Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genus
The aim of the paper is to compare the efficacy of SCAR-PCR assay and conventional diagnostic technique (visual assessment, isolation on PDA medium) in the identification of fungi from the genera Rhizoctonia and Oculimacula from winter triticale, rye, and barley during the shooting stage. The useful...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2012-12-01
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| Series: | Acta Agrobotanica |
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| Online Access: | https://pbsociety.org.pl/journals/index.php/aa/article/view/1455 |
| _version_ | 1819104310888235008 |
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| author | Grzegorz Lemańczyk |
| author_facet | Grzegorz Lemańczyk |
| author_sort | Grzegorz Lemańczyk |
| collection | DOAJ |
| description | The aim of the paper is to compare the efficacy of SCAR-PCR assay and conventional diagnostic technique (visual assessment, isolation on PDA medium) in the identification of fungi from the genera Rhizoctonia and Oculimacula from winter triticale, rye, and barley during the shooting stage. The usefulness of molecular diagnosis of fungal pathogens in crop plants has been demonstrated. The application of SCAR- -PCR assay allowed early detection of the following pathogens: O. yallundae, O. acuformis, R. cerealis and R. solani, in plant tissues. This method was particularly effective in detection of R. solani. The research showed the usefulness of PCR markers for early detection of fungal pathogens, even if symptoms were not visible. Using the PCR technique, especially in combination with conventional methods, substantially increases the precision and effectiveness of disease diagnostics. |
| first_indexed | 2024-12-22T02:04:20Z |
| format | Article |
| id | doaj.art-84263e4484b24223a8d102957faaf843 |
| institution | Directory Open Access Journal |
| issn | 2300-357X |
| language | English |
| last_indexed | 2024-12-22T02:04:20Z |
| publishDate | 2012-12-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Agrobotanica |
| spelling | doaj.art-84263e4484b24223a8d102957faaf8432022-12-21T18:42:34ZengPolish Botanical SocietyActa Agrobotanica2300-357X2012-12-0164419720610.5586/aa.2011.0611122Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genusGrzegorz Lemańczyk0University of Technology and Life Sciences in BydgoszczThe aim of the paper is to compare the efficacy of SCAR-PCR assay and conventional diagnostic technique (visual assessment, isolation on PDA medium) in the identification of fungi from the genera Rhizoctonia and Oculimacula from winter triticale, rye, and barley during the shooting stage. The usefulness of molecular diagnosis of fungal pathogens in crop plants has been demonstrated. The application of SCAR- -PCR assay allowed early detection of the following pathogens: O. yallundae, O. acuformis, R. cerealis and R. solani, in plant tissues. This method was particularly effective in detection of R. solani. The research showed the usefulness of PCR markers for early detection of fungal pathogens, even if symptoms were not visible. Using the PCR technique, especially in combination with conventional methods, substantially increases the precision and effectiveness of disease diagnostics.https://pbsociety.org.pl/journals/index.php/aa/article/view/1455PCR assaysharp eyespoteyespotdiagnosticsbarleytriticalerye |
| spellingShingle | Grzegorz Lemańczyk Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genus Acta Agrobotanica PCR assay sharp eyespot eyespot diagnostics barley triticale rye |
| title | Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genus |
| title_full | Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genus |
| title_fullStr | Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genus |
| title_full_unstemmed | Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genus |
| title_short | Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genus |
| title_sort | use of scar pcr in diagnostics of stem base pathogens of the rhizoctonia and oculimacula genus |
| topic | PCR assay sharp eyespot eyespot diagnostics barley triticale rye |
| url | https://pbsociety.org.pl/journals/index.php/aa/article/view/1455 |
| work_keys_str_mv | AT grzegorzlemanczyk useofscarpcrindiagnosticsofstembasepathogensoftherhizoctoniaandoculimaculagenus |