Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice
Fertility preservation through ovarian stimulation, aiming at cryopreserving mature oocytes or embryos, is sometimes unsuccessful. This clinical situation deserves novel approaches to overcome infertility following cancer treatment in patients facing highly gonadotoxic treatment. In this controlled...
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MDPI AG
2021-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/13/2757 |
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| author | Xia Hao Amandine Anastácio Kenny A. Rodriguez-Wallberg |
| author_facet | Xia Hao Amandine Anastácio Kenny A. Rodriguez-Wallberg |
| author_sort | Xia Hao |
| collection | DOAJ |
| description | Fertility preservation through ovarian stimulation, aiming at cryopreserving mature oocytes or embryos, is sometimes unsuccessful. This clinical situation deserves novel approaches to overcome infertility following cancer treatment in patients facing highly gonadotoxic treatment. In this controlled experimental study, we investigated the feasibility of in-vitro culturing secondary follicles isolated from superovulated ovaries of mice recently treated with gonadotropins. The follicle yields of superovulated ovaries were 45.9% less than in unstimulated controls. Follicles from superovulated ovaries showed faster growth pace during the initial 7 days of culture and secreted more 17β-estradiol by the end of culture <i>vs</i> controls. Parameters reflecting the outcome of follicular development and oocyte maturation competence in vitro were similar between superovulated and control groups, with a similar follicle size at the end of culture and around 70% survival. Nearly half of cultured follicles met the criteria for in-vitro maturation in both groups and approximately 60% of those achieved a mature MII oocyte, similarly in both groups. Over 60% of obtained MII oocytes displayed normal-looking spindle and chromosome configurations, without significant differences between the groups. Using a validated follicle culture system, we demonstrated the feasibility of secondary follicle isolation, in-vitro culture and oocyte maturation with normal spindle and chromosome configurations obtained from superovulated mice ovaries. |
| first_indexed | 2024-03-10T10:07:36Z |
| format | Article |
| id | doaj.art-841f6c03cf5b4d85ae8c1e4a3cbd59b4 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T10:07:36Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-841f6c03cf5b4d85ae8c1e4a3cbd59b42023-11-22T01:24:42ZengMDPI AGJournal of Clinical Medicine2077-03832021-06-011013275710.3390/jcm10132757Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using MiceXia Hao0Amandine Anastácio1Kenny A. Rodriguez-Wallberg2Department of Oncology and Pathology, Karolinska Institutet, Solna, SE-171 76 Stockholm, SwedenDepartment of Oncology and Pathology, Karolinska Institutet, Solna, SE-171 76 Stockholm, SwedenDepartment of Oncology and Pathology, Karolinska Institutet, Solna, SE-171 76 Stockholm, SwedenFertility preservation through ovarian stimulation, aiming at cryopreserving mature oocytes or embryos, is sometimes unsuccessful. This clinical situation deserves novel approaches to overcome infertility following cancer treatment in patients facing highly gonadotoxic treatment. In this controlled experimental study, we investigated the feasibility of in-vitro culturing secondary follicles isolated from superovulated ovaries of mice recently treated with gonadotropins. The follicle yields of superovulated ovaries were 45.9% less than in unstimulated controls. Follicles from superovulated ovaries showed faster growth pace during the initial 7 days of culture and secreted more 17β-estradiol by the end of culture <i>vs</i> controls. Parameters reflecting the outcome of follicular development and oocyte maturation competence in vitro were similar between superovulated and control groups, with a similar follicle size at the end of culture and around 70% survival. Nearly half of cultured follicles met the criteria for in-vitro maturation in both groups and approximately 60% of those achieved a mature MII oocyte, similarly in both groups. Over 60% of obtained MII oocytes displayed normal-looking spindle and chromosome configurations, without significant differences between the groups. Using a validated follicle culture system, we demonstrated the feasibility of secondary follicle isolation, in-vitro culture and oocyte maturation with normal spindle and chromosome configurations obtained from superovulated mice ovaries.https://www.mdpi.com/2077-0383/10/13/2757female fertility preservationsuperovulationfollicle isolationsecondary follicle culturein-vitro maturationovarian tissue |
| spellingShingle | Xia Hao Amandine Anastácio Kenny A. Rodriguez-Wallberg Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice Journal of Clinical Medicine female fertility preservation superovulation follicle isolation secondary follicle culture in-vitro maturation ovarian tissue |
| title | Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice |
| title_full | Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice |
| title_fullStr | Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice |
| title_full_unstemmed | Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice |
| title_short | Feasibility of Secondary Follicle Isolation, Culture and Achievement of In-Vitro Oocyte Maturation from Superovulated Ovaries: An Experimental Proof-of-Concept Study Using Mice |
| title_sort | feasibility of secondary follicle isolation culture and achievement of in vitro oocyte maturation from superovulated ovaries an experimental proof of concept study using mice |
| topic | female fertility preservation superovulation follicle isolation secondary follicle culture in-vitro maturation ovarian tissue |
| url | https://www.mdpi.com/2077-0383/10/13/2757 |
| work_keys_str_mv | AT xiahao feasibilityofsecondaryfollicleisolationcultureandachievementofinvitrooocytematurationfromsuperovulatedovariesanexperimentalproofofconceptstudyusingmice AT amandineanastacio feasibilityofsecondaryfollicleisolationcultureandachievementofinvitrooocytematurationfromsuperovulatedovariesanexperimentalproofofconceptstudyusingmice AT kennyarodriguezwallberg feasibilityofsecondaryfollicleisolationcultureandachievementofinvitrooocytematurationfromsuperovulatedovariesanexperimentalproofofconceptstudyusingmice |