Cortisol and Dexamethasone Mediate Glucocorticoid Actions in the Lesser Spotted Catshark (<i>Scyliorhinus canicula</i>)
Corticosteroids are hormones produced in vertebrates exerting gluco- and mineralocorticoid actions (GC and MC) mediated by specific receptors (GR and MR, respectively). In elasmobranchs, the major circulating corticosteroid is the 1α-hydroxycorticosterone (1α-OHB). This hormone acts as a MC, but to...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/1/56 |
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| author | Juncal Cabrera-Busto Juan M. Mancera Ignacio Ruiz-Jarabo |
| author_facet | Juncal Cabrera-Busto Juan M. Mancera Ignacio Ruiz-Jarabo |
| author_sort | Juncal Cabrera-Busto |
| collection | DOAJ |
| description | Corticosteroids are hormones produced in vertebrates exerting gluco- and mineralocorticoid actions (GC and MC) mediated by specific receptors (GR and MR, respectively). In elasmobranchs, the major circulating corticosteroid is the 1α-hydroxycorticosterone (1α-OHB). This hormone acts as a MC, but to date its role as a GC has not been established. As there is no 1α-OHB standard available, here we employed a set of in vivo and ex vivo approaches to test GC actions of other corticosteroids in the lesser spotted catshark (<i>Scyliorhinus canicula</i>). Dexamethasone (DEX, a synthetic corticosteroid) slow-release implants decreased plasma 1α-OHB levels after 7 days, and modified carbohydrates metabolism in liver and white muscle (energy stores and metabolic enzymes). In addition, ex vivo culture of liver and white muscle explants confirmed GC actions of corticosteroids not naturally present in sharks (cortisol and DEX) by increasing glucose secretion from these tissues. Dose–response curves induced by cortisol and DEX, altogether with the use of specific GR inhibitor mifepristone, confirmed the involvement of GR mediating glucose secretion. This study highlights the influence of corticosteroids in the glucose balance of <i>S. canicula</i>, though the role of 1α-OHB as a GC hormone in sharks should be further confirmed. |
| first_indexed | 2024-03-10T01:54:04Z |
| format | Article |
| id | doaj.art-843665da49a14765808c99774e95b3f7 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T01:54:04Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-843665da49a14765808c99774e95b3f72023-11-23T13:00:25ZengMDPI AGBiology2079-77372021-12-011115610.3390/biology11010056Cortisol and Dexamethasone Mediate Glucocorticoid Actions in the Lesser Spotted Catshark (<i>Scyliorhinus canicula</i>)Juncal Cabrera-Busto0Juan M. Mancera1Ignacio Ruiz-Jarabo2Departament of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, 11510 Puerto Real, SpainDepartament of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, 11510 Puerto Real, SpainDepartament of Biology, Faculty of Marine and Environmental Sciences, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Cádiz, 11510 Puerto Real, SpainCorticosteroids are hormones produced in vertebrates exerting gluco- and mineralocorticoid actions (GC and MC) mediated by specific receptors (GR and MR, respectively). In elasmobranchs, the major circulating corticosteroid is the 1α-hydroxycorticosterone (1α-OHB). This hormone acts as a MC, but to date its role as a GC has not been established. As there is no 1α-OHB standard available, here we employed a set of in vivo and ex vivo approaches to test GC actions of other corticosteroids in the lesser spotted catshark (<i>Scyliorhinus canicula</i>). Dexamethasone (DEX, a synthetic corticosteroid) slow-release implants decreased plasma 1α-OHB levels after 7 days, and modified carbohydrates metabolism in liver and white muscle (energy stores and metabolic enzymes). In addition, ex vivo culture of liver and white muscle explants confirmed GC actions of corticosteroids not naturally present in sharks (cortisol and DEX) by increasing glucose secretion from these tissues. Dose–response curves induced by cortisol and DEX, altogether with the use of specific GR inhibitor mifepristone, confirmed the involvement of GR mediating glucose secretion. This study highlights the influence of corticosteroids in the glucose balance of <i>S. canicula</i>, though the role of 1α-OHB as a GC hormone in sharks should be further confirmed.https://www.mdpi.com/2079-7737/11/1/56cortisoldexamethasoneglucocorticoid receptors<i>Scyliorhinus canicula</i>sharks |
| spellingShingle | Juncal Cabrera-Busto Juan M. Mancera Ignacio Ruiz-Jarabo Cortisol and Dexamethasone Mediate Glucocorticoid Actions in the Lesser Spotted Catshark (<i>Scyliorhinus canicula</i>) Biology cortisol dexamethasone glucocorticoid receptors <i>Scyliorhinus canicula</i> sharks |
| title | Cortisol and Dexamethasone Mediate Glucocorticoid Actions in the Lesser Spotted Catshark (<i>Scyliorhinus canicula</i>) |
| title_full | Cortisol and Dexamethasone Mediate Glucocorticoid Actions in the Lesser Spotted Catshark (<i>Scyliorhinus canicula</i>) |
| title_fullStr | Cortisol and Dexamethasone Mediate Glucocorticoid Actions in the Lesser Spotted Catshark (<i>Scyliorhinus canicula</i>) |
| title_full_unstemmed | Cortisol and Dexamethasone Mediate Glucocorticoid Actions in the Lesser Spotted Catshark (<i>Scyliorhinus canicula</i>) |
| title_short | Cortisol and Dexamethasone Mediate Glucocorticoid Actions in the Lesser Spotted Catshark (<i>Scyliorhinus canicula</i>) |
| title_sort | cortisol and dexamethasone mediate glucocorticoid actions in the lesser spotted catshark i scyliorhinus canicula i |
| topic | cortisol dexamethasone glucocorticoid receptors <i>Scyliorhinus canicula</i> sharks |
| url | https://www.mdpi.com/2079-7737/11/1/56 |
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