Effects of Exogenous Caffeic Acid, L-Phenylalanine and NaCl Treatments on Main Active Components Content and <i>In Vitro</i> Digestion of Germinated Tartary Buckwheat
Germination is an effective method for improving the nutritional value of Tartary buckwheat (TB). The effects of exogenous additive treatments (caffeic acid (CA), L-phenylalanine (L-Phe), NaCl) on germination, main active component contents and antioxidant activities before and after <i>in vit...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/22/3682 |
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| author | Wenping Peng Nan Wang Shunmin Wang Junzhen Wang Yulu Dong |
| author_facet | Wenping Peng Nan Wang Shunmin Wang Junzhen Wang Yulu Dong |
| author_sort | Wenping Peng |
| collection | DOAJ |
| description | Germination is an effective method for improving the nutritional value of Tartary buckwheat (TB). The effects of exogenous additive treatments (caffeic acid (CA), L-phenylalanine (L-Phe), NaCl) on germination, main active component contents and antioxidant activities before and after <i>in vitro</i> digestion of germinated TB were investigated. Compared with the natural growth group, the T<sub>4</sub> group (CA 17 mg/L, L-Phe 2.7 mmol/L, NaCl 2.7 mmol/L) treatment increased the germination rate (67.50%), sprout length, reducing sugar (53.05%), total flavonoid (18.36%) and total phenolic (20.96%) content, and antioxidant capacity of TB. In addition, exogenous additives treatment induced the consumption of a lot of nutrients during seed germination, resulting in a decrease in the content of soluble protein and soluble sugar. The stress degree of natural germination on seeds was higher than that of low concentrations of exogenous additives, resulting in an increase in malondialdehyde content. <i>In vitro</i> digestion leads to a decrease in phenolics content and antioxidant capacity, which can be alleviated by exogenous treatment. The results showed that treatment with exogenous additives was a good method to increase the nutritional value of germinated TB, which provided a theoretical basis for screening suitable growth conditions for flavonoid enrichment. |
| first_indexed | 2024-03-09T18:19:44Z |
| format | Article |
| id | doaj.art-8424f98e586b4fcb8132ab82b728e011 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T18:19:44Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-8424f98e586b4fcb8132ab82b728e0112023-11-24T08:22:37ZengMDPI AGFoods2304-81582022-11-011122368210.3390/foods11223682Effects of Exogenous Caffeic Acid, L-Phenylalanine and NaCl Treatments on Main Active Components Content and <i>In Vitro</i> Digestion of Germinated Tartary BuckwheatWenping Peng0Nan Wang1Shunmin Wang2Junzhen Wang3Yulu Dong4College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaCollege of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaCollege of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaAcademy of Agricultural Science Liang Shan, Liangshan 615000, ChinaCollege of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, ChinaGermination is an effective method for improving the nutritional value of Tartary buckwheat (TB). The effects of exogenous additive treatments (caffeic acid (CA), L-phenylalanine (L-Phe), NaCl) on germination, main active component contents and antioxidant activities before and after <i>in vitro</i> digestion of germinated TB were investigated. Compared with the natural growth group, the T<sub>4</sub> group (CA 17 mg/L, L-Phe 2.7 mmol/L, NaCl 2.7 mmol/L) treatment increased the germination rate (67.50%), sprout length, reducing sugar (53.05%), total flavonoid (18.36%) and total phenolic (20.96%) content, and antioxidant capacity of TB. In addition, exogenous additives treatment induced the consumption of a lot of nutrients during seed germination, resulting in a decrease in the content of soluble protein and soluble sugar. The stress degree of natural germination on seeds was higher than that of low concentrations of exogenous additives, resulting in an increase in malondialdehyde content. <i>In vitro</i> digestion leads to a decrease in phenolics content and antioxidant capacity, which can be alleviated by exogenous treatment. The results showed that treatment with exogenous additives was a good method to increase the nutritional value of germinated TB, which provided a theoretical basis for screening suitable growth conditions for flavonoid enrichment.https://www.mdpi.com/2304-8158/11/22/3682Tartary buckwheatcaffeic acidL-phenylalanineNaClgermination<i>in vitro</i> digestion |
| spellingShingle | Wenping Peng Nan Wang Shunmin Wang Junzhen Wang Yulu Dong Effects of Exogenous Caffeic Acid, L-Phenylalanine and NaCl Treatments on Main Active Components Content and <i>In Vitro</i> Digestion of Germinated Tartary Buckwheat Foods Tartary buckwheat caffeic acid L-phenylalanine NaCl germination <i>in vitro</i> digestion |
| title | Effects of Exogenous Caffeic Acid, L-Phenylalanine and NaCl Treatments on Main Active Components Content and <i>In Vitro</i> Digestion of Germinated Tartary Buckwheat |
| title_full | Effects of Exogenous Caffeic Acid, L-Phenylalanine and NaCl Treatments on Main Active Components Content and <i>In Vitro</i> Digestion of Germinated Tartary Buckwheat |
| title_fullStr | Effects of Exogenous Caffeic Acid, L-Phenylalanine and NaCl Treatments on Main Active Components Content and <i>In Vitro</i> Digestion of Germinated Tartary Buckwheat |
| title_full_unstemmed | Effects of Exogenous Caffeic Acid, L-Phenylalanine and NaCl Treatments on Main Active Components Content and <i>In Vitro</i> Digestion of Germinated Tartary Buckwheat |
| title_short | Effects of Exogenous Caffeic Acid, L-Phenylalanine and NaCl Treatments on Main Active Components Content and <i>In Vitro</i> Digestion of Germinated Tartary Buckwheat |
| title_sort | effects of exogenous caffeic acid l phenylalanine and nacl treatments on main active components content and i in vitro i digestion of germinated tartary buckwheat |
| topic | Tartary buckwheat caffeic acid L-phenylalanine NaCl germination <i>in vitro</i> digestion |
| url | https://www.mdpi.com/2304-8158/11/22/3682 |
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