Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus
Abstract Background Porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (PEDV) has led to serious economic losses to the swine industry worldwide. In this study, an oral recombinant Lactobacillus casei vaccine against PEDV infection targeting the intestinal microfold (M) cells and de...
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-018-0861-7 |
| _version_ | 1811268571692531712 |
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| author | Sunting Ma Li Wang Xuewei Huang Xiaona Wang Su Chen Wen Shi Xinyuan Qiao Yanping Jiang Lijie Tang Yigang Xu Yijing Li |
| author_facet | Sunting Ma Li Wang Xuewei Huang Xiaona Wang Su Chen Wen Shi Xinyuan Qiao Yanping Jiang Lijie Tang Yigang Xu Yijing Li |
| author_sort | Sunting Ma |
| collection | DOAJ |
| description | Abstract Background Porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (PEDV) has led to serious economic losses to the swine industry worldwide. In this study, an oral recombinant Lactobacillus casei vaccine against PEDV infection targeting the intestinal microfold (M) cells and dendritic cells (DCs) for delivering the core neutralizing epitope (COE) of PEDV spike protein was developed with M cell-targeting peptide (Col) and dendritic cell-targeting peptide (DCpep). The immunogenicity of the orally administered recombinant strains was evaluated. Results After immunization, significantly higher levels of anti-PEDV specific IgG antibodies with PEDV neutralizing activity in the sera and mucosal sIgA antibodies in the tractus genitalis, intestinal mucus, and stools were detected in mice orally administered with the recombinant strain pPG-COE-Col-DCpep/L393, which expressed DCpep and Col targeting ligands fused with the PEDV COE antigen, compared to mice orally immunized with the recombinant strain pPG-COE/L393 without the DCpep and Col targeting ligands. Moreover, in response to restimulation with the PEDV COE antigen in vitro, a significant difference in splenocyte proliferation response and Th2-associated cytokine IL-4 level was observed in the group of mice orally immunized with pPG-COE-Col-DCpep/L393 (p < 0.05) compared to the groups of mice that received pPG-COE-Col/L393 and pPG-COE-DCpep/L393, respectively. Conclusions The intestinal M cells- and DCs-targeting oral delivery of genetically engineered Lactobacillus expressing the COE antigen of PEDV can efficiently induce anti-PEDV mucosal, humoral, and cellular immune responses via oral administration, suggesting a promising vaccine strategy against PEDV infection. |
| first_indexed | 2024-04-12T21:24:44Z |
| format | Article |
| id | doaj.art-841cdf8974c24dc680395efde1282188 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-12T21:24:44Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-841cdf8974c24dc680395efde12821882022-12-22T03:16:12ZengBMCMicrobial Cell Factories1475-28592018-02-0117111210.1186/s12934-018-0861-7Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virusSunting Ma0Li Wang1Xuewei Huang2Xiaona Wang3Su Chen4Wen Shi5Xinyuan Qiao6Yanping Jiang7Lijie Tang8Yigang Xu9Yijing Li10College of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityAbstract Background Porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (PEDV) has led to serious economic losses to the swine industry worldwide. In this study, an oral recombinant Lactobacillus casei vaccine against PEDV infection targeting the intestinal microfold (M) cells and dendritic cells (DCs) for delivering the core neutralizing epitope (COE) of PEDV spike protein was developed with M cell-targeting peptide (Col) and dendritic cell-targeting peptide (DCpep). The immunogenicity of the orally administered recombinant strains was evaluated. Results After immunization, significantly higher levels of anti-PEDV specific IgG antibodies with PEDV neutralizing activity in the sera and mucosal sIgA antibodies in the tractus genitalis, intestinal mucus, and stools were detected in mice orally administered with the recombinant strain pPG-COE-Col-DCpep/L393, which expressed DCpep and Col targeting ligands fused with the PEDV COE antigen, compared to mice orally immunized with the recombinant strain pPG-COE/L393 without the DCpep and Col targeting ligands. Moreover, in response to restimulation with the PEDV COE antigen in vitro, a significant difference in splenocyte proliferation response and Th2-associated cytokine IL-4 level was observed in the group of mice orally immunized with pPG-COE-Col-DCpep/L393 (p < 0.05) compared to the groups of mice that received pPG-COE-Col/L393 and pPG-COE-DCpep/L393, respectively. Conclusions The intestinal M cells- and DCs-targeting oral delivery of genetically engineered Lactobacillus expressing the COE antigen of PEDV can efficiently induce anti-PEDV mucosal, humoral, and cellular immune responses via oral administration, suggesting a promising vaccine strategy against PEDV infection.http://link.springer.com/article/10.1186/s12934-018-0861-7LactobacillusPorcine epidemic diarrhea virusDendritic cell-targeting peptideMicrofold cell-targeting peptideOral immunization |
| spellingShingle | Sunting Ma Li Wang Xuewei Huang Xiaona Wang Su Chen Wen Shi Xinyuan Qiao Yanping Jiang Lijie Tang Yigang Xu Yijing Li Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus Microbial Cell Factories Lactobacillus Porcine epidemic diarrhea virus Dendritic cell-targeting peptide Microfold cell-targeting peptide Oral immunization |
| title | Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus |
| title_full | Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus |
| title_fullStr | Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus |
| title_full_unstemmed | Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus |
| title_short | Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus |
| title_sort | oral recombinant lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus |
| topic | Lactobacillus Porcine epidemic diarrhea virus Dendritic cell-targeting peptide Microfold cell-targeting peptide Oral immunization |
| url | http://link.springer.com/article/10.1186/s12934-018-0861-7 |
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