Quantification of Selenomonas sputigena in Chronic Periodontitis in Smokers Using 16S rDNA Based PCR Analysis
Background and Aim: Selenomonas species have been associated with chronic periodontitis and have been implicated in converting periodontal health to disease. Scanty literature is available in Indian population. Hence, the objective of the study was to detect the prevalence of Selenomonas sputige...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-04-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5782/12550_CE[Ra_AG]_F(P)_PF1(AGAK)_PFA(P)_PF2(PAG)_(P).pdf |
| Summary: | Background and Aim: Selenomonas species have been
associated with chronic periodontitis and have been implicated
in converting periodontal health to disease. Scanty literature
is available in Indian population. Hence, the objective of the
study was to detect the prevalence of Selenomonas sputigena in
healthy and chronic periodontitis by polymerase chain reaction
(PCR) in Indian population and to check whether smoking
affects the subgingival microflora of this organism in chronic
periodontitis.
Materials and Methods: A total of 60 subjects with severe
chronic periodontitis with or without smoking and periodontal
healthy subjects underwent clinical and microbiological
assessment. A deep subgingival plaque sample was collected
and genomic DNA was extracted from each sample and
analysed for detection of Selnomonas sputigena using PCR. The
frequency and quantification of bacteria were also estimated.
Results: All groups differed statistically significant in the
frequency of detection of Selenomonas sputigena. On
comparison of patients with chronic periodontitis in smokers
and non-smokers, there was no statistically significant
difference. When the results were quantified, statistically nonsignificant results were seen among all groups. Plaque index,
gingival index, probing pocket depth and clinical attachment
level were statistically non-significant in chronic periodontitis
with smokers and non-smokers.
Conclusion: Prevalence of Selenomonas sputigena showed
significant differences with respect to the frequency of detection
when comparing the disease group to the healthy population.
But no significant difference was seen when the results were
quantified. Smoking has no influence on number of Selenomonas
sputigena. This study highlights presence as well as quantity of
the organism is very important in elucidating its role in causation
and progression of the disease. |
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| ISSN: | 2249-782X 0973-709X |