Molecular Variation of the Phytopathogenic Fungus Sporisorium reilianum in Valle del Mezquital, Hidalgo
Head smut in maize (Zea mays) is a systemic disease caused by the phytopathogenic fungus Sporisorium reilianum. One of the most severely affected regions in Mexico is Valle del Mezquital in the state of Hidalgo, a high production zone with irrigated agriculture that produces over 50% of maize suppli...
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fevo.2020.00036/full |
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| author | Hasdra Elena Sánchez Maya Yuridia Mercado-Flores Alejandro Téllez-Jurado Juan Pablo Pérez-Camarillo Omar Mejía Miguel Angel Anducho-Reyes |
| author_facet | Hasdra Elena Sánchez Maya Yuridia Mercado-Flores Alejandro Téllez-Jurado Juan Pablo Pérez-Camarillo Omar Mejía Miguel Angel Anducho-Reyes |
| author_sort | Hasdra Elena Sánchez Maya |
| collection | DOAJ |
| description | Head smut in maize (Zea mays) is a systemic disease caused by the phytopathogenic fungus Sporisorium reilianum. One of the most severely affected regions in Mexico is Valle del Mezquital in the state of Hidalgo, a high production zone with irrigated agriculture that produces over 50% of maize supplies in the state. This study was designed to analyze the genetic diversity of this phytopathogen isolated from different zones of Valle del Mezquital using the following molecular markers: ITS, aspartyl protease, β-tubulin, and the mitochondrial endonuclease LAGLIDADG. Our objective was to estimate the level of genetic variability in a regional space and understand the evolutionary processes to which non-model organisms are subjected. A total of 53 strains of S. reilianum were isolated from the sampling sites and then purified. A Bayesian Analysis of Population Structure (BAPS) analysis of the genetic structuring allowed us to recover three genetic groupings (K = 3), while molecular variance analysis (AMOVA) showed a very low fixation index (FST = 0.0146), which was not statistically significant and indicated little between-group genetic differentiation. Also, the value determined for the coefficient of genetic differentiation (Gst) showed little variation among the genetic groups, while the gene flow number (Nm) was 8.25. The Tajima’s D estimator and haplotype network recently indicated a rapid population expansion. Together, these results suggest that reproduction and migration are key factors in the evolution and virulence of this fungus. |
| first_indexed | 2024-12-22T03:49:47Z |
| format | Article |
| id | doaj.art-842370e918f944f88fbaf95c90c280a7 |
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| issn | 2296-701X |
| language | English |
| last_indexed | 2024-12-22T03:49:47Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-842370e918f944f88fbaf95c90c280a72022-12-21T18:40:01ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2020-02-01810.3389/fevo.2020.00036490344Molecular Variation of the Phytopathogenic Fungus Sporisorium reilianum in Valle del Mezquital, HidalgoHasdra Elena Sánchez Maya0Yuridia Mercado-Flores1Alejandro Téllez-Jurado2Juan Pablo Pérez-Camarillo3Omar Mejía4Miguel Angel Anducho-Reyes5Laboratorio de Agrobiotecnología, Universidad Politécnica de Pachuca, Zempoala, MexicoLaboratorio de Agrobiotecnología, Universidad Politécnica de Pachuca, Zempoala, MexicoLaboratorio de Agrobiotecnología, Universidad Politécnica de Pachuca, Zempoala, MexicoCentro de Investigación y Desarrollo en Agrobiotecnología Alimentaria, Pachuca Ciudad del Conocimiento y la Cultura, San Agustín Tlaxiaca, MexicoDepartamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Miguel Hidalgo, MexicoLaboratorio de Agrobiotecnología, Universidad Politécnica de Pachuca, Zempoala, MexicoHead smut in maize (Zea mays) is a systemic disease caused by the phytopathogenic fungus Sporisorium reilianum. One of the most severely affected regions in Mexico is Valle del Mezquital in the state of Hidalgo, a high production zone with irrigated agriculture that produces over 50% of maize supplies in the state. This study was designed to analyze the genetic diversity of this phytopathogen isolated from different zones of Valle del Mezquital using the following molecular markers: ITS, aspartyl protease, β-tubulin, and the mitochondrial endonuclease LAGLIDADG. Our objective was to estimate the level of genetic variability in a regional space and understand the evolutionary processes to which non-model organisms are subjected. A total of 53 strains of S. reilianum were isolated from the sampling sites and then purified. A Bayesian Analysis of Population Structure (BAPS) analysis of the genetic structuring allowed us to recover three genetic groupings (K = 3), while molecular variance analysis (AMOVA) showed a very low fixation index (FST = 0.0146), which was not statistically significant and indicated little between-group genetic differentiation. Also, the value determined for the coefficient of genetic differentiation (Gst) showed little variation among the genetic groups, while the gene flow number (Nm) was 8.25. The Tajima’s D estimator and haplotype network recently indicated a rapid population expansion. Together, these results suggest that reproduction and migration are key factors in the evolution and virulence of this fungus.https://www.frontiersin.org/article/10.3389/fevo.2020.00036/fullhead smutLAGLIDADGBAPSmaizephytopathogenic |
| spellingShingle | Hasdra Elena Sánchez Maya Yuridia Mercado-Flores Alejandro Téllez-Jurado Juan Pablo Pérez-Camarillo Omar Mejía Miguel Angel Anducho-Reyes Molecular Variation of the Phytopathogenic Fungus Sporisorium reilianum in Valle del Mezquital, Hidalgo Frontiers in Ecology and Evolution head smut LAGLIDADG BAPS maize phytopathogenic |
| title | Molecular Variation of the Phytopathogenic Fungus Sporisorium reilianum in Valle del Mezquital, Hidalgo |
| title_full | Molecular Variation of the Phytopathogenic Fungus Sporisorium reilianum in Valle del Mezquital, Hidalgo |
| title_fullStr | Molecular Variation of the Phytopathogenic Fungus Sporisorium reilianum in Valle del Mezquital, Hidalgo |
| title_full_unstemmed | Molecular Variation of the Phytopathogenic Fungus Sporisorium reilianum in Valle del Mezquital, Hidalgo |
| title_short | Molecular Variation of the Phytopathogenic Fungus Sporisorium reilianum in Valle del Mezquital, Hidalgo |
| title_sort | molecular variation of the phytopathogenic fungus sporisorium reilianum in valle del mezquital hidalgo |
| topic | head smut LAGLIDADG BAPS maize phytopathogenic |
| url | https://www.frontiersin.org/article/10.3389/fevo.2020.00036/full |
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