EXPRESSION OF PROTEIN GENES PARTICIPATING IN FIBROPLASTIC PROCESSES IN MICE LUNG DURING THE DEVELOPMENT OF TUBERCULOUS INFLAMMATION
The study analyzes the expression of protein genes involved in intracellular signaling pathways associated with a profibrotic response, activation of epithelial-mesenchymal and endothelial-mesenchymal transitions, in modeling tuberculous granulomatosis and pharmaceutical effects. Material and method...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Siberian Branch Publishing House
2019-09-01
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| Series: | Сибирский научный медицинский журнал |
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| Online Access: | https://sibmed.elpub.ru/jour/article/view/204 |
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| author | P. M. Kozhin A. V. Chechushkov N. S. Zaytseva M. V. Khrapova L. A. Cherdantseva E. B. Menshchikova A. V. Troitsky V. A. Shkurupy |
| author_facet | P. M. Kozhin A. V. Chechushkov N. S. Zaytseva M. V. Khrapova L. A. Cherdantseva E. B. Menshchikova A. V. Troitsky V. A. Shkurupy |
| author_sort | P. M. Kozhin |
| collection | DOAJ |
| description | The study analyzes the expression of protein genes involved in intracellular signaling pathways associated with a profibrotic response, activation of epithelial-mesenchymal and endothelial-mesenchymal transitions, in modeling tuberculous granulomatosis and pharmaceutical effects. Material and methods. The study was performed on male BALB/c mice aged two months and weighing 18–22 g. Generalized tuberculous granulomatosis was simulated by a single intravenous (retro-orbital) injection of 0.5 mg BCG vaccine in 0.2 ml of isotonic aqueous NaCl solution (SS), after 4 months a part of mice started to receive treatment drugs, after 2 months the animals were sacrificed by decapitation (6 months after BCG vaccine administration) and their lung tissues were collected. Mice were divided into 6 groups, 5 males in each: intact, which were intravenously into the retro-orbital sinus injected with 0.2 ml SS (C); infected with BCG and receiving SS intraperitoneal injections (BS); infected with BCG and received intraperitoneal injections of isonicotinic acid hydrazide solution (INAH) (BI); infected with BCG and received intraperitoneal injections of dextrazide solution (conjugate of 40 kDa oxidized dextran and INAH) (BD); infected with BCG and received intraperitoneal or inhalation injections of solution of molecular-nanosomal pharmaceutical compositions of oxidized dextran (MNPC) (BMP and BMH, respectively). Finally, the expression of mRNA of matrix metalloproteinase 9, type III procollagen, TGF-β, and transcription factors ZEB1 and Snai1 was determined by real-time PCR in mice lung tissue. Results. The modeling of generalized tuberculous granulomatosis was found to be accompanied by the induction of epithelial-mesenchymal transition and the activation of profibrotic processes, which after 6 months was manifested in increase of the type III collagen α1-chain and TGF-β mRNA expression. Administration of traditional (INAH) and original (dextrazide, MNPC) preparations with anti-tuberculosis activity within two months has inhibitory activity of varying severity in relation to various markers of these processes. |
| first_indexed | 2024-03-08T14:34:07Z |
| format | Article |
| id | doaj.art-842a758e71d946fd996780b105c1304d |
| institution | Directory Open Access Journal |
| issn | 2410-2512 2410-2520 |
| language | Russian |
| last_indexed | 2024-04-25T01:51:04Z |
| publishDate | 2019-09-01 |
| publisher | Russian Academy of Sciences, Siberian Branch Publishing House |
| record_format | Article |
| series | Сибирский научный медицинский журнал |
| spelling | doaj.art-842a758e71d946fd996780b105c1304d2024-03-07T18:49:58ZrusRussian Academy of Sciences, Siberian Branch Publishing HouseСибирский научный медицинский журнал2410-25122410-25202019-09-01394222910.15372/SSMJ20190403213EXPRESSION OF PROTEIN GENES PARTICIPATING IN FIBROPLASTIC PROCESSES IN MICE LUNG DURING THE DEVELOPMENT OF TUBERCULOUS INFLAMMATIONP. M. Kozhin0A. V. Chechushkov1N. S. Zaytseva2M. V. Khrapova3L. A. Cherdantseva4E. B. Menshchikova5A. V. Troitsky6V. A. Shkurupy7Research Institute for Experimental and Clinical Medicine of Federal Research Center for Basic and Translational MedicineResearch Institute for Experimental and Clinical Medicine of Federal Research Center for Basic and Translational MedicineResearch Institute for Experimental and Clinical Medicine of Federal Research Center for Basic and Translational MedicineResearch Institute for