The Role of Epigenetics in the Induction of Fetal Hemoglobin: A Combination Therapy Approach
Background: β-thalassemia considers worldwide public health disorders. Novel fetal hemoglobin inducer agents such as thalidomide and sodium butyrate have been attended for ameliorating clinical complications of such disorders. Material and Methods: We used thalidomide and sodium butyrate for increas...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2014-03-01
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| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
| Subjects: | |
| Online Access: | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/433 |
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| author | Mohammad Ahmadvand Mehrdad Noruzinia Ali Dehghani Fard Mostafa Montazer Zohour Mohammad Amin Tabatabaiefar Masoud Soleimani Saeid Kaviani Saeid Abroun Sahar Beiranvand Najmaldin Saki |
| author_facet | Mohammad Ahmadvand Mehrdad Noruzinia Ali Dehghani Fard Mostafa Montazer Zohour Mohammad Amin Tabatabaiefar Masoud Soleimani Saeid Kaviani Saeid Abroun Sahar Beiranvand Najmaldin Saki |
| author_sort | Mohammad Ahmadvand |
| collection | DOAJ |
| description | Background: β-thalassemia considers worldwide public health disorders. Novel fetal hemoglobin inducer agents such as thalidomide and sodium butyrate have been attended for ameliorating clinical complications of such disorders.
Material and Methods: We used thalidomide and sodium butyrate for increasing the level of fetal hemoglobin in erythroid progenitors. Briefly, after isolation of CD133+ stem cells from umbilical cord blood and differentiation into erythroid lineage, erythroid progenitors were treated with thalidomide and sodium butyraye as single and combination. H3K4 histone methylation was evaluated following fetal hemoglobin induction using chromatin immuno percipitation (ChIP) technique.
Results: The results of this study showed that the effect of thalidomide on increasing of H3K4 methylation was highest compared to sodium butyrate and combination of both agents (p < 0.05).
Conclusion: Consequently, our study of the epigenetic modification of the γ-globin suggests that histone H3K4 dimethylation are significant for the regulation of developmental stage-specific expression of the γ-globin genes. |
| first_indexed | 2024-03-12T08:22:49Z |
| format | Article |
| id | doaj.art-843838cdfef14149a5a25d49a3d7b621 |
| institution | Directory Open Access Journal |
| issn | 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T08:22:49Z |
| publishDate | 2014-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-843838cdfef14149a5a25d49a3d7b6212023-09-02T18:18:19ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-22072014-03-0181419The Role of Epigenetics in the Induction of Fetal Hemoglobin: A Combination Therapy ApproachMohammad Ahmadvand0Mehrdad Noruzinia1Ali Dehghani Fard2Mostafa Montazer Zohour3Mohammad Amin Tabatabaiefar4Masoud Soleimani5Saeid Kaviani6Saeid Abroun7Sahar Beiranvand8Najmaldin Saki9Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Department of Medical Genetics, School of Medicine, Tarbiat Modares University, Tehran, Iran.Sarem Cell Research Center- SCRC, Sarem Women's Hospital, Tehran, Iran.Genetics of noncommunicable disease research center, Zahedan University of Medical Science, Zahedan, Iran.Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Sarem Cell Research Center- SCRC, Sarem Women's Hospital, Tehran, Iran.Health research center, Research Center of Thalassemia & Hemoglobinopathy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Background: β-thalassemia considers worldwide public health disorders. Novel fetal hemoglobin inducer agents such as thalidomide and sodium butyrate have been attended for ameliorating clinical complications of such disorders. Material and Methods: We used thalidomide and sodium butyrate for increasing the level of fetal hemoglobin in erythroid progenitors. Briefly, after isolation of CD133+ stem cells from umbilical cord blood and differentiation into erythroid lineage, erythroid progenitors were treated with thalidomide and sodium butyraye as single and combination. H3K4 histone methylation was evaluated following fetal hemoglobin induction using chromatin immuno percipitation (ChIP) technique. Results: The results of this study showed that the effect of thalidomide on increasing of H3K4 methylation was highest compared to sodium butyrate and combination of both agents (p < 0.05). Conclusion: Consequently, our study of the epigenetic modification of the γ-globin suggests that histone H3K4 dimethylation are significant for the regulation of developmental stage-specific expression of the γ-globin genes.https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/433Sodium butyrateThalidomideβ-thalassemiaγ-globin |
| spellingShingle | Mohammad Ahmadvand Mehrdad Noruzinia Ali Dehghani Fard Mostafa Montazer Zohour Mohammad Amin Tabatabaiefar Masoud Soleimani Saeid Kaviani Saeid Abroun Sahar Beiranvand Najmaldin Saki The Role of Epigenetics in the Induction of Fetal Hemoglobin: A Combination Therapy Approach International Journal of Hematology-Oncology and Stem Cell Research Sodium butyrate Thalidomide β-thalassemia γ-globin |
| title | The Role of Epigenetics in the Induction of Fetal Hemoglobin: A Combination Therapy Approach |
| title_full | The Role of Epigenetics in the Induction of Fetal Hemoglobin: A Combination Therapy Approach |
| title_fullStr | The Role of Epigenetics in the Induction of Fetal Hemoglobin: A Combination Therapy Approach |
| title_full_unstemmed | The Role of Epigenetics in the Induction of Fetal Hemoglobin: A Combination Therapy Approach |
| title_short | The Role of Epigenetics in the Induction of Fetal Hemoglobin: A Combination Therapy Approach |
| title_sort | role of epigenetics in the induction of fetal hemoglobin a combination therapy approach |
| topic | Sodium butyrate Thalidomide β-thalassemia γ-globin |
| url | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/433 |
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