Metagenomic next‐generation sequencing for the diagnosis of Chlamydia psittaci pneumonia
Abstract The aim of this study was to investigate the clinical characteristics of Chlamydia psittaci pneumonia and evaluate the diagnostic value of Metagenomic Next‐Generation Sequencing (mNGS). A total of 44 patients diagnosed with Chlamydia psittaci pneumonia using mNGS were retrospectively analys...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | The Clinical Respiratory Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/crj.13519 |
| _version_ | 1818162527271387136 |
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| author | Hu Li Binwei Hao Yongxue Wang Dinghong Yu Zhonghua Chen Duanlin Du Jian Xiong Kang Li Hengping Zhang Xu Liu Kai Liu Fanrong Xiao Xiaosan Cheng Lingmei Huang |
| author_facet | Hu Li Binwei Hao Yongxue Wang Dinghong Yu Zhonghua Chen Duanlin Du Jian Xiong Kang Li Hengping Zhang Xu Liu Kai Liu Fanrong Xiao Xiaosan Cheng Lingmei Huang |
| author_sort | Hu Li |
| collection | DOAJ |
| description | Abstract The aim of this study was to investigate the clinical characteristics of Chlamydia psittaci pneumonia and evaluate the diagnostic value of Metagenomic Next‐Generation Sequencing (mNGS). A total of 44 patients diagnosed with Chlamydia psittaci pneumonia using mNGS were retrospectively analysed. The demographic and clinical features, laboratory data, imaging findings and clinical outcomes were collected. Results showed that 65.91% of the patients had a history of exposure to poultry or birds. All patients presented with fever. Apart from systemic and respiratory symptoms, some patients also presented with digestive and neurological symptoms. Respiratory failure was common among patients. The key laboratory tests were normal white blood cell counts, slightly elevated PCT, changes in levels of cardiac enzymes, liver enzymes and hyponatremia. Chest imaging revealed that most of the lesions contained patchy exudation or lobar consolidation of one lobe, especially in the lower lobe. Consolidation of both lungs was seen in critically ill patients. Although quinolones were effective in most patients, tetracyclines should be the first choice of treatment. The overall prognosis was good; however, patients who developed severe pneumonia had poor prognosis. The incidence of chlamydia psittaci pneumonia may be underestimated due to the nonspecific clinical manifestations and lack of confirmatory testing methods. The use of mNGS has increased the number of patients diagnosed with chlamydia psittaci pneumonia. mNGS is an effective diagnostic method for chlamydia psittaci pneumonia. |
| first_indexed | 2024-12-11T16:35:05Z |
| format | Article |
| id | doaj.art-8436d200b111414d8015a43e509b0618 |
| institution | Directory Open Access Journal |
| issn | 1752-6981 1752-699X |
| language | English |
| last_indexed | 2024-12-11T16:35:05Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Clinical Respiratory Journal |
| spelling | doaj.art-8436d200b111414d8015a43e509b06182022-12-22T00:58:29ZengWileyThe Clinical Respiratory Journal1752-69811752-699X2022-07-0116751352110.1111/crj.13519Metagenomic next‐generation sequencing for the diagnosis of Chlamydia psittaci pneumoniaHu Li0Binwei Hao1Yongxue Wang2Dinghong Yu3Zhonghua Chen4Duanlin Du5Jian Xiong6Kang Li7Hengping Zhang8Xu Liu9Kai Liu10Fanrong Xiao11Xiaosan Cheng12Lingmei Huang13Department of Imaging Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital Third Hospital of Shanxi Medical University Taiyuan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaDepartment of Pulmonary and Critical Care Medicine Yueyang Central Hospital Yueyang Hunan ChinaAbstract The aim of this study was to investigate the clinical characteristics of Chlamydia psittaci pneumonia and evaluate the diagnostic value of Metagenomic Next‐Generation Sequencing (mNGS). A total of 44 patients diagnosed with Chlamydia psittaci pneumonia using mNGS were retrospectively analysed. The demographic and clinical features, laboratory data, imaging findings and clinical outcomes were collected. Results showed that 65.91% of the patients had a history of exposure to poultry or birds. All patients presented with fever. Apart from systemic and respiratory symptoms, some patients also presented with digestive and neurological symptoms. Respiratory failure was common among patients. The key laboratory tests were normal white blood cell counts, slightly elevated PCT, changes in levels of cardiac enzymes, liver enzymes and hyponatremia. Chest imaging revealed that most of the lesions contained patchy exudation or lobar consolidation of one lobe, especially in the lower lobe. Consolidation of both lungs was seen in critically ill patients. Although quinolones were effective in most patients, tetracyclines should be the first choice of treatment. The overall prognosis was good; however, patients who developed severe pneumonia had poor prognosis. The incidence of chlamydia psittaci pneumonia may be underestimated due to the nonspecific clinical manifestations and lack of confirmatory testing methods. The use of mNGS has increased the number of patients diagnosed with chlamydia psittaci pneumonia. mNGS is an effective diagnostic method for chlamydia psittaci pneumonia.https://doi.org/10.1111/crj.13519Chlamydia psittaciclinical analysismetagenomic next‐generation sequencingpneumonia |
| spellingShingle | Hu Li Binwei Hao Yongxue Wang Dinghong Yu Zhonghua Chen Duanlin Du Jian Xiong Kang Li Hengping Zhang Xu Liu Kai Liu Fanrong Xiao Xiaosan Cheng Lingmei Huang Metagenomic next‐generation sequencing for the diagnosis of Chlamydia psittaci pneumonia The Clinical Respiratory Journal Chlamydia psittaci clinical analysis metagenomic next‐generation sequencing pneumonia |
| title | Metagenomic next‐generation sequencing for the diagnosis of Chlamydia psittaci pneumonia |
| title_full | Metagenomic next‐generation sequencing for the diagnosis of Chlamydia psittaci pneumonia |
| title_fullStr | Metagenomic next‐generation sequencing for the diagnosis of Chlamydia psittaci pneumonia |
| title_full_unstemmed | Metagenomic next‐generation sequencing for the diagnosis of Chlamydia psittaci pneumonia |
| title_short | Metagenomic next‐generation sequencing for the diagnosis of Chlamydia psittaci pneumonia |
| title_sort | metagenomic next generation sequencing for the diagnosis of chlamydia psittaci pneumonia |
| topic | Chlamydia psittaci clinical analysis metagenomic next‐generation sequencing pneumonia |
| url | https://doi.org/10.1111/crj.13519 |
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