Effects of Dietary Calcium Propionate Supplementation on Hypothalamic Neuropeptide Messenger RNA Expression and Growth Performance in Finishing Rambouillet Lambs
The objective of this experiment was to evaluate the effects of feeding different levels concentrations of dietary calcium propionate (CaPr) on lambs’ growth performance; ruminal fermentation parameters; glucose–insulin concentration; and hypothalamic mRNA expression for neuropeptide Y (NPY), agouti...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2075-1729/11/6/566 |
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| author | Oswaldo Cifuentes-Lopez Héctor A. Lee-Rangel German D. Mendoza Pablo Delgado-Sanchez Luz Guerrero-Gonzalez Alfonso Chay-Canul Juan Manuel Pinos-Rodriguez Rogelio Flores-Ramírez José Alejandro Roque-Jiménez Alejandro E. Relling |
| author_facet | Oswaldo Cifuentes-Lopez Héctor A. Lee-Rangel German D. Mendoza Pablo Delgado-Sanchez Luz Guerrero-Gonzalez Alfonso Chay-Canul Juan Manuel Pinos-Rodriguez Rogelio Flores-Ramírez José Alejandro Roque-Jiménez Alejandro E. Relling |
| author_sort | Oswaldo Cifuentes-Lopez |
| collection | DOAJ |
| description | The objective of this experiment was to evaluate the effects of feeding different levels concentrations of dietary calcium propionate (CaPr) on lambs’ growth performance; ruminal fermentation parameters; glucose–insulin concentration; and hypothalamic mRNA expression for neuropeptide Y (NPY), agouti-related peptide (AgRP), and proopiomelanocortin (POMC). Thirty-two individually fed lambs were randomly assigned to four treatments: (1) control diet (0 g/kg of CaPr), (2) low CaPr, (30 g/kg dry matter (DM)), (3) medium CaPr, (35 g/kg DM), and (4) high CaPr (40 g/kg DM). After 42 days of feeding, lambs were slaughtered for collecting samples of the hypothalamus. Data were analyzed as a complete randomized design, and means were separated using linear and quadratic polynomial contrast. Growth performance was not affected (<i>p</i> ≥ 0.11) by dietary CaPr inclusion. The ruminal concentration of total volatile fatty acids (VFA) increased linearly (<i>p</i> = 0.04) as dietary CaPr increased. Likewise, a linear increase in plasma insulin concentration (<i>p</i> = 0.03) as dietary CaPr concentration increased. The relative mRNA expression of NPY exhibited a quadratic effect (<i>p</i> < 0.01), but there were significant differences in the mRNA expression of AgRP and POMC (<i>p</i> ≥ 0.10). Dietary calcium propionate did not improve lamb growth performance in lambs feed with only forage diets. Intake was not correlated with feed intake with mRNA expression of neuropeptides. |
| first_indexed | 2024-03-10T10:22:34Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T10:22:34Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-841c78765dc7451abcb95422cb144fb72023-11-22T00:19:42ZengMDPI AGLife2075-17292021-06-0111656610.3390/life11060566Effects of Dietary Calcium Propionate Supplementation on Hypothalamic Neuropeptide Messenger RNA Expression and Growth Performance in Finishing Rambouillet LambsOswaldo Cifuentes-Lopez0Héctor A. Lee-Rangel1German D. Mendoza2Pablo Delgado-Sanchez3Luz Guerrero-Gonzalez4Alfonso Chay-Canul5Juan Manuel Pinos-Rodriguez6Rogelio Flores-Ramírez7José Alejandro Roque-Jiménez8Alejandro E. Relling9Facultad de Agronomía y Veterinaria, Centro de Biociencias, Universidad Autónoma de San Luis Potosí, San Luis Potosí 78321, MexicoFacultad de Agronomía y Veterinaria, Centro de Biociencias, Universidad Autónoma de San Luis Potosí, San Luis Potosí 78321, MexicoDepartamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana Xochimilco, Ciudad de México 04970, MexicoFacultad de Agronomía y Veterinaria, Centro de Biociencias, Universidad Autónoma de San Luis Potosí, San Luis Potosí 78321, MexicoFacultad de Agronomía y Veterinaria, Centro de Biociencias, Universidad Autónoma de San Luis Potosí, San Luis Potosí 