A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation Sequencing

A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation Sequencing

An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of <i>Orientia tsutsugamushi</i> 56-kDa...

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Bibliographic Details
Main Authors: Seungchan Cho, Jon C. Allison, Kkothanahreum Park, Jin Sun No, Seung-Ho Lee, Kyungmin Park, Jongwoo Kim, Terry A. Klein, Heung-Chul Kim, Won-Keun Kim, Jin-Won Song
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Pathogens
Subjects:
<i>Orientia tsutsugamushi</i>
scrub typhus
genetic identification
target enrichment next-generation sequencing
Online Access:https://www.mdpi.com/2076-0817/10/4/424
Description
Summary:An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of <i>Orientia tsutsugamushi</i> 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the <i>O. tsutsugamushi</i> aligned most closely with the Boryong strain.
ISSN:2076-0817

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