Growth Performance, Immune Response, Antioxidative Status, and Antiparasitic and Antibacterial Capacity of the Nile Tilapia (Oreochromis niloticus) After Dietary Supplementation With Bottle Gourd (Lagenaria siceraria, Molina) Seed Powder
Medicinal plants are a potential safe source of bioactive compounds. Fish diet supplemented with the medicinal plant bottle gourd (Lagenaria siceraria) seed powder was evaluated in this study for the potential effect on growth performance, antioxidative status, immunological response, and resistance...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.901439/full |
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| author | Mahmoud Radwan Mahmoud Mahrous M. Abbas Amaal Mohammadein Jamila S. Al Malki Said M. A. Elraey Mohammad Magdy |
| author_facet | Mahmoud Radwan Mahmoud Mahrous M. Abbas Amaal Mohammadein Jamila S. Al Malki Said M. A. Elraey Mohammad Magdy |
| author_sort | Mahmoud Radwan |
| collection | DOAJ |
| description | Medicinal plants are a potential safe source of bioactive compounds. Fish diet supplemented with the medicinal plant bottle gourd (Lagenaria siceraria) seed powder was evaluated in this study for the potential effect on growth performance, antioxidative status, immunological response, and resistance to infectious pathogens in the Nile tilapia (Oreochromis niloticus). Nile tilapia fingerlings with mean weight ( ± SD)= (25.64 ± 0.17 g), were fed four L. siceraria seed supplemented diets (LSSD) at 0.0, 1, 2, and 3% for 60 days. Specimens were then challenged with the bacterium Aeromonas hydrophila for 10 days. Also, three different concentrations (2.5, 5, and 10 ml/L) of Lagenaria siceraria ethanolic extract (LSEE) were tested for their antibacterial and antiparasitic efficacy on four selected bacterial and one parasitic species. All parameters’ values generally improved with elevating the content of the L. siceraria seed powder in the diet. Dietary administration of LSSD-3% enabled significant (P < 0.05) higher growth performance, and feed utilization efficiency. It reduced the mortality induced by A. hydrophila infection, increased crude protein content in the fish body and exhibited the highest in vitro antibacterial and antiparasitic efficiency. RBCs, WBCs, Hb, PCV, MCV, MCH, and total serum protein values in pre- and post-challenge groups were significantly higher (P < 0.05) in the LSSD-3% group. While MCHC, ALT, AST and glucose levels were significantly lower (P < 0.05) than those of the other groups. Lysozyme and antioxidant enzyme activities in pre- and post-challenge groups were also higher (P < 0.05) in the LSSD-3% group compared to the other groups. LSEE provided good efficacy against Gram-negative bacterial strains, mild efficacy against Gram-positive bacterial strains, and an antagonistic effect on the parasite Cichlidogyrus Tilapiae. The 10 ml/L concentration was the most effective against the pathogens followed by the 5 ml/L concentration and then 2.5 ml/L. Our findings suggest the feasibility of supplementation of Nile tilapia (O. niloticus) diet with L. siceraria seed powder by 3% to improve the growth performance, immunity, and vital parameters. |
| first_indexed | 2024-12-11T18:14:32Z |
| format | Article |
| id | doaj.art-8434119a6ca2425c8c57410c8a9ebe1c |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-11T18:14:32Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Marine Science |
| spelling | doaj.art-8434119a6ca2425c8c57410c8a9ebe1c2022-12-22T00:55:28ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-06-01910.3389/fmars.2022.901439901439Growth Performance, Immune Response, Antioxidative Status, and Antiparasitic and Antibacterial Capacity of the Nile Tilapia (Oreochromis niloticus) After Dietary Supplementation With Bottle Gourd (Lagenaria siceraria, Molina) Seed PowderMahmoud Radwan0Mahmoud Mahrous M. Abbas1Amaal Mohammadein2Jamila S. Al Malki3Said M. A. Elraey4Mohammad Magdy5Marine Biology Branch, Zoology Department, Faculty of Science, Al-Azhar University, Cairo, EgyptMarine Biology Branch, Zoology Department, Faculty of Science, Al-Azhar University, Cairo, EgyptDepartment of Biology, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Biology, College of Science, Taif University, Taif, Saudi ArabiaZoology Department, Faculty of Science, Zagazig University, Zagazig, EgyptPhD Program in Evolutionary Biology and Ecology, Department of Biology, University of Rome “Tor Vergata”, Rome, ItalyMedicinal plants are a potential safe source of bioactive compounds. Fish diet supplemented with the medicinal plant