DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohort
The biological mechanisms through which adherence to Mediterranean Diet (MD) protects against colon cancer (CC) are poorly understood. Evidence suggests that chronic inflammation may be implicated in the pathway. Both diet and CC are related to epigenetic regulation. We performed a nested case-contr...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-10-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2019.1629230 |
| _version_ | 1797678898804162560 |
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| author | Francesca Fasanelli Maria Teresa Giraudo Paolo Vineis Valentina Fiano Giovanni Fiorito Chiara Grasso Silvia Polidoro Morena Trevisan Sara Grioni Vittorio Krogh Amalia Mattiello Salvatore Panico Maria Concetta Giurdanella Rosario Tumino Laura De Marco Fulvio Ricceri Carlotta Sacerdote |
| author_facet | Francesca Fasanelli Maria Teresa Giraudo Paolo Vineis Valentina Fiano Giovanni Fiorito Chiara Grasso Silvia Polidoro Morena Trevisan Sara Grioni Vittorio Krogh Amalia Mattiello Salvatore Panico Maria Concetta Giurdanella Rosario Tumino Laura De Marco Fulvio Ricceri Carlotta Sacerdote |
| author_sort | Francesca Fasanelli |
| collection | DOAJ |
| description | The biological mechanisms through which adherence to Mediterranean Diet (MD) protects against colon cancer (CC) are poorly understood. Evidence suggests that chronic inflammation may be implicated in the pathway. Both diet and CC are related to epigenetic regulation. We performed a nested case-control study on 161 pairs from the Italian component of the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort, in which we looked for the methylation signals in DNA extracted from leucocytes associated with both CC and MD in 995 CpGs located in 48 inflammation genes. The DNA methylation signals detected in this analysis were validated in a subgroup of 47 case-control pairs and further replicated (where validated) in 95 new pairs by means of pyrosequencing. Among the CpG sites selected a-priori in inflammation-related genes, seven CpG sites were found to be associated with CC status and with MD, in line with its protective effect. Only two CpG sites (cg17968347-SERPINE1 and cg20674490-RUNX3) were validated using bisulphite pyrosequencing and, after replication, we found that DNA methylation of cg20674490-RUNX3 may be a potential molecular mediator explaining the protective effect of MD on CC onset. The use of a ‘meet-in-the-middle’ approach to identify the overlap between exposure and predictive markers of disease is innovative in studies on the relationship between diet and cancer, in which exposure assessment is difficult and the mechanisms through which the nutrients exert their protective effect is largely unknown. |
| first_indexed | 2024-03-11T23:06:37Z |
| format | Article |
| id | doaj.art-84274fe5c61845269d6f8d223c8fc518 |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:06:37Z |
| publishDate | 2019-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-84274fe5c61845269d6f8d223c8fc5182023-09-21T13:09:22ZengTaylor & Francis GroupEpigenetics1559-22941559-23082019-10-01141097798810.1080/15592294.2019.16292301629230DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohortFrancesca Fasanelli0Maria Teresa Giraudo1Paolo Vineis2Valentina Fiano3Giovanni Fiorito4Chiara Grasso5Silvia Polidoro6Morena Trevisan7Sara Grioni8Vittorio Krogh9Amalia Mattiello10Salvatore Panico11Maria Concetta Giurdanella12Rosario Tumino13Laura De Marco14Fulvio Ricceri15Carlotta Sacerdote16University of TurinUniversity of TurinImperial College LondonUniversity of TurinItalian Institute for Genomic Medicine (IIGM)University of TurinItalian Institute for Genomic Medicine (IIGM)University of TurinFondazione IRCCS Istituto Nazionale dei TumoriFondazione IRCCS Istituto Nazionale dei TumoriFederico II UniversityFederico II UniversityASPASPUniversity of TurinRegional Health Services ASL TO3Città della Salute e della Scienza University-Hospital and Center for Cancer Prevention (CPO)The biological mechanisms through which adherence to Mediterranean Diet (MD) protects against colon cancer (CC) are poorly understood. Evidence suggests that chronic inflammation may be implicated in the pathway. Both diet and CC are related to epigenetic regulation. We performed a nested case-control study on 161 pairs from the Italian component of the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort, in which we looked for the methylation signals in DNA extracted from leucocytes associated with both CC and MD in 995 CpGs located in 48 inflammation genes. The DNA methylation signals detected in this analysis were validated in a subgroup of 47 case-control pairs and further replicated (where validated) in 95 new pairs by means of pyrosequencing. Among the CpG sites selected a-priori in inflammation-related genes, seven CpG sites were found to be associated with CC status and with MD, in line with its protective effect. Only two CpG sites (cg17968347-SERPINE1 and cg20674490-RUNX3) were validated using bisulphite pyrosequencing and, after replication, we found that DNA methylation of cg20674490-RUNX3 may be a potential molecular mediator explaining the protective effect of MD on CC onset. The use of a ‘meet-in-the-middle’ approach to identify the overlap between exposure and predictive markers of disease is innovative in studies on the relationship between diet and cancer, in which exposure assessment is difficult and the mechanisms through which the nutrients exert their protective effect is largely unknown.http://dx.doi.org/10.1080/15592294.2019.1629230colon cancermediterranean dietinflammationdna methylationmeet-in-the-middle |
| spellingShingle | Francesca Fasanelli Maria Teresa Giraudo Paolo Vineis Valentina Fiano Giovanni Fiorito Chiara Grasso Silvia Polidoro Morena Trevisan Sara Grioni Vittorio Krogh Amalia Mattiello Salvatore Panico Maria Concetta Giurdanella Rosario Tumino Laura De Marco Fulvio Ricceri Carlotta Sacerdote DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohort Epigenetics colon cancer mediterranean diet inflammation dna methylation meet-in-the-middle |
| title | DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohort |
| title_full | DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohort |
| title_fullStr | DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohort |
| title_full_unstemmed | DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohort |
| title_short | DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohort |
| title_sort | dna methylation colon cancer and mediterranean diet results from the epic italy cohort |
| topic | colon cancer mediterranean diet inflammation dna methylation meet-in-the-middle |
| url | http://dx.doi.org/10.1080/15592294.2019.1629230 |
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