Experimental and Clinical Medicine of Federal Research Center for Basic and Translational MedicineResearch Institute for Experimental and Clinical Medicine of Federal Research Center for Basic and Translational Medicine, Novosibirsk Research Institute of Traumatology and Orthopaedics n.a. Ya.L. TsivyanResearch Institute for Experimental and Clinical Medicine of Federal Research Center for Basic and Translational MedicineResearch Institute for Experimental and Clinical Medicine of Federal Research Center for Basic and Translational MedicineResearch Institute for Experimental and Clinical Medicine of Federal Research Center for Basic and Translational Medicine, Novosibirsk State Medical University of Minzdrav of RussiaThe study analyzes the expression of protein genes involved in intracellular signaling pathways associated with a profibrotic response, activation of epithelial-mesenchymal and endothelial-mesenchymal transitions, in modeling tuberculous granulomatosis and pharmaceutical effects. Material and methods. The study was performed on male BALB/c mice aged two months and weighing 18–22 g. Generalized tuberculous granulomatosis was simulated by a single intravenous (retro-orbital) injection of 0.5 mg BCG vaccine in 0.2 ml of isotonic aqueous NaCl solution (SS), after 4 months a part of mice started to receive treatment drugs, after 2 months the animals were sacrificed by decapitation (6 months after BCG vaccine administration) and their lung tissues were collected. Mice were divided into 6 groups, 5 males in each: intact, which were intravenously into the retro-orbital sinus injected with 0.2 ml SS (C); infected with BCG and receiving SS intraperitoneal injections (BS); infected with BCG and received intraperitoneal injections of isonicotinic acid hydrazide solution (INAH) (BI); infected with BCG and received intraperitoneal injections of dextrazide solution (conjugate of 40 kDa oxidized dextran and INAH) (BD); infected with BCG and received intraperitoneal or inhalation injections of solution of molecular-nanosomal pharmaceutical compositions of oxidized dextran (MNPC) (BMP and BMH, respectively). Finally, the expression of mRNA of matrix metalloproteinase 9, type III procollagen, TGF-β, and transcription factors ZEB1 and Snai1 was determined by real-time PCR in mice lung tissue. Results. The modeling of generalized tuberculous granulomatosis was found to be accompanied by the induction of epithelial-mesenchymal transition and the activation of profibrotic processes, which after 6 months was manifested in increase of the type III collagen α1-chain and TGF-β mRNA expression. Administration of traditional (INAH) and original (dextrazide, MNPC) preparations with anti-tuberculosis activity within two months has inhibitory activity of varying severity in relation to various markers of these processes.https://sibmed.elpub.ru/jour/article/view/204tuberculous granulomatosisfibrosisoxidized dextrandextrazidematrix metalloproteinase 9type iii procollagentgf-βzeb1snai1 |
| spellingShingle | P. M. Kozhin A. V. Chechushkov N. S. Zaytseva M. V. Khrapova L. A. Cherdantseva E. B. Menshchikova A. V. Troitsky V. A. Shkurupy EXPRESSION OF PROTEIN GENES PARTICIPATING IN FIBROPLASTIC PROCESSES IN MICE LUNG DURING THE DEVELOPMENT OF TUBERCULOUS INFLAMMATION Сибирский научный медицинский журнал tuberculous granulomatosis fibrosis oxidized dextran dextrazide matrix metalloproteinase 9 type iii procollagen tgf-β zeb1 snai1 |
| title | EXPRESSION OF PROTEIN GENES PARTICIPATING IN FIBROPLASTIC PROCESSES IN MICE LUNG DURING THE DEVELOPMENT OF TUBERCULOUS INFLAMMATION |
| title_full | EXPRESSION OF PROTEIN GENES PARTICIPATING IN FIBROPLASTIC PROCESSES IN MICE LUNG DURING THE DEVELOPMENT OF TUBERCULOUS INFLAMMATION |
| title_fullStr | EXPRESSION OF PROTEIN GENES PARTICIPATING IN FIBROPLASTIC PROCESSES IN MICE LUNG DURING THE DEVELOPMENT OF TUBERCULOUS INFLAMMATION |
| title_full_unstemmed | EXPRESSION OF PROTEIN GENES PARTICIPATING IN FIBROPLASTIC PROCESSES IN MICE LUNG DURING THE DEVELOPMENT OF TUBERCULOUS INFLAMMATION |
| title_short | EXPRESSION OF PROTEIN GENES PARTICIPATING IN FIBROPLASTIC PROCESSES IN MICE LUNG DURING THE DEVELOPMENT OF TUBERCULOUS INFLAMMATION |
| title_sort | expression of protein genes participating in fibroplastic processes in mice lung during the development of tuberculous inflammation |
| topic | tuberculous granulomatosis fibrosis oxidized dextran dextrazide matrix metalloproteinase 9 type iii procollagen tgf-β zeb1 snai1 |
| url | https://sibmed.elpub.ru/jour/article/view/204 |
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