bottle gourd (Lagenaria siceraria) seed powder was evaluated in this study for the potential effect on growth performance, antioxidative status, immunological response, and resistance to infectious pathogens in the Nile tilapia (Oreochromis niloticus). Nile tilapia fingerlings with mean weight ( ± SD)= (25.64 ± 0.17 g), were fed four L. siceraria seed supplemented diets (LSSD) at 0.0, 1, 2, and 3% for 60 days. Specimens were then challenged with the bacterium Aeromonas hydrophila for 10 days. Also, three different concentrations (2.5, 5, and 10 ml/L) of Lagenaria siceraria ethanolic extract (LSEE) were tested for their antibacterial and antiparasitic efficacy on four selected bacterial and one parasitic species. All parameters’ values generally improved with elevating the content of the L. siceraria seed powder in the diet. Dietary administration of LSSD-3% enabled significant (P < 0.05) higher growth performance, and feed utilization efficiency. It reduced the mortality induced by A. hydrophila infection, increased crude protein content in the fish body and exhibited the highest in vitro antibacterial and antiparasitic efficiency. RBCs, WBCs, Hb, PCV, MCV, MCH, and total serum protein values in pre- and post-challenge groups were significantly higher (P < 0.05) in the LSSD-3% group. While MCHC, ALT, AST and glucose levels were significantly lower (P < 0.05) than those of the other groups. Lysozyme and antioxidant enzyme activities in pre- and post-challenge groups were also higher (P < 0.05) in the LSSD-3% group compared to the other groups. LSEE provided good efficacy against Gram-negative bacterial strains, mild efficacy against Gram-positive bacterial strains, and an antagonistic effect on the parasite Cichlidogyrus Tilapiae. The 10 ml/L concentration was the most effective against the pathogens followed by the 5 ml/L concentration and then 2.5 ml/L. Our findings suggest the feasibility of supplementation of Nile tilapia (O. niloticus) diet with L. siceraria seed powder by 3% to improve the growth performance, immunity, and vital parameters.https://www.frontiersin.org/articles/10.3389/fmars.2022.901439/fulldietary supplementscucurbitshematological and biochemical parametersgrowth promotersimmunomodulatory dietNile tilapia feed |
| spellingShingle | Mahmoud Radwan Mahmoud Mahrous M. Abbas Amaal Mohammadein Jamila S. Al Malki Said M. A. Elraey Mohammad Magdy Growth Performance, Immune Response, Antioxidative Status, and Antiparasitic and Antibacterial Capacity of the Nile Tilapia (Oreochromis niloticus) After Dietary Supplementation With Bottle Gourd (Lagenaria siceraria, Molina) Seed Powder Frontiers in Marine Science dietary supplements cucurbits hematological and biochemical parameters growth promoters immunomodulatory diet Nile tilapia feed |
| title | Growth Performance, Immune Response, Antioxidative Status, and Antiparasitic and Antibacterial Capacity of the Nile Tilapia (Oreochromis niloticus) After Dietary Supplementation With Bottle Gourd (Lagenaria siceraria, Molina) Seed Powder |
| title_full | Growth Performance, Immune Response, Antioxidative Status, and Antiparasitic and Antibacterial Capacity of the Nile Tilapia (Oreochromis niloticus) After Dietary Supplementation With Bottle Gourd (Lagenaria siceraria, Molina) Seed Powder |
| title_fullStr | Growth Performance, Immune Response, Antioxidative Status, and Antiparasitic and Antibacterial Capacity of the Nile Tilapia (Oreochromis niloticus) After Dietary Supplementation With Bottle Gourd (Lagenaria siceraria, Molina) Seed Powder |
| title_full_unstemmed | Growth Performance, Immune Response, Antioxidative Status, and Antiparasitic and Antibacterial Capacity of the Nile Tilapia (Oreochromis niloticus) After Dietary Supplementation With Bottle Gourd (Lagenaria siceraria, Molina) Seed Powder |
| title_short | Growth Performance, Immune Response, Antioxidative Status, and Antiparasitic and Antibacterial Capacity of the Nile Tilapia (Oreochromis niloticus) After Dietary Supplementation With Bottle Gourd (Lagenaria siceraria, Molina) Seed Powder |
| title_sort | growth performance immune response antioxidative status and antiparasitic and antibacterial capacity of the nile tilapia oreochromis niloticus after dietary supplementation with bottle gourd lagenaria siceraria molina seed powder |
| topic | dietary supplements cucurbits hematological and biochemical parameters growth promoters immunomodulatory diet Nile tilapia feed |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.901439/full |
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