78321, MexicoDivisión Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Tabasco 86040, MexicoFacultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Veracruz 91710, MexicoCONACYT, Coordinación Para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), San Luis Potosi 78210, MexicoFacultad de Agronomía y Veterinaria, Centro de Biociencias, Universidad Autónoma de San Luis Potosí, San Luis Potosí 78321, MexicoDepartment of Animal Sciences, The Ohio State University, OARDC, Wooster, OH 44691, USAThe objective of this experiment was to evaluate the effects of feeding different levels concentrations of dietary calcium propionate (CaPr) on lambs’ growth performance; ruminal fermentation parameters; glucose–insulin concentration; and hypothalamic mRNA expression for neuropeptide Y (NPY), agouti-related peptide (AgRP), and proopiomelanocortin (POMC). Thirty-two individually fed lambs were randomly assigned to four treatments: (1) control diet (0 g/kg of CaPr), (2) low CaPr, (30 g/kg dry matter (DM)), (3) medium CaPr, (35 g/kg DM), and (4) high CaPr (40 g/kg DM). After 42 days of feeding, lambs were slaughtered for collecting samples of the hypothalamus. Data were analyzed as a complete randomized design, and means were separated using linear and quadratic polynomial contrast. Growth performance was not affected (<i>p</i> ≥ 0.11) by dietary CaPr inclusion. The ruminal concentration of total volatile fatty acids (VFA) increased linearly (<i>p</i> = 0.04) as dietary CaPr increased. Likewise, a linear increase in plasma insulin concentration (<i>p</i> = 0.03) as dietary CaPr concentration increased. The relative mRNA expression of NPY exhibited a quadratic effect (<i>p</i> < 0.01), but there were significant differences in the mRNA expression of AgRP and POMC (<i>p</i> ≥ 0.10). Dietary calcium propionate did not improve lamb growth performance in lambs feed with only forage diets. Intake was not correlated with feed intake with mRNA expression of neuropeptides.https://www.mdpi.com/2075-1729/11/6/566agouti-related peptidecalcium propionateintakeneuropeptide YproopiomelanocortinRambouillet sheep |
| spellingShingle | Oswaldo Cifuentes-Lopez Héctor A. Lee-Rangel German D. Mendoza Pablo Delgado-Sanchez Luz Guerrero-Gonzalez Alfonso Chay-Canul Juan Manuel Pinos-Rodriguez Rogelio Flores-Ramírez José Alejandro Roque-Jiménez Alejandro E. Relling Effects of Dietary Calcium Propionate Supplementation on Hypothalamic Neuropeptide Messenger RNA Expression and Growth Performance in Finishing Rambouillet Lambs Life agouti-related peptide calcium propionate intake neuropeptide Y proopiomelanocortin Rambouillet sheep |
| title | Effects of Dietary Calcium Propionate Supplementation on Hypothalamic Neuropeptide Messenger RNA Expression and Growth Performance in Finishing Rambouillet Lambs |
| title_full | Effects of Dietary Calcium Propionate Supplementation on Hypothalamic Neuropeptide Messenger RNA Expression and Growth Performance in Finishing Rambouillet Lambs |
| title_fullStr | Effects of Dietary Calcium Propionate Supplementation on Hypothalamic Neuropeptide Messenger RNA Expression and Growth Performance in Finishing Rambouillet Lambs |
| title_full_unstemmed | Effects of Dietary Calcium Propionate Supplementation on Hypothalamic Neuropeptide Messenger RNA Expression and Growth Performance in Finishing Rambouillet Lambs |
| title_short | Effects of Dietary Calcium Propionate Supplementation on Hypothalamic Neuropeptide Messenger RNA Expression and Growth Performance in Finishing Rambouillet Lambs |
| title_sort | effects of dietary calcium propionate supplementation on hypothalamic neuropeptide messenger rna expression and growth performance in finishing rambouillet lambs |
| topic | agouti-related peptide calcium propionate intake neuropeptide Y proopiomelanocortin Rambouillet sheep |
| url | https://www.mdpi.com/2075-1729/11/6/566 |
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