A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation Sequencing
An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of <i>Orientia tsutsugamushi</i> 56-kDa...
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MDPI AG
2021-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/4/424 |
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| author | Seungchan Cho Jon C. Allison Kkothanahreum Park Jin Sun No Seung-Ho Lee Kyungmin Park Jongwoo Kim Terry A. Klein Heung-Chul Kim Won-Keun Kim Jin-Won Song |
| author_facet | Seungchan Cho Jon C. Allison Kkothanahreum Park Jin Sun No Seung-Ho Lee Kyungmin Park Jongwoo Kim Terry A. Klein Heung-Chul Kim Won-Keun Kim Jin-Won Song |
| author_sort | Seungchan Cho |
| collection | DOAJ |
| description | An epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of <i>Orientia tsutsugamushi</i> 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the <i>O. tsutsugamushi</i> aligned most closely with the Boryong strain. |
| first_indexed | 2024-03-10T12:38:44Z |
| format | Article |
| id | doaj.art-841a2bb52ad34b09bb05ee1b5eded435 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T12:38:44Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-841a2bb52ad34b09bb05ee1b5eded4352023-11-21T14:01:30ZengMDPI AGPathogens2076-08172021-04-0110442410.3390/pathogens10040424A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation SequencingSeungchan Cho0Jon C. Allison1Kkothanahreum Park2Jin Sun No3Seung-Ho Lee4Kyungmin Park5Jongwoo Kim6Terry A. Klein7Heung-Chul Kim8Won-Keun Kim9Jin-Won Song10Department of Microbiology, College of Medicine, Korea University, Seoul 02841, KoreaForce Health Protection and Preventive Medicine, MEDDAC-Korea, 65th Medical Brigade, Unit 15281, APO AP 96251-5281, USADepartment of Microbiology, College of Medicine, Korea University, Seoul 02841, KoreaDepartment of Microbiology, College of Medicine, Korea University, Seoul 02841, KoreaDepartment of Microbiology, College of Medicine, Korea University, Seoul 02841, KoreaDepartment of Microbiology, College of Medicine, Korea University, Seoul 02841, KoreaDepartment of Microbiology, College of Medicine, Korea University, Seoul 02841, KoreaForce Health Protection and Preventive Medicine, MEDDAC-Korea, 65th Medical Brigade, Unit 15281, APO AP 96251-5281, USAForce Health Protection and Preventive Medicine, MEDDAC-Korea, 65th Medical Brigade, Unit 15281, APO AP 96251-5281, USADepartment of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, KoreaDepartment of Microbiology, College of Medicine, Korea University, Seoul 02841, KoreaAn epidemiological investigation was conducted for a scrub typhus case reported in a U.S. Forces Korea military patient in the Republic of Korea in November 2018. The patient had a fever, maculopapular rash, and an eschar. The full-length sequence of <i>Orientia tsutsugamushi</i> 56-kDa type-specific antigen (TSA) gene was obtained from eschar tissue by multiplex PCR-based Next Generation Sequencing for genetic identification. Based on the 56-kDa TSA gene, the <i>O. tsutsugamushi</i> aligned most closely with the Boryong strain.https://www.mdpi.com/2076-0817/10/4/424<i>Orientia tsutsugamushi</i>scrub typhusgenetic identificationtarget enrichment next-generation sequencing |
| spellingShingle | Seungchan Cho Jon C. Allison Kkothanahreum Park Jin Sun No Seung-Ho Lee Kyungmin Park Jongwoo Kim Terry A. Klein Heung-Chul Kim Won-Keun Kim Jin-Won Song A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation Sequencing Pathogens <i>Orientia tsutsugamushi</i> scrub typhus genetic identification target enrichment next-generation sequencing |
| title | A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation Sequencing |
| title_full | A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation Sequencing |
| title_fullStr | A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation Sequencing |
| title_full_unstemmed | A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation Sequencing |
| title_short | A Clinical Case of Scrub Typhus in the United States Forces Korea Patient with Eschar and Genetic Identification of <i>Orientia tsutsugamushi</i> Using Multiplex PCR-Based Next-Generation Sequencing |
| title_sort | clinical case of scrub typhus in the united states forces korea patient with eschar and genetic identification of i orientia tsutsugamushi i using multiplex pcr based next generation sequencing |
| topic | <i>Orientia tsutsugamushi</i> scrub typhus genetic identification target enrichment next-generation sequencing |
| url | https://www.mdpi.com/2076-0817/10/4/424 |
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