pH Distribution along Growing Fungal Hyphae at Microscale
Creating unique microenvironments, hyphal surfaces and their surroundings allow for spatially distinct microbial interactions and functions at the microscale. Using a microfluidic system and pH-sensitive whole-cell bioreporters (<i>Synechocystis</i> sp. PCC6803) attached to hyphae, we sp...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/6/599 |
| _version_ | 1797485631717244928 |
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| author | Bi-Jing Xiong Claire E. Stanley Christian Dusny Dietmar Schlosser Hauke Harms Lukas Y. Wick |
| author_facet | Bi-Jing Xiong Claire E. Stanley Christian Dusny Dietmar Schlosser Hauke Harms Lukas Y. Wick |
| author_sort | Bi-Jing Xiong |
| collection | DOAJ |
| description | Creating unique microenvironments, hyphal surfaces and their surroundings allow for spatially distinct microbial interactions and functions at the microscale. Using a microfluidic system and pH-sensitive whole-cell bioreporters (<i>Synechocystis</i> sp. PCC6803) attached to hyphae, we spatially resolved the pH along surfaces of growing hyphae of the basidiomycete <i>Coprinopsis cinerea</i>. Time-lapse microscopy analysis of ratiometric fluorescence signals of >2400 individual bioreporters revealed an overall pH drop from 6.3 ± 0.4 (<i>n</i> = 2441) to 5.0 ± 0.3 (<i>n</i> = 2497) within 7 h after pH bioreporter loading to hyphal surfaces. The pH along hyphal surfaces varied significantly (<i>p</i> < 0.05), with pH at hyphal tips being on average ~0.8 pH units lower than at more mature hyphal parts near the entrance of the microfluidic observation chamber. Our data represent the first dynamic in vitro analysis of surface pH along growing hyphae at the micrometre scale. Such knowledge may improve our understanding of spatial, pH-dependent hyphal processes, such as the degradation of organic matter or mineral weathering. |
| first_indexed | 2024-03-09T23:22:34Z |
| format | Article |
| id | doaj.art-841ca2af652c4147be95d6bb6f0f477b |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T23:22:34Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-841ca2af652c4147be95d6bb6f0f477b2023-11-23T17:24:47ZengMDPI AGJournal of Fungi2309-608X2022-06-018659910.3390/jof8060599pH Distribution along Growing Fungal Hyphae at MicroscaleBi-Jing Xiong0Claire E. Stanley1Christian Dusny2Dietmar Schlosser3Hauke Harms4Lukas Y. Wick5Helmholtz Centre for Environmental Research-UFZ, Department of Environmental Microbiology, Permoserstraβe 15, 04318 Leipzig, GermanyDepartment of Bioengineering, Imperial College of London, South Kensington Campus, London SW7 2AZ, UKHelmholtz Centre for Environmental Research-UFZ, Department of Solar Materials, Permoserstraβe 15, 04318 Leipzig, GermanyHelmholtz Centre for Environmental Research-UFZ, Department of Environmental Microbiology, Permoserstraβe 15, 04318 Leipzig, GermanyHelmholtz Centre for Environmental Research-UFZ, Department of Environmental Microbiology, Permoserstraβe 15, 04318 Leipzig, GermanyHelmholtz Centre for Environmental Research-UFZ, Department of Environmental Microbiology, Permoserstraβe 15, 04318 Leipzig, GermanyCreating unique microenvironments, hyphal surfaces and their surroundings allow for spatially distinct microbial interactions and functions at the microscale. Using a microfluidic system and pH-sensitive whole-cell bioreporters (<i>Synechocystis</i> sp. PCC6803) attached to hyphae, we spatially resolved the pH along surfaces of growing hyphae of the basidiomycete <i>Coprinopsis cinerea</i>. Time-lapse microscopy analysis of ratiometric fluorescence signals of >2400 individual bioreporters revealed an overall pH drop from 6.3 ± 0.4 (<i>n</i> = 2441) to 5.0 ± 0.3 (<i>n</i> = 2497) within 7 h after pH bioreporter loading to hyphal surfaces. The pH along hyphal surfaces varied significantly (<i>p</i> < 0.05), with pH at hyphal tips being on average ~0.8 pH units lower than at more mature hyphal parts near the entrance of the microfluidic observation chamber. Our data represent the first dynamic in vitro analysis of surface pH along growing hyphae at the micrometre scale. Such knowledge may improve our understanding of spatial, pH-dependent hyphal processes, such as the degradation of organic matter or mineral weathering.https://www.mdpi.com/2309-608X/8/6/599bioreportermicrofluidicshyphospheremycosphere<i>Coprinopsis cinerea</i>single cell |
| spellingShingle | Bi-Jing Xiong Claire E. Stanley Christian Dusny Dietmar Schlosser Hauke Harms Lukas Y. Wick pH Distribution along Growing Fungal Hyphae at Microscale Journal of Fungi bioreporter microfluidics hyphosphere mycosphere <i>Coprinopsis cinerea</i> single cell |
| title | pH Distribution along Growing Fungal Hyphae at Microscale |
| title_full | pH Distribution along Growing Fungal Hyphae at Microscale |
| title_fullStr | pH Distribution along Growing Fungal Hyphae at Microscale |
| title_full_unstemmed | pH Distribution along Growing Fungal Hyphae at Microscale |
| title_short | pH Distribution along Growing Fungal Hyphae at Microscale |
| title_sort | ph distribution along growing fungal hyphae at microscale |
| topic | bioreporter microfluidics hyphosphere mycosphere <i>Coprinopsis cinerea</i> single cell |
| url | https://www.mdpi.com/2309-608X/8/6/599 |
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