cells to the endothelium, which is one of the major events leadin...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/21/8286 |
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| author | Fanny M. Deschepper Roberta Zoppi Martina Pirro Paul J. Hensbergen Fabio Dall’Olio Maximillianos Kotsias Richard A. Gardner Daniel I.R. Spencer Paula A. Videira |
| author_facet | Fanny M. Deschepper Roberta Zoppi Martina Pirro Paul J. Hensbergen Fabio Dall’Olio Maximillianos Kotsias Richard A. Gardner Daniel I.R. Spencer Paula A. Videira |
| author_sort | Fanny M. Deschepper |
| collection | DOAJ |
| description | Metastasis is the main cause of death among colorectal cancer (CRC) patients. E-selectin and its carbohydrate ligands, including sialyl Lewis X (sLe<sup>X</sup>) antigen, are key players in the binding of circulating tumor cells to the endothelium, which is one of the major events leading to organ invasion. Nevertheless, the identity of the glycoprotein scaffolds presenting these glycans in CRC remains unclear. In this study, we firstly have characterized the glycoengineered cell line SW620 transfected with the fucosyltransferase 6 (<i>FUT6</i>) coding for the α1,3-fucosyltransferase 6 (FUT6), which is the main enzyme responsible for the synthesis of sLe<sup>X</sup> in CRC. The SW620FUT6 cell line expressed high levels of sLe<sup>X</sup> antigen and E-selectin ligands. Moreover, it displayed increased migration ability. E-selectin ligand glycoproteins were isolated from the SW620FUT6 cell line, identified by mass spectrometry, and validated by flow cytometry and Western blot (WB). The most prominent E-selectin ligand we identified was the neural cell adhesion molecule L1 (L1CAM). Previous studies have shown association of L1CAM with metastasis in cancer, thus the novel role as E-selectin counter-receptor contributes to understand the molecular mechanism involving L1CAM in metastasis formation. |
| first_indexed | 2024-03-10T15:04:12Z |
| format | Article |
| id | doaj.art-841a05b44131422282dca4d17bbe24ef |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:04:12Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-841a05b44131422282dca4d17bbe24ef2023-11-20T19:54:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012121828610.3390/ijms21218286L1CAM as an E-selectin Ligand in Colon CancerFanny M. Deschepper0Roberta Zoppi1Martina Pirro2Paul J. Hensbergen3Fabio Dall’Olio4Maximillianos Kotsias5Richard A. Gardner6Daniel I.R. Spencer7Paula A. Videira8Unidade de Ciências Biomoleculares Aplicadas (UCIBIO), Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, PortugalUnidade de Ciências Biomoleculares Aplicadas (UCIBIO), Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, PortugalCenter for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The NetherlandsCenter for Proteomics and Metabolomics, Leiden University Medical Center, 2300 RC Leiden, The NetherlandsDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, 40138 Bologna, ItalyLudger Ltd., Culham Science Centre, Abingdon, Oxfordshire OX14 3EB, UKLudger Ltd., Culham Science Centre, Abingdon, Oxfordshire OX14 3EB, UKLudger Ltd., Culham Science Centre, Abingdon, Oxfordshire OX14 3EB, UKUnidade de Ciências Biomoleculares Aplicadas (UCIBIO), Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, PortugalMetastasis is the main cause of death among colorectal cancer (CRC) patients. E-selectin and its carbohydrate ligands, including sialyl Lewis X (sLe<sup>X</sup>) antigen, are key players in the binding of circulating tumor cells to the endothelium, which is one of the major events leading to organ invasion. Nevertheless, the identity of the glycoprotein scaffolds presenting these glycans in CRC remains unclear. In this study, we firstly have characterized the glycoengineered cell line SW620 transfected with the fucosyltransferase 6 (<i>FUT6</i>) coding for the α1,3-fucosyltransferase 6 (FUT6), which is the main enzyme responsible for the synthesis of sLe<sup>X</sup> in CRC. The SW620FUT6 cell line expressed high levels of sLe<sup>X</sup> antigen and E-selectin ligands. Moreover, it displayed increased migration ability. E-selectin ligand glycoproteins were isolated from the SW620FUT6 cell line, identified by mass spectrometry, and validated by flow cytometry and Western blot (WB). The most prominent E-selectin ligand we identified was the neural cell adhesion molecule L1 (L1CAM). Previous studies have shown association of L1CAM with metastasis in cancer, thus the novel role as E-selectin counter-receptor contributes to understand the molecular mechanism involving L1CAM in metastasis formation.https://www.mdpi.com/1422-0067/21/21/8286colorectal cancerE-selectin ligandL1CAMsialyl Lewis X antigen |
| spellingShingle | Fanny M. Deschepper Roberta Zoppi Martina Pirro Paul J. Hensbergen Fabio Dall’Olio Maximillianos Kotsias Richard A. Gardner Daniel I.R. Spencer Paula A. Videira L1CAM as an E-selectin Ligand in Colon Cancer International Journal of Molecular Sciences colorectal cancer E-selectin ligand L1CAM sialyl Lewis X antigen |
| title | L1CAM as an E-selectin Ligand in Colon Cancer |
| title_full | L1CAM as an E-selectin Ligand in Colon Cancer |
| title_fullStr | L1CAM as an E-selectin Ligand in Colon Cancer |
| title_full_unstemmed | L1CAM as an E-selectin Ligand in Colon Cancer |
| title_short | L1CAM as an E-selectin Ligand in Colon Cancer |
| title_sort | l1cam as an e selectin ligand in colon cancer |
| topic | colorectal cancer E-selectin ligand L1CAM sialyl Lewis X antigen |
| url | https://www.mdpi.com/1422-0067/21/21/8286 |
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