Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological Assay
The aim of this study was to evaluate the use of a capture enzyme-linked immunosorbent assay (ELISA) for the detection of LASV-reactive IgG antibodies in <i>Mastomys</i> rodents. The assay was used for laboratory-bred <i>Mastomys</i> rodents, as well as for animals caught in...
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MDPI AG
2022-05-01
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Online Access: | https://www.mdpi.com/1999-4915/14/5/993 |
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author | Hugo Soubrier Umaru Bangura Chris Hoffmann Ayodeji Olayemi Adetunji Samuel Adesina Stephan Günther Lisa Oestereich Elisabeth Fichet-Calvet |
author_facet | Hugo Soubrier Umaru Bangura Chris Hoffmann Ayodeji Olayemi Adetunji Samuel Adesina Stephan Günther Lisa Oestereich Elisabeth Fichet-Calvet |
author_sort | Hugo Soubrier |
collection | DOAJ |
description | The aim of this study was to evaluate the use of a capture enzyme-linked immunosorbent assay (ELISA) for the detection of LASV-reactive IgG antibodies in <i>Mastomys</i> rodents. The assay was used for laboratory-bred <i>Mastomys</i> rodents, as well as for animals caught in the wild in various regions of West Africa. The ELISA reached an accuracy of 97.1% in samples of known exposure, and a comparison to an immunofluorescence assay (IFA) revealed a very strong agreement between the ELISA and IFA results (Cohen’s kappa of 0.81). The agreement is valid in Nigeria, and in Guinea and Sierra Leone where the lineages II and IV are circulating, respectively. Altogether, these results indicate that this capture ELISA is suitable for LASV IgG serostatus determination in <i>Mastomys</i> rodents as an alternative to IFA. This assay will be a strong, accurate, and semi-quantitative alternative for rodent seroprevalence studies that does not depend on biosafety level 4 infrastructures, providing great benefits for ecology and epidemiology studies of Lassa fever, a disease listed on the Research and Development Blueprint of the WHO. |
first_indexed | 2024-03-10T01:36:31Z |
format | Article |
id | doaj.art-842a196a88f945618787163adfe8a842 |
institution | Directory Open Access Journal |
issn | 1999-4915 |
language | English |
last_indexed | 2024-03-10T01:36:31Z |
publishDate | 2022-05-01 |
publisher | MDPI AG |
record_format | Article |
series | Viruses |
spelling | doaj.art-842a196a88f945618787163adfe8a8422023-11-23T13:31:28ZengMDPI AGViruses1999-49152022-05-0114599310.3390/v14050993Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological AssayHugo Soubrier0Umaru Bangura1Chris Hoffmann2Ayodeji Olayemi3Adetunji Samuel Adesina4Stephan Günther5Lisa Oestereich6Elisabeth Fichet-Calvet7Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, GermanyBernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, GermanyBernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, GermanyNatural History Museum, Obafemi Awolowo University, Ile Ife HO220005, NigeriaDepartment of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile Ife HO220005, NigeriaBernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, GermanyBernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, GermanyBernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, GermanyThe aim of this study was to evaluate the use of a capture enzyme-linked immunosorbent assay (ELISA) for the detection of LASV-reactive IgG antibodies in <i>Mastomys</i> rodents. The assay was used for laboratory-bred <i>Mastomys</i> rodents, as well as for animals caught in the wild in various regions of West Africa. The ELISA reached an accuracy of 97.1% in samples of known exposure, and a comparison to an immunofluorescence assay (IFA) revealed a very strong agreement between the ELISA and IFA results (Cohen’s kappa of 0.81). The agreement is valid in Nigeria, and in Guinea and Sierra Leone where the lineages II and IV are circulating, respectively. Altogether, these results indicate that this capture ELISA is suitable for LASV IgG serostatus determination in <i>Mastomys</i> rodents as an alternative to IFA. This assay will be a strong, accurate, and semi-quantitative alternative for rodent seroprevalence studies that does not depend on biosafety level 4 infrastructures, providing great benefits for ecology and epidemiology studies of Lassa fever, a disease listed on the Research and Development Blueprint of the WHO.https://www.mdpi.com/1999-4915/14/5/993ELISAIFA<i>Mastomys</i>natural hostIgGLassa virus |
spellingShingle | Hugo Soubrier Umaru Bangura Chris Hoffmann Ayodeji Olayemi Adetunji Samuel Adesina Stephan Günther Lisa Oestereich Elisabeth Fichet-Calvet Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological Assay Viruses ELISA IFA <i>Mastomys</i> natural host IgG Lassa virus |
title | Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological Assay |
title_full | Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological Assay |
title_fullStr | Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological Assay |
title_full_unstemmed | Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological Assay |
title_short | Detection of Lassa Virus-Reactive IgG Antibodies in Wild Rodents: Validation of a Capture Enzyme-Linked Immunological Assay |
title_sort | detection of lassa virus reactive igg antibodies in wild rodents validation of a capture enzyme linked immunological assay |
topic | ELISA IFA <i>Mastomys</i> natural host IgG Lassa virus |
url | https://www.mdpi.com/1999-4915/14/5/993 |
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