Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation
Abstract Objective In the present study, we investigated different simple and cost effective methods to evaluate and validate cell free DNA (cfDNA) isolation. The ability of the QIAamp DNA Blood Mini Kit method to extract cfDNA was assessed by several approaches, including purification of endogenous...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3866-8 |
| _version_ | 1818142676760920064 |
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| author | Afsaneh Mojtabanezhad Shariatpanahi Parisa Rokni Elaheh Shahabi Fatemeh Varshoee Tabrizi Mohammad Amin Kerachian |
| author_facet | Afsaneh Mojtabanezhad Shariatpanahi Parisa Rokni Elaheh Shahabi Fatemeh Varshoee Tabrizi Mohammad Amin Kerachian |
| author_sort | Afsaneh Mojtabanezhad Shariatpanahi |
| collection | DOAJ |
| description | Abstract Objective In the present study, we investigated different simple and cost effective methods to evaluate and validate cell free DNA (cfDNA) isolation. The ability of the QIAamp DNA Blood Mini Kit method to extract cfDNA was assessed by several approaches, including purification of endogenous cfDNA and exogenous spike-in control material, prior to plasma extraction, and followed by quantitative-PCR. Results Using QIAamp DNA Blood Mini kit, nearly 27% (380 bp) to 35% (173 bp) cfDNA was recovered with a higher recovery of smaller size cfDNA (173 bp) in comparison to larger ones (380 bp). These simple laboratory methods can be used to assess the efficiency of any cfDNA isolation method. |
| first_indexed | 2024-12-11T11:19:34Z |
| format | Article |
| id | doaj.art-84209c2fa4a44e8591d0737c6961c895 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-11T11:19:34Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-84209c2fa4a44e8591d0737c6961c8952022-12-22T01:09:13ZengBMCBMC Research Notes1756-05002018-10-011111610.1186/s13104-018-3866-8Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolationAfsaneh Mojtabanezhad Shariatpanahi0Parisa Rokni1Elaheh Shahabi2Fatemeh Varshoee Tabrizi3Mohammad Amin Kerachian4Cancer Genetics Research Unit, Reza Radiotherapy and Oncology CenterCancer Genetics Research Unit, Reza Radiotherapy and Oncology CenterCancer Genetics Research Unit, Reza Radiotherapy and Oncology CenterCancer Genetics Research Unit, Reza Radiotherapy and Oncology CenterCancer Genetics Research Unit, Reza Radiotherapy and Oncology CenterAbstract Objective In the present study, we investigated different simple and cost effective methods to evaluate and validate cell free DNA (cfDNA) isolation. The ability of the QIAamp DNA Blood Mini Kit method to extract cfDNA was assessed by several approaches, including purification of endogenous cfDNA and exogenous spike-in control material, prior to plasma extraction, and followed by quantitative-PCR. Results Using QIAamp DNA Blood Mini kit, nearly 27% (380 bp) to 35% (173 bp) cfDNA was recovered with a higher recovery of smaller size cfDNA (173 bp) in comparison to larger ones (380 bp). These simple laboratory methods can be used to assess the efficiency of any cfDNA isolation method.http://link.springer.com/article/10.1186/s13104-018-3866-8PlasmaLiquid biopsyCell-free DNA |
| spellingShingle | Afsaneh Mojtabanezhad Shariatpanahi Parisa Rokni Elaheh Shahabi Fatemeh Varshoee Tabrizi Mohammad Amin Kerachian Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation BMC Research Notes Plasma Liquid biopsy Cell-free DNA |
| title | Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation |
| title_full | Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation |
| title_fullStr | Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation |
| title_full_unstemmed | Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation |
| title_short | Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation |
| title_sort | simple and cost effective laboratory methods to evaluate and validate cell free dna isolation |
| topic | Plasma Liquid biopsy Cell-free DNA |
| url | http://link.springer.com/article/10.1186/s13104-018-3866-8 |
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