Microarray Analysis of Response of <it>Salmonella </it>during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells
<p>Abstract</p> <p>Background</p> <p>Human leukocyte antigen (HLA)-B27 is strongly associated with the development of reactive arthritis (ReA) in humans after salmonellosis. Human monocytic U937 cells transfected with HLA-B27 are less able to eliminate intracellular <...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-07-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/11/456 |
| _version_ | 1818758769886101504 |
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| author | Hinton Jay CD He Qiushui Danino Vittoria Ge Shichao Granfors Kaisa |
| author_facet | Hinton Jay CD He Qiushui Danino Vittoria Ge Shichao Granfors Kaisa |
| author_sort | Hinton Jay CD |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Human leukocyte antigen (HLA)-B27 is strongly associated with the development of reactive arthritis (ReA) in humans after salmonellosis. Human monocytic U937 cells transfected with HLA-B27 are less able to eliminate intracellular <it>Salmonella enterica </it>serovar Enteritidis than those transfected with control HLA antigens (e.g. HLA-A2). To investigate further the mechanisms by which HLA-B27-transfected cells allow increased replication of these bacteria, a DNA-based microarray was used for comparative genomic analysis of <it>S</it>. Enteritidis grown in HLA-B27- or HLA-A2-transfected cells. The microarray consisted of 5080 oligonucleotides from different serovars of <it>Salmonella </it>including <it>S</it>. Enteritidis PT4-specific genes. Bacterial RNA was isolated from the infected HLA-B27- or HLA-A2-transfected cells, reverse-transcribed to cDNA, and hybridized with the oligonucleotides on the microarrays. Some microarray results were confirmed by RT-PCR.</p> <p>Results</p> <p>When gene expression was compared between <it>Salmonella </it>grown in HLA-B27 cells and in HLA-A2 cells, 118 of the 4610 <it>S</it>. Enteritidis-related genes differed in expression at 8 h after infection, but no significant difference was detectable at 2 h after infection. These differentially expressed genes are mainly involved in <it>Salmonella </it>virulence, DNA replication, energy conversion and metabolism, and uptake and metabolism of nutrient substances, etc. The difference suggests HLA-B27-dependent modulation of <it>Salmonella </it>gene expression, resulting in increased <it>Salmonella </it>replication in HLA-B27-positive cells. Among the up-regulated genes were those located in <it>Salmonella </it>pathogenicity island (SPI)-2, which play a central role in intracellular survival and replication of <it>Salmonella</it>.</p> <p>Conclusions</p> <p>This is the first report to show the regulation of <it>Salmonella </it>gene expression by HLA-B27 during infection of host cells. This regulation probably leads to increased <it>Salmonella </it>survival and replication in HLA-B27-positive cells. SPI-2 genes seem to contribute significantly to the increased replication.</p> |
| first_indexed | 2024-12-18T06:32:06Z |
| format | Article |
| id | doaj.art-842894084e75457c94b1f8b8285fdf8a |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-18T06:32:06Z |
| publishDate | 2010-07-01 |
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| series | BMC Genomics |
| spelling | doaj.art-842894084e75457c94b1f8b8285fdf8a2022-12-21T21:17:53ZengBMCBMC Genomics1471-21642010-07-0111145610.1186/1471-2164-11-456Microarray Analysis of Response of <it>Salmonella </it>during Infection of HLA-B27- Transfected Human Macrophage-Like U937 CellsHinton Jay CDHe QiushuiDanino VittoriaGe ShichaoGranfors Kaisa<p>Abstract</p> <p>Background</p> <p>Human leukocyte antigen (HLA)-B27 is strongly associated with the development of reactive arthritis (ReA) in humans after salmonellosis. Human monocytic U937 cells transfected with HLA-B27 are less able to eliminate intracellular <it>Salmonella enterica </it>serovar Enteritidis than those transfected with control HLA antigens (e.g. HLA-A2). To investigate further the mechanisms by which HLA-B27-transfected cells allow increased replication of these bacteria, a DNA-based microarray was used for comparative genomic analysis of <it>S</it>. Enteritidis grown in HLA-B27- or HLA-A2-transfected cells. The microarray consisted of 5080 oligonucleotides from different serovars of <it>Salmonella </it>including <it>S</it>. Enteritidis PT4-specific genes. Bacterial RNA was isolated from the infected HLA-B27- or HLA-A2-transfected cells, reverse-transcribed to cDNA, and hybridized with the oligonucleotides on the microarrays. Some microarray results were confirmed by RT-PCR.</p> <p>Results</p> <p>When gene expression was compared between <it>Salmonella </it>grown in HLA-B27 cells and in HLA-A2 cells, 118 of the 4610 <it>S</it>. Enteritidis-related genes differed in expression at 8 h after infection, but no significant difference was detectable at 2 h after infection. These differentially expressed genes are mainly involved in <it>Salmonella </it>virulence, DNA replication, energy conversion and metabolism, and uptake and metabolism of nutrient substances, etc. The difference suggests HLA-B27-dependent modulation of <it>Salmonella </it>gene expression, resulting in increased <it>Salmonella </it>replication in HLA-B27-positive cells. Among the up-regulated genes were those located in <it>Salmonella </it>pathogenicity island (SPI)-2, which play a central role in intracellular survival and replication of <it>Salmonella</it>.</p> <p>Conclusions</p> <p>This is the first report to show the regulation of <it>Salmonella </it>gene expression by HLA-B27 during infection of host cells. This regulation probably leads to increased <it>Salmonella </it>survival and replication in HLA-B27-positive cells. SPI-2 genes seem to contribute significantly to the increased replication.</p>http://www.biomedcentral.com/1471-2164/11/456 |
| spellingShingle | Hinton Jay CD He Qiushui Danino Vittoria Ge Shichao Granfors Kaisa Microarray Analysis of Response of <it>Salmonella </it>during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells BMC Genomics |
| title | Microarray Analysis of Response of <it>Salmonella </it>during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells |
| title_full | Microarray Analysis of Response of <it>Salmonella </it>during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells |
| title_fullStr | Microarray Analysis of Response of <it>Salmonella </it>during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells |
| title_full_unstemmed | Microarray Analysis of Response of <it>Salmonella </it>during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells |
| title_short | Microarray Analysis of Response of <it>Salmonella </it>during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells |
| title_sort | microarray analysis of response of it salmonella it during infection of hla b27 transfected human macrophage like u937 cells |
| url | http://www.biomedcentral.com/1471-2164/11/456 |
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