Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic <i>Brassica napus</i> and <i>B. rapa</i> L. ssp.
Interspecific hybridization between transgenic crops and their wild relatives is a major concern for transgene dispersal in the environment. Under controlled conditions, artificial hand pollination experiments were performed in order to assess the hybridization potential and the fitness of interspec...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Soo-In Sohn Senthil Kumar Thamilarasan Subramani Pandian Young-Ju Oh Hyeon-Jung Kang Eun-Kyoung Shin |
| author_facet | Soo-In Sohn Senthil Kumar Thamilarasan Subramani Pandian Young-Ju Oh Hyeon-Jung Kang Eun-Kyoung Shin |
| author_sort | Soo-In Sohn |
| collection | DOAJ |
| description | Interspecific hybridization between transgenic crops and their wild relatives is a major concern for transgene dispersal in the environment. Under controlled conditions, artificial hand pollination experiments were performed in order to assess the hybridization potential and the fitness of interspecific hybrids between <i>Brassica rapa</i> and genetically modified (GM) <i>Brassica napus</i>. Initially, six subspecies of <i>B. rapa</i> were hybridized with GM <i>B. napus</i> through hand pollination. In the resulting F<sub>1</sub> hybrids, the combination of <i>B. rapa</i> ssp. <i>narinosa</i> (♀) × GM <i>B. napus</i> (♂) had the highest crossability index (16.9 ± 2.6). However, the F<sub>1</sub> selfing progenies of <i>B. rapa</i> ssp. <i>rapa</i> (♀) × GM <i>B. napus</i> were found to be more effective in producing viable future generations with the highest crossability index (1.6 ± 0.69) compared to other subspecies. Consequently, they were used for the generation of F<sub>2</sub> and F<sub>3</sub> progenies. The 18 different morphological characteristics among the parental cross-combinations and F<sub>1</sub> hybrid progenies were measured and visualized through hierarchical clustering. Different generations were found to be grouped based on their different morphological characteristics. The chromosome numbers among the interspecific hybrids ranged from 2n = 29 to 2n = 40. Furthermore, the SSR markers revealed the presence of genomic portions in the hybrids in comparison with their parental lines. There is a high possibility of transgene flow between GM <i>B. napus</i> and <i>B. rapa</i>. The study concluded that the interspecific hybrids between <i>B. napus</i> and <i>B. rapa</i> can be viable and can actively hybridize up to F<sub>3</sub> generations and more. This suggests that the GM <i>B. napus</i> can disperse the transgene into <i>B. rapa</i>, and that it can pass through for several generations by hand pollination in a greenhouse environment. |
| first_indexed | 2024-03-09T23:45:53Z |
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| id | doaj.art-84377807443d47719708714283820e6a |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:45:53Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-84377807443d47719708714283820e6a2023-11-23T16:43:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181051210.3390/ijms231810512Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic <i>Brassica napus</i> and <i>B. rapa</i> L. ssp.Soo-In Sohn0Senthil Kumar Thamilarasan1Subramani Pandian2Young-Ju Oh3Hyeon-Jung Kang4Eun-Kyoung Shin5Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaInstitute for Future Environment Ecology Co., Ltd., Jeonju 54883, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaInterspecific hybridization between transgenic crops and their wild relatives is a major concern for transgene dispersal in the environment. Under controlled conditions, artificial hand pollination experiments were performed in order to assess the hybridization potential and the fitness of interspecific hybrids between <i>Brassica rapa</i> and genetically modified (GM) <i>Brassica napus</i>. Initially, six subspecies of <i>B. rapa</i> were hybridized with GM <i>B. napus</i> through hand pollination. In the resulting F<sub>1</sub> hybrids, the combination of <i>B. rapa</i> ssp. <i>narinosa</i> (♀) × GM <i>B. napus</i> (♂) had the highest crossability index (16.9 ± 2.6). However, the F<sub>1</sub> selfing progenies of <i>B. rapa</i> ssp. <i>rapa</i> (♀) × GM <i>B. napus</i> were found to be more effective in producing viable future generations with the highest crossability index (1.6 ± 0.69) compared to other subspecies. Consequently, they were used for the generation of F<sub>2</sub> and F<sub>3</sub> progenies. The 18 different morphological characteristics among the parental cross-combinations and F<sub>1</sub> hybrid progenies were measured and visualized through hierarchical clustering. Different generations were found to be grouped based on their different morphological characteristics. The chromosome numbers among the interspecific hybrids ranged from 2n = 29 to 2n = 40. Furthermore, the SSR markers revealed the presence of genomic portions in the hybrids in comparison with their parental lines. There is a high possibility of transgene flow between GM <i>B. napus</i> and <i>B. rapa</i>. The study concluded that the interspecific hybrids between <i>B. napus</i> and <i>B. rapa</i> can be viable and can actively hybridize up to F<sub>3</sub> generations and more. This suggests that the GM <i>B. napus</i> can disperse the transgene into <i>B. rapa</i>, and that it can pass through for several generations by hand pollination in a greenhouse environment.https://www.mdpi.com/1422-0067/23/18/10512<i>Brassica napus</i><i>Brassica rapa</i>genetically modified cropsinterspecific hybridizationtransgene persistenceSSR markers |
| spellingShingle | Soo-In Sohn Senthil Kumar Thamilarasan Subramani Pandian Young-Ju Oh Hyeon-Jung Kang Eun-Kyoung Shin Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic <i>Brassica napus</i> and <i>B. rapa</i> L. ssp. International Journal of Molecular Sciences <i>Brassica napus</i> <i>Brassica rapa</i> genetically modified crops interspecific hybridization transgene persistence SSR markers |
| title | Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic <i>Brassica napus</i> and <i>B. rapa</i> L. ssp. |
| title_full | Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic <i>Brassica napus</i> and <i>B. rapa</i> L. ssp. |
| title_fullStr | Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic <i>Brassica napus</i> and <i>B. rapa</i> L. ssp. |
| title_full_unstemmed | Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic <i>Brassica napus</i> and <i>B. rapa</i> L. ssp. |
| title_short | Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic <i>Brassica napus</i> and <i>B. rapa</i> L. ssp. |
| title_sort | characteristics and fitness analysis through interspecific hybrid progenies of transgenic i brassica napus i and i b rapa i l ssp |
| topic | <i>Brassica napus</i> <i>Brassica rapa</i> genetically modified crops interspecific hybridization transgene persistence SSR markers |
| url | https://www.mdpi.com/1422-0067/23/18/10512 